A0A076EHF9 · A0A076EHF9_RHOOP
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1134 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- N6-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N6-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H+This reaction proceeds in the forward direction.
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 117 | ATP (UniProtKB | ChEBI) | |||
Binding site | 200 | ATP (UniProtKB | ChEBI) | |||
Binding site | 235 | ATP (UniProtKB | ChEBI) | |||
Active site | 292 | ||||
Binding site | 533 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 605 | substrate | |||
Binding site | 703 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 732 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 734 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 867 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | biotin carboxylase activity | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Nocardiaceae > Rhodococcus
Accessions
- Primary accessionA0A076EHF9
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 703 | N6-carboxylysine | |||
Modified residue | 1093 | N6-biotinyllysine | |||
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-449 | Biotin carboxylation | |||
Domain | 121-317 | ATP-grasp | |||
Domain | 524-793 | Pyruvate carboxyltransferase | |||
Region | 929-950 | Disordered | |||
Domain | 1053-1127 | Lipoyl-binding | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,134
- Mass (Da)121,798
- Last updated2014-10-29 v1
- Checksum56CF87CFA6CDEA56
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP008947 EMBL· GenBank· DDBJ | AII04523.1 EMBL· GenBank· DDBJ | Genomic DNA |