A0A076V6D5 · A0A076V6D5_9VIRU
- ProteinPolyprotein P1234
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids2474 (go to sequence)
- Protein existencePredicted
- Annotation score5/5
Function
function
Inactive precursor of the viral replicase, which is activated by cleavages carried out by the viral protease nsP2.
Catalytic activity
- 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + H2O = L-aspartyl-[protein] + ADP-D-ribose + H+This reaction proceeds in the forward direction.
- 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + H2O = L-glutamyl-[protein] + ADP-D-ribose + H+This reaction proceeds in the forward direction.
- GTP + S-adenosyl-L-methionine = N7-methyl-GTP + S-adenosyl-L-homocysteine
- N7-methyl-GTP + L-histidyl-[protein] = N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] + diphosphateThis reaction proceeds in the forward direction.
- N(tele)-(N7-methylguanosine 5'-phospho)-L-histidyl-[protein] + a 5'-end diphospho-(purine-ribonucleoside) in mRNA + H+ = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleoside) in mRNA + L-histidyl-[protein]
Cofactor
Protein has several cofactor binding sites:
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 1013 | For cysteine protease nsP2 activity | |||
Active site | 1083 | For cysteine protease nsP2 activity | |||
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended namePolyprotein P1234
- Alternative names
Organism names
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Kitrinoviricota > Alsuviricetes > Martellivirales > Togaviridae > Alphavirus
Accessions
- Primary accessionA0A076V6D5
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Host cell membrane ; Lipid-anchor
Host cytoplasmic vesicle membrane ; Lipid-anchor
Host cytoplasmic vesicle membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Interaction
Subunit
Interacts with RNA-directed RNA polymerase nsP4. Interacts with mRNA-capping enzyme nsP1. Interacts with KPNA1/karyopherin-alpha1; this interaction probably allows the active transport of protease nsP2 into the host nucleus.
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 28-259 | Alphavirus-like MT | |||
Domain | 690-991 | +RNA virus helicase C-terminal | |||
Domain | 1004-1327 | Peptidase C9 | |||
Domain | 1324-1493 | Macro | |||
Region | 1692-1712 | Disordered | |||
Region | 1778-1803 | Disordered | |||
Domain | 2228-2343 | RdRp catalytic | |||
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,474
- Mass (Da)275,805
- Last updated2014-10-29 v1
- MD5 ChecksumD64E02540BC81EA1701938A097E84112
Keywords
- Coding sequence diversity
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KC149887 EMBL· GenBank· DDBJ | AIK21918.1 EMBL· GenBank· DDBJ | Other RNA |