A0A085GB02 · A0A085GB02_EWIA3

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site300Mg2+ (UniProtKB | ChEBI); catalytic
Binding site343Mg2+ (UniProtKB | ChEBI); catalytic
Binding site401Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site404Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      GEAM_2219

Organism names

Accessions

  • Primary accession
    A0A085GB02

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain36-116S1 motif
Region401-404Required for zinc-mediated homotetramerization and catalytic activity
Region534-558Disordered
Region583-759Disordered
Compositional bias589-720Basic and acidic residues
Compositional bias739-759Basic and acidic residues
Region783-842Disordered
Compositional bias805-819Basic and acidic residues
Region1073-1105Disordered

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,105
  • Mass (Da)
    122,527
  • Last updated
    2014-10-29 v1
  • Checksum
    77A3CED7BB5B80A5
MLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKGKITRIEPSLEAAFVDYGAERHGFLPLKEISREYFPSNYSSHGRPNIKDVLREGQEVIVQVDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALSSLQLPDGMGLIVRTAGVGKSAEALQWDLTFRLKHWDAIKKAAEGRPAPFLIHQESNVIVRAFRDYLRPDIGEILIDNPKILDLAKEHIAALGRPDFSSKIKPYIGEIPLFSHYQIESQIESAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQLRLRDLGGLIVIDFIDMTPVRHQREVENRLRDAVRQDRARIQIGRISRFGLLEMSRQRLSPSLGESTHHICPRCAGTGTIRDNESLSLSILRLIEEEALKENTKEVHAIVPVPIASYLLNEKRESVNAIEKRQGGVRAVIVPNDQMQTPHYSVLRVRKGEESSVLSYLLPQIHEAEMLQPSEEMPTERKRPEQPALAAFALQSEAPPTSFERPEAVEQPAQTRPAAVAAPAAAKPGLVSRFFSGLKGLFAAEPQAEAKPVEVAKAEPETTENENRRNGDRRNQRRQGNGRRDRNGERGERGNRYNRSEGRDNNRDNRGEGRDNRENNREQREDQRRNRRNQEPVVAAETRNDEETTASRDEQQQAPRRERGDRSRRRSDDKRKNTQEDKVQEVVAQEVVANVVEETAQEDRQPTQRRQRRQLSQKVRFESEIDHEAEAAAQQLITGIMPTVTPAAQEARAEQDVEQTANAETEEGQNEGGREGGMPRRSRRSPRHLRVSGQRRRRYRDERYPSQSAMPLVGAFASPEMASGKVWISYPVAQAEQQEQHAAVVENAETLTNQVEIAQENTPAPVVTEVVEAPKQPAVETPAAEAPAETVTEHQAPAVEEVAKPAVAEPVASEPQVAAQPVADEPVLVEAETVEPVEPTPVVEVKPEAQQPVLVEPTVEPAIVEPVVAEPEVAEPVVATEPKVEPVQPAAIKAAVEAVATDVAAPRFKHHATAPMTKAPAPDYVPEPARQSDWVRPEFNFDGKGSAGGHAAVTTATAPATKPAMPTE

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias589-720Basic and acidic residues
Compositional bias739-759Basic and acidic residues
Compositional bias805-819Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JMPJ01000053
EMBL· GenBank· DDBJ
KFC80897.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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