A0A098D1N7 · GRA6_GIBZE

Function

function

Part of the gene cluster that mediates the biosynthesis of gramillins A and B, bicyclic lipopeptides that induce cell death in maize leaves but not in wheat leaves (PubMed:30395461).
The nonribosomal peptide synthetase GRA1 incorporates respectively a glutamic adic (Glu), a leucine (Leu), a serine (Ser), a hydroxyglutamine (HOGln), a 2-amino decanoic acid, and 2 cysteins (CysB and CysA) (Probable). The biosynthesis of 2-amino decanoic acid incorporated in gramillins could be initiated by a fatty acid synthase composed of the alpha and beta subunits FGSG_00036 and FGSG_11656 (Probable). The cytochrome P450 monooxygenase FGSG_15680 could hydroxylate the fatty acid chain (Probable). Subsequent oxidation to the ketone by the oxidoreductase FGSG_00048 and transamination by aminotransferase FGSG_00049 could form 2-amino-decanoic acid (Probable). On the other hand, FGSG_15680 could also be responsible for the HO-modified glutamine at the gamma-position (Probable). Whether hydroxylation occurs on the fully assembled product or on the Gln residue prior to assembly into the gramillins requires further proof (Probable). The thioredoxin FGSG_00043 could also be required for the disulfide-bond formation between CysA and CysB (Probable). The specific involvement of the remaining proteins from the cluster is more difficult to discern, but could have broader regulatory (FGSG_00040 and FGSG_11657) or enzymatic functions (FGSG_00044 and FGSG_00045) (Probable). The final C-domain of GRA1 does not possess the expected sequence of a termination CT domain, often implicated in macrocyclization and release of a cyclopeptidein fungal NRPs; and the thioesterase FGSG_00047 may act in concert with the terminal C-domain of GRA1 to catalyze the formation of the macrocyclic anhydride and release of the products (Probable)

Pathway

Mycotoxin biosynthesis.

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Gramillins biosynthetic cluster protein FGSG_00038

Gene names

    • ORF names
      FG00038, FGRAMPH1_01T00133, FGSG_00038

Organism names

Accessions

  • Primary accession
    A0A098D1N7
  • Secondary accessions
    • A0A0E0RKU1

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004505711-203Gramillins biosynthetic cluster protein FGSG_00038

Family & Domains

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    203
  • Mass (Da)
    22,296
  • Last updated
    2015-01-07 v1
  • Checksum
    E78BFC75700BA184
MSNIAHGNPQKRPGYAIDIEASCRKRGKSAPAEDCLNEAKTPLDEIESRVVALQRQIANLNSGTLLQSIKEATARLATSWALVTKHQRYVDGYQELALPDAPTYHVQALKTAQSDLEKASQDAQAADGVLASAQKEQRDFKRVEENLVMLGAERATLDQSVRDLTLDKERCDVYLGMVEYGPDGLATLLEKDVGAWKGMLDLV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HG970332
EMBL· GenBank· DDBJ
CEF71866.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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