A0A075GAE5 · A0A075GAE5_9EURY
- Protein3-methyl-2-oxobutanoate hydroxymethyltransferase
- GenepanB
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids271 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.
Catalytic activity
- 3-methyl-2-oxobutanoate + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Cofactor biosynthesis; coenzyme A biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 51 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 51-52 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | |||
Binding site | 90 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | |||
Binding site | 90 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 120 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | |||
Binding site | 122 | Mg2+ (UniProtKB | ChEBI) | |||
Active site | 189 | Proton acceptor | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 3-methyl-2-oxobutanoate hydroxymethyltransferase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | methyltransferase activity | |
Biological Process | coenzyme A biosynthetic process | |
Biological Process | methylation | |
Biological Process | pantothenate biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-methyl-2-oxobutanoate hydroxymethyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageArchaea > Euryarchaeota > environmental samples
Accessions
- Primary accessionA0A075GAE5
Subcellular Location
Interaction
Subunit
Homodecamer; pentamer of dimers.
Structure
Sequence
- Sequence statusComplete
- Length271
- Mass (Da)28,924
- Last updated2014-10-29 v1
- MD5 ChecksumF08DD2A205D7D445A7AC066D2E7CE860
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KF900610 EMBL· GenBank· DDBJ | AIF01041.1 EMBL· GenBank· DDBJ | Genomic DNA |