A0A078KHK4 · A0A078KHK4_9GAMM

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site47L-glutamine (UniProtKB | ChEBI)
Binding site234L-glutamine (UniProtKB | ChEBI)
Binding site236L-glutamine (UniProtKB | ChEBI)
Active site262Nucleophile
Binding site263L-glutamine (UniProtKB | ChEBI)
Binding site266L-glutamine (UniProtKB | ChEBI)
Binding site304L-glutamine (UniProtKB | ChEBI)
Binding site307L-glutamine (UniProtKB | ChEBI)
Active site346
Active site348

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      CEM_008

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Oceanospirillales > Halomonadaceae > Zymobacter group > Candidatus Johnevansia

Accessions

  • Primary accession
    A0A078KHK4

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-185CPSase
Domain3-133Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    373
  • Mass (Da)
    42,124
  • Last updated
    2014-10-29 v1
  • Checksum
    EC3E8E1648A01E65
MIKKAILALEDGNIFHGISIGVDGLISGEIVFNTSMTGYQEILTDPSYLKQIIVFTSSHIGNTGINKEDIESYNIFVSGLIIHDLPSYYSNFRSVMSLKKYLILNNIICISNIDTRKLTRLIRNKNIKNVTILSGEISYKNIKLAITAAKNFHVFNYININNITHDYNHIQLILDKKSINLKNNYNIVYYDLGSKYNIFRILTKLGCKLHIINQNASLSEIIKFNPDGIFLSNGPGDPNDFKNTITVIKELCKKQIPIFGICLGHQLLAIASGAKTKKMKYGHHGSNHPIQDIVTGKLMITSQNHNFIVDEKTLSNKLRVTHRSLFDGTLQGIERIDCPAFSFQGHPEASPGPHDLFLLFSRFISIIKKYKLH

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LM655252
EMBL· GenBank· DDBJ
CDZ16284.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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