A0A089FUV9 · A0A089FUV9_9GEMI

Function

function

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the + strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Divalent metal cations, possibly Mg2+ or Mn2+.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site49a divalent metal cation (UniProtKB | ChEBI)
Binding site57a divalent metal cation (UniProtKB | ChEBI)
Binding site59a divalent metal cation (UniProtKB | ChEBI)
Active site103For DNA cleavage activity

GO annotations

AspectTerm
Cellular Componenthost cell nucleus
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular Functionendodeoxyribonuclease activity, producing 5'-phosphomonoesters
Molecular Functionhelicase activity
Molecular Functionmetal ion binding
Molecular Functionnucleotidyltransferase activity
Molecular Functionstructural molecule activity
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Replication-associated protein
  • EC number
  • Short names
    Rep

Gene names

    • Name
      AC1

Organism names

Accessions

  • Primary accession
    A0A089FUV9

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Subunit

Homooligomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain8-116CRESS-DNA virus Rep endonuclease

Domain

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.

Sequence similarities

Belongs to the geminiviridae Rep protein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    352
  • Mass (Da)
    39,915
  • Last updated
    2014-11-26 v1
  • Checksum
    662BEFE24BDD1177
MPRAGRFQINAKNYFITYPRCSLTKEEALSQLKALSYPTNIKFVRVCRELHQDGVPHLHVLIQFEGKFQCTNPRFFDLISPSRSTHFHPNIQGAKSSSDVKAYIEKGGEFLDDGIFQVDARSARGEGQHLAQVYADALNASSKSEALNIIKEKDPKSFFLQFHNISANADRIFQAPPQTYVSPFLSSSFTQIPEEIEVWVSENICRPAARPWRPISIVLEGDSRTGKTMWARSLGPHNYLCGHLDLSPKIYSNDAWYNVIDDVDPHYLKHFKEFMGAQRDWQSNTKYGKPIQIKGGIPTIFLCNPGPNSSYKEYLDEDKNSNLKNWAIKNALFISLTEPLFSSTDQSQAQAS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KJ888092
EMBL· GenBank· DDBJ
AIP89857.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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