A0A090CSU6 · A0A090CSU6_PODAN

Function

function

NADH-dependent reductase for DPH3 and cytochrome b5. Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1. By reducing DPH3, also involved in the formation of the tRNA wobble base modification mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), mediated by the elongator complex. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Pathway

Protein modification; peptidyl-diphthamide biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site117FAD (UniProtKB | ChEBI)
Binding site119FAD (UniProtKB | ChEBI)
Binding site134FAD (UniProtKB | ChEBI)
Binding site136FAD (UniProtKB | ChEBI)
Binding site143FAD (UniProtKB | ChEBI)
Binding site144FAD (UniProtKB | ChEBI)
Binding site185FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Cellular Componentmitochondrial outer membrane
Molecular Functioncytochrome-b5 reductase activity, acting on NAD(P)H
Molecular Functiontransferase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADH-cytochrome b5 reductase
  • EC number

Organism names

Accessions

  • Primary accession
    A0A090CSU6

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane32-49Helical

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain60-168FAD-binding FR-type

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    309
  • Mass (Da)
    33,747
  • Last updated
    2014-11-26 v1
  • Checksum
    D054DC16F946A315
MSDSLLAKKYVDGIYIPAGLIVLGTAILKREFLHYAVLVAIALGAIKFIRTQPKKVLKPDAFQEFELKEKTIISHNVAIYRFALPNPSDILGLPIGQHISIGAHLPQPDGTTKEIVRSYTPVSGDHQPGYFDLLIKSYPTGNISKHMAGLAVGQTIRVKGPKGAFVYTPNMVRHFGMIAGGTGITPMLQVIRAIVRGRKAGDTTQVDLIFANVTKEDILLKEDLDALTKEDKGIRVHYVLDKPPADWQGGVGYVTGDMITKWLPKPAEDVKLLLCGPPPMVSGLKKTAEALGFKKARPVSKLEDQIFAF

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FO904942
EMBL· GenBank· DDBJ
CDP31905.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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