A0A095AEX1 · A0A095AEX1_SCHHA
- ProteinUDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids423 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transfering GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.
Catalytic activity
- a di-trans,poly-cis-dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UMPThis reaction proceeds in the forward direction.
a di-trans,poly-cis-dolichyl phosphate RHEA-COMP:19498 + CHEBI:57705 = an N-acetyl-α-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol RHEA-COMP:19507 + CHEBI:57865
Cofactor
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | glycosyltransferase activity | |
Molecular Function | UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity | |
Biological Process | dolichol-linked oligosaccharide biosynthetic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Spiralia > Lophotrochozoa > Platyhelminthes > Trematoda > Digenea > Strigeidida > Schistosomatoidea > Schistosomatidae > Schistosoma
Accessions
- Primary accessionA0A095AEX1
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 20-38 | Helical | ||||
Sequence: FFELLVLVCMSIFGAKYVL | ||||||
Transmembrane | 72-93 | Helical | ||||
Sequence: VICGVVFLSIMFLFIPVPFWRY | ||||||
Transmembrane | 105-124 | Helical | ||||
Sequence: FIQFLAGLLSICCMLFLGFA | ||||||
Transmembrane | 136-165 | Helical | ||||
Sequence: IGFPFVAGLPLLMVYLANEGTTSIAVPVML | ||||||
Transmembrane | 177-197 | Helical | ||||
Sequence: ILYYIYMGLLTVFCTNSINIY | ||||||
Transmembrane | 209-235 | Helical | ||||
Sequence: IVIGASLILFNLIELSSMTFAVVGILG | ||||||
Transmembrane | 241-263 | Helical | ||||
Sequence: LLLFFLPQIVNFLYSTPQLFGLI | ||||||
Transmembrane | 395-418 | Helical | ||||
Sequence: LLLLQILCSCFAFMIRYPLAWLFY |
Keywords
- Cellular component
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 329-361 | Disordered | ||||
Sequence: MIVSQSSHENMSSDEEQTSERQRARSRSPAQTK | ||||||
Compositional bias | 341-355 | Basic and acidic residues | ||||
Sequence: SDEEQTSERQRARSR |
Sequence similarities
Belongs to the glycosyltransferase 4 family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length423
- Mass (Da)47,134
- Last updated2014-11-26 v1
- Checksum3AC3D0D8716CED69
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 341-355 | Basic and acidic residues | ||||
Sequence: SDEEQTSERQRARSR |