A0A077M4H0 · A0A077M4H0_9MICO
- ProteinPhosphoenolpyruvate carboxykinase [GTP]
- GenepckG
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids620 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Catalytic activity
- GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 74 | substrate | ||||
Sequence: R | ||||||
Binding site | 211-213 | substrate | ||||
Sequence: YGG | ||||||
Binding site | 264 | substrate | ||||
Sequence: S | ||||||
Binding site | 265-270 | GTP (UniProtKB | ChEBI) | ||||
Sequence: ASGKTN | ||||||
Active site | 266 | |||||
Sequence: S | ||||||
Binding site | 392-394 | substrate | ||||
Sequence: NSR | ||||||
Binding site | 394 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 425 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 519-522 | GTP (UniProtKB | ChEBI) | ||||
Sequence: YREN |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | GTP binding | |
Molecular Function | kinase activity | |
Molecular Function | manganese ion binding | |
Molecular Function | phosphoenolpyruvate carboxykinase (GTP) activity | |
Biological Process | cellular response to glucose stimulus | |
Biological Process | gluconeogenesis | |
Biological Process | glycerol biosynthetic process from pyruvate | |
Biological Process | propionate catabolic process | |
Biological Process | response to lipid | |
Biological Process | response to starvation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate carboxykinase [GTP]
- EC number
- Short namesPEP carboxykinase ; PEPCK
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Micrococcales > Intrasporangiaceae > Nostocoides
Accessions
- Primary accessionA0A077M4H0
Proteomes
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 20-231 | Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal | ||||
Sequence: EFVRHWAGITGAARVEVVSAADDGRLIEEALAAGEIFPAGEGRYYSRSYPKDTARSEERTIVATSNPADKGVYDNWRPSSEMKPLLERQMSGASAGKTMYVVPYLMAPPGSPLHAYAVGVELTDSRPVVLHMIRMARVGVEHVEGLEDPSMFVRAVHVTGDLPAMQHGTPDDQRYFVTVADERTILHYGSSYGGNALLGKIAHGLRQAAYDG | ||||||
Domain | 238-604 | Phosphoenolpyruvate carboxykinase C-terminal P-loop | ||||
Sequence: LGEQFMLIGIEDRVAGRTWHICGGFPSASGKTNLAMMLPPDALGDRYRVFFYGDDIAWLWVNPDDGRLYGLNPEFGVFGVAKDTNEKTNPTALESVGPGTGAIFTNVAYNPDTQEVWWEGRTPEPPADVAGWLDWKGEPIADRPEGSTEPWSHPNSRFTTTLANVPNIAPDYESPAGVPIDGIIFGGRTRDREPLIRAITDLAEGVYDGLTLGAEATFAAEGVDGQLRYDPMSMRPFMSYPEGPYAAQWLSIVGAASEQPIFAHVNWFQRDPEDGHFLWPGYRENLRPLLWLMRLKDGTVTGRSTPVGILPTEEELDLEGVDITPADLETVLSIDVERWRQEMGFRQAHLEQFDGLPEAIWAAHRRV |
Sequence similarities
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length620
- Mass (Da)68,001
- Last updated2014-10-29 v1
- ChecksumE81CCEDC3FE3E2D0
Keywords
- Technical term