A0A097J6N2 · A0A097J6N2_BPR32

Function

function

The terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome to initiate and to end a packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer once the capsid is full (headful packaging). Once the capsid is packaged with the DNA, the terminase complex is substituted by the tail.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: ATPase activity requires 1 Mg2+ ion per subunit. Nuclease activity probably requires 2 Mg2+ ions per subunit.

Activity regulation

Stimulated up to 50 to 100-fold by the terminase small subunit.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site138ATP (UniProtKB | ChEBI)
Binding site143ATP (UniProtKB | ChEBI)
Binding site202ATP (UniProtKB | ChEBI)
Active site256For ATPase activity
Binding site401Mg2+ 2 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site401Mg2+ 1 (UniProtKB | ChEBI); catalytic; for nuclease activity
Site409Modulates nuclease activity
Binding site458Mg2+ 2 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site542Mg2+ 1 (UniProtKB | ChEBI); catalytic; for nuclease activity

GO annotations

AspectTerm
Cellular Componentviral terminase, large subunit
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functionendonuclease activity
Molecular Functionmetal ion binding
Biological Processchromosome organization
Biological Processviral DNA genome packaging

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Terminase, large subunit
  • Alternative names
    • DNA-packaging protein

Including 2 domains:

  • Recommended name
    ATPase
  • EC number
  • Recommended name
    Endonuclease
  • EC number

Gene names

    • ORF names
      RB33_166

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Tevenvirinae > Tequatrovirus > Enterobacteria phage RB32

Accessions

  • Primary accession
    A0A097J6N2

Proteomes

Subcellular Location

Interaction

Subunit

Interacts with the terminase small subunit; the active complex is composed of a pentamer of terminase large subunits and a dodecamer of terminase small subunits. Interacts with the portal protein.

Family & Domains

Features

Showing features for region, motif, domain.

TypeIDPosition(s)Description
Region131-301ATPase activity
Motif161-167Walker A motif
Motif251-256Walker B motif
Motif285-287ATPase coupling
Region360-559Nuclease activity
Domain399-572Terminase large subunit gp17-like C-terminal

Domain

The N-terminus contains an ATPase domain. The C-terminus contains an endonuclease domain.

Sequence similarities

Belongs to the Tequatrovirus large terminase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    610
  • Mass (Da)
    69,784
  • Last updated
    2015-01-07 v1
  • Checksum
    7B372AC2B6F27FA9
MEQPINALNDFHPLNEAGKILIKHPSLAERKDEDGIHWIKSQWDGKWYPEKFSDYLRLHKIVKIPNNSDKPELFQTYKDKNNKRSRYMGLPNLKRANIKTQWTREMVEEWKKCRDDIVYFAETYCAITHIDYGVIKVQLRDYQRDMLKIMSSKRMTVCNLSRQLGKTTVVAIFLAHFVCFNKDKAVGILAHKGSMSAEVLDRTKQAIELLPDFLQPGIVEWNKGSIELDNGSSIGAYASSPDAVRGNSFAMIYIDECAFIPNFHDSWLAIQPVISSGRRSKIIITTTPNGLNHFYDIWTAAVEGKSGFEPYTAIWNSVKERLYNDEDIFDDGWQWSIQTINGSTLAQFRQEHTAAFEGTSGTLISGMKLAIMDFIEVTPDDHGFHRFKKPEPDRKYIATLDCSEGRGQDYHALHIIDVTDDVWEQVGVLHSNTISHLILPDIVMRYLVEYNECPVYIELNSTGVSVAKSLYMDLEYEGVICDSYTDLGMKQTKRTKAVGCSTLKDLIEKDKLIIHHRATIQEFRTFSEKGVSWAAEEGYHDDLVMSLVIFGWLSTQSKFIDYADKDDMRLASEVFSKELQDMSDDYAPVIFVDSVHSAEYVPVSHGMSMV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KM607001
EMBL· GenBank· DDBJ
AIT74804.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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