A0A076EHF9 · A0A076EHF9_RHOOP

Function

function

Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.

Catalytic activity

Cofactor

biotin (UniProtKB | Rhea| CHEBI:57586 )

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site117ATP (UniProtKB | ChEBI)
Binding site200ATP (UniProtKB | ChEBI)
Binding site235ATP (UniProtKB | ChEBI)
Active site292
Binding site533Mn2+ (UniProtKB | ChEBI)
Binding site605substrate
Binding site703Mn2+ (UniProtKB | ChEBI); via carbamate group
Binding site732Mn2+ (UniProtKB | ChEBI)
Binding site734Mn2+ (UniProtKB | ChEBI)
Binding site867substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionbiotin carboxylase activity
Molecular Functionmetal ion binding
Molecular Functionpyruvate carboxylase activity
Biological Processgluconeogenesis
Biological Processpyruvate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyruvate carboxylase
  • EC number

Gene names

    • ORF names
      EP51_07920

Organism names

Accessions

  • Primary accession
    A0A076EHF9

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue703N6-carboxylysine
Modified residue1093N6-biotinyllysine

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain1-449Biotin carboxylation
Domain121-317ATP-grasp
Domain524-793Pyruvate carboxyltransferase
Region929-950Disordered
Domain1053-1127Lipoyl-binding

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,134
  • Mass (Da)
    121,798
  • Last updated
    2014-10-29 v1
  • Checksum
    56CF87CFA6CDEA56
MFSKVLVANRGEIAIRAFRAAYELGAGTVAVFPYEDRNSIHRLKADESYQIGEEGHPVRAYLSVDEIVSAAKQAGADAIYPGYGFLSENPDLAAACAEAGITFVGPSAEVLELTGNKARAIAAAKAAGLPVLASSEPSADVDELLAAAETMQFPLFVKAVAGGGGRGMRRVAERAQLKESIEAAAREAESAFGDPTVFLEQAVVDPRHIEVQILADGQGNVIHLFERDCSLQRRHQKVIELAPAPNLSEELRAKICADAVAFAKEINYACAGTVEFLLDTRGNHVFIEMNPRIQVEHTVTEEVTDVDLVQSQLRIASGETLADLGLSQDKITLRGAALQCRITTEDPANGFRPDTGRITGYRTPGGAGIRLDGGTSVGAEVSAYFDSMLVKLTCRGRDFETAVARARRAVTEFRIRGVSTNIPFLQAVLDDPDFKAGRVTTSFIEERPQLLTLRSSADRGTKILTYLADVTVNKPHGERPSAVYPRDKLPQIDLSTPPPDGSRQKLLALGPEGFAKALREQKALAVTETTFRDAHQSLLATRVRTSGLLDVAGHVARLTPELLSIEAWGGATYDVALRFLHEDPWYRLAALREAVPNICLQMLLRGRNTVGYTPYPEKVTRAFVEEATNSGIDIFRIFDALNNVDQMRPAIDAVRETGTALAEVALSYTGDLSNPNENLYTLDYYLRLAEEIVEAGAHIIAIKDMAGLLRAPAATTLVTALRKNFDLPVHVHTHDTPGGQLATYLAAWQAGADAVDGASAAMAGTTSQPALSAIVAAAAHSQYDTGLDLQAVCDLEPYWEALRKVYKPFESGLPAPTGRVYTHEIPGGQLSNLRTQAVALGLGDKFEEVEAKYAAADRMLGRLVKVTPSSKVVGDLALHLVGSGASTEEFKENPAKFDIPDSVVGFLRGELGTPPGGWPEPFRTRALEGRGAAKPEVPLSAEDEKALSGTSAERRATLNRLLFPGPTKEFLEHRDKYGDTSQLSANQFFYGLRRGDEHRVRLAPGVELIIGLEAISEPDERGYRTVMCILNGQLRPVSVRDRSISSEIPAAEKADKNNPGHVPAPFAGVVTLAVTEGQHITAGDTIATIEAMKMEAAITAPRSGTVSRLAIGSVQQVEGGDLLAVVSTGESASE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP008947
EMBL· GenBank· DDBJ
AII04523.1
EMBL· GenBank· DDBJ
Genomic DNA

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