A0A090WAE7 · A0A090WAE7_9FLAO
- ProteintRNA modification GTPase MnmE
- GenemnmE
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids377 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.
Cofactor
Note: Binds 1 potassium ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 23 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 86 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 125 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 231 | K+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 231-236 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NVGKST | ||||||
Binding site | 235 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 250 | K+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 250-256 | GTP (UniProtKB | ChEBI) | ||||
Sequence: SEIAGTT | ||||||
Binding site | 252 | K+ (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 255 | K+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 256 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 275-278 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DTAG | ||||||
Binding site | 376 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | metal ion binding | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA modification GTPase MnmE
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacteroidota > Flavobacteriia > Flavobacteriales > Flavobacteriaceae > Jejuia
Accessions
- Primary accessionA0A090WAE7
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 221-377 | TrmE-type G | ||||
Sequence: GIPVAIVGEPNVGKSTLLNALLNEERAIVSEIAGTTRDTIEDEITIGGIGFRFIDTAGIRETKDVVESIGIKKTFEKIEQAQVVVYLFEADVISRDTEKSKAIRLEVEKIKNKYPQKPLIVLANKVDKISEHEILDLNTQIPELQFISAKLRLVLKN |
Sequence similarities
Family and domain databases
Sequence
- Sequence statusComplete
- Length377
- Mass (Da)41,522
- Last updated2014-11-26 v1
- Checksum14C367555D682DE9