A0A093V5I5 · A0A093V5I5_TALMA
- ProteinDecapping nuclease
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids367 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA. The NAD-cap is present at the 5'-end of some RNAs and snoRNAs. In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs. Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1). Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates. Stimulates exoribonuclease activity of Rat1, allowing it to degrade RNAs with stable secondary structure more effectively.
Catalytic activity
- a 5'-end (N7-methyl 5'-triphosphoguanosine)-ribonucleoside-ribonucleotide in mRNA + H2O = a (N7-methyl 5'-triphosphoguanosine)-nucleoside + a 5'-end phospho-ribonucleoside in mRNA + H+This reaction proceeds in the forward direction.
- a 5'-end NAD+-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + NAD+ + H+This reaction proceeds in the forward direction.
- a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + diphosphate + H+This reaction proceeds in the forward direction.
Cofactor
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Molecular Function | metal ion binding | |
Molecular Function | mRNA 5'-diphosphatase activity | |
Molecular Function | nuclease activity | |
Molecular Function | nucleotide binding | |
Molecular Function | RNA binding | |
Biological Process | NAD-cap decapping | |
Biological Process | nuclear-transcribed mRNA catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDecapping nuclease
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Trichocomaceae > Talaromyces > Talaromyces sect. Talaromyces
Accessions
- Primary accessionA0A093V5I5
Proteomes
Subcellular Location
Structure
Sequence
- Sequence statusComplete
- Length367
- Mass (Da)42,376
- Last updated2014-11-26 v1
- ChecksumC239BCC6CAD10C2F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JPOX01000051 EMBL· GenBank· DDBJ | KFX41971.1 EMBL· GenBank· DDBJ | Genomic DNA |