A0A076JG35 · A0A076JG35_BIFAD
- ProteinAspartate--tRNA(Asp/Asn) ligase
- GeneaspS
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids599 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).
Catalytic activity
- tRNA(Asx) + L-aspartate + ATP = L-aspartyl-tRNA(Asx) + AMP + diphosphate
Features
Showing features for site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 35 | Important for tRNA non-discrimination | ||||
Sequence: H | ||||||
Site | 81 | Important for tRNA non-discrimination | ||||
Sequence: G | ||||||
Binding site | 180 | L-aspartate (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 226 | L-aspartate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 226-228 | ATP (UniProtKB | ChEBI) | ||||
Sequence: RDE | ||||||
Binding site | 235 | ATP (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 457 | L-aspartate (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 491 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 498 | L-aspartate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 543-546 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GWDR |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | aspartate-tRNA ligase activity | |
Molecular Function | aspartate-tRNA(Asn) ligase activity | |
Molecular Function | ATP binding | |
Molecular Function | nucleic acid binding | |
Biological Process | aspartyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAspartate--tRNA(Asp/Asn) ligase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Bifidobacteriales > Bifidobacteriaceae > Bifidobacterium
Accessions
- Primary accessionA0A076JG35
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 147-564 | Aminoacyl-transfer RNA synthetases class-II family profile | ||||
Sequence: LKLRAQMSKAARHALEEMGFEEIETPTMIKSTPEGARDFVVPARLVPGSWYALPQSPQLLKQLLMVSGVERYYQLARCYRDEDFRADRQPEFTQLDMEMAFVDQEDVMAMAEKVIAAIWKSAGYDIKLPIQRITWQDAMDKYGSDKPDLRFGNPLIELTDYFKNTPFRVFQAPYVGAVLFKGGAATPRRQFDAWQDWAKQRGAKGLAYVVFAENGELKGPVAKNLSEEERNGLKEAVGAEDGDAVFFAAGRRTSSQELLGAVRVELARRAGLLKPDDFAFTWVVDFPLFKPTDDPDDDDVAVGHSKWTSMHHPFTMPSKDWIDKFDKDPEHAMSDSYDIVCNGNEMGGGSVRIHRDDIQDRVLDVLGITPEEAADKFGFLLEAFKYGAPPHAGIALGWDRTAAILAGADSIRDVIAFP | ||||||
Region | 204-207 | Aspartate | ||||
Sequence: QLLK | ||||||
Region | 566-599 | Disordered | ||||
Sequence: AGGGRDPLTGAPAPISAEQRAETGVDYDPDEDED |
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length599
- Mass (Da)66,945
- Last updated2014-10-29 v1
- Checksum3D858AE4CECF1F6E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP028341 EMBL· GenBank· DDBJ | AVT45009.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CYYI01000004 EMBL· GenBank· DDBJ | CUN74947.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BPPZ01000001 EMBL· GenBank· DDBJ | GJD13376.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WDLT01000001 EMBL· GenBank· DDBJ | KAB5748255.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WDIP01000001 EMBL· GenBank· DDBJ | KAB5887255.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WDFR01000001 EMBL· GenBank· DDBJ | KAB6032192.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JANFYM010000001 EMBL· GenBank· DDBJ | MCQ4791875.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MAXD01000007 EMBL· GenBank· DDBJ | OFA34268.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LNKD01000001 EMBL· GenBank· DDBJ | OSG87721.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LNKC01000003 EMBL· GenBank· DDBJ | OSG88994.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LNKF01000002 EMBL· GenBank· DDBJ | OSG95370.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LNKH01000003 EMBL· GenBank· DDBJ | OSG98021.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LNKI01000001 EMBL· GenBank· DDBJ | OSH01573.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP123050 EMBL· GenBank· DDBJ | WGJ18151.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP133648 EMBL· GenBank· DDBJ | WNE85930.1 EMBL· GenBank· DDBJ | Genomic DNA |