A0A090WIX7 · A0A090WIX7_9FLAO
- ProteinMultifunctional fusion protein
- GenegatB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1111 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).
Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).
Catalytic activity
- ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H+ + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate
- ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H+ + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate
Features
Showing features for site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 548 | Important for tRNA non-discrimination | ||||
Sequence: H | ||||||
Binding site | 692 | L-aspartate (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 738 | L-aspartate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 738-740 | ATP (UniProtKB | ChEBI) | ||||
Sequence: RDE | ||||||
Binding site | 747 | ATP (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 969 | L-aspartate (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 1007 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 1014 | L-aspartate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 1059-1062 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GVDR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | aspartate-tRNA ligase activity | |
Molecular Function | aspartate-tRNA(Asn) ligase activity | |
Molecular Function | ATP binding | |
Molecular Function | glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity | |
Molecular Function | nucleic acid binding | |
Molecular Function | transferase activity | |
Biological Process | aspartyl-tRNA aminoacylation | |
Biological Process | glutaminyl-tRNAGln biosynthesis via transamidation |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameMultifunctional fusion protein
Including 2 domains:
- Recommended nameAspartate--tRNA(Asp/Asn) ligase
- EC number
- Alternative names
- Recommended nameAspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
- EC number
- Short namesAsp/Glu-ADT subunit B
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacteroidota > Flavobacteriia > Flavobacteriales > Flavobacteriaceae > Jejuia
Accessions
- Primary accessionA0A090WIX7
Proteomes
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 662-1080 | Aminoacyl-transfer RNA synthetases class-II family profile | ||||
Sequence: RHKVIKLMRDLLDEQEFLEVETPILTAGTDEGAREFIVPTRKKAGSFYTLPQAPQQFKQILMVSGYEKYFQIARCFRDEDSRGDRQPEFTQLDIEMAYASMQDIIDLSTNMFNEIVTRIYGKKWILHPFEVLTYKEAMDYYGCDRPDLRFGLKLQDITNIVKDTTFQVFSKPIDEGGIVKCIRVSVEEQGKKRLSKGQIEKLTGIAQEHGLGGLAYIIVNENDLQSPIIKFLGEDIAKNIIKTTNAQVGDIVFFSAADYETANKALDAVRQELGSMLKLINPKELRPAWVVDFPMFEKTEEGNWTFTHNPFSMPAVKDIEKHINGKEEEIGSIIAQQYDLILNGFEIGGGSVRAHKPEILEATYKNMGYNKEDMMKSVGTMYNAFHYGAPPHGGIAWGVDRLMMILEKKASIREVMAFP | ||||||
Region | 716-719 | Aspartate | ||||
Sequence: QQFK |
Sequence similarities
Belongs to the GatB/GatE family. GatB subfamily.
Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,111
- Mass (Da)125,888
- Last updated2014-11-26 v1
- ChecksumF42C9CFF7FA2C61F