A0A084FVC3 · A0A084FVC3_PSEDA
- ProteinKynurenine formamidase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids286 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.
Catalytic activity
- H2O + N-formyl-L-kynurenine = formate + H+ + L-kynurenine
Pathway
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 136 | Nucleophile | ||||
Sequence: S | ||||||
Active site | 230 | |||||
Sequence: D | ||||||
Active site | 262 | |||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | arylformamidase activity | |
Molecular Function | lipase activity | |
Biological Process | 'de novo' NAD biosynthetic process from tryptophan | |
Biological Process | tryptophan catabolic process to kynurenine |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameKynurenine formamidase
- EC number
- Short namesKFA ; KFase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Microascales > Microascaceae > Scedosporium
Accessions
- Primary accessionA0A084FVC3
Proteomes
Organism-specific databases
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 38-268 | AB hydrolase-1 | ||||
Sequence: LIFIHGGAWRDPTNTYQDFLPTINALLSAQSVPPAAIRGFASIDYRLSAHPNHPQDPATPPDKFRSAKHPDHINDVRLALQYLQSKYDIRDRYILIGHSVGATLAFQLLMGQLTTALSSPQIAEPLPAAIVGVAGIYEFYDFVYRHSAPYVTMVEGALGPDHSKWNDDAPLKASYAGLESGGPKVLLAYSPEDSLVDGDRETTAMADHLRADGIDTDVIFIKGEHDFSWQD | ||||||
Motif | 42-46 | HGGXW | ||||
Sequence: HGGAW |
Domain
The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.
Sequence similarities
Belongs to the kynurenine formamidase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length286
- Mass (Da)31,659
- Last updated2014-10-29 v1
- Checksum472FC1F92E684997
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JOWA01000165 EMBL· GenBank· DDBJ | KEZ39035.1 EMBL· GenBank· DDBJ | Genomic DNA |