A0A075ECR6 · A0A075ECR6_9ALPC

Function

function

Forms a primer, NSP9-pU, which is utilized by the polymerase for the initiation of RNA chains. Interacts with ribosome signal recognition particle RNA (SRP). Together with NSP8, suppress protein integration into the cell membrane, thereby disrupting host immune defenses.
RNA-directed RNA polymerase that catalyzes the transcription of viral genomic and subgenomic RNAs. Acts in complex with nsp7 and nsp8 to transcribe both the minus and positive strands of genomic RNA. The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the amino terminus of NSP9, forming a covalent RNA-protein intermediate that serves as transcription/replication primer. Subgenomic RNAs (sgRNAs) are formed by discontinuous transcription: The polymerase has the ability to pause at transcription-regulating sequences (TRS) and jump to the leader TRS, resulting in a major deletion. This creates a series of subgenomic RNAs that are replicated, transcribed and translated. In addition, Nsp12 is a subunit of the viral RNA capping enzyme that catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. Subsequently, the NiRAN domain transfers RNA to GDP, and forms the core cap structure GpppA-RNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site5728
Active site5730
Active site5829
Active site5905
Active site5910
Binding site5968-5974S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site6381
Active site6396
Active site6437

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type deubiquitinase activity
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processsymbiont-mediated perturbation of host ubiquitin-like protein modification
Biological Processsymbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity
Biological Processviral genome replication
Biological Processviral protein processing
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Organism names

  • Taxonomic identifier
  • Strain
    • USA/Kansas125/2014
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Alphacoronavirus > Pedacovirus

Accessions

  • Primary accession
    A0A075ECR6

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1964-1982Helical
Transmembrane2025-2044Helical
Transmembrane2104-2125Helical
Transmembrane2132-2153Helical
Transmembrane2165-2185Helical
Transmembrane2528-2546Helical
Transmembrane2787-2804Helical
Transmembrane2859-2887Helical
Transmembrane3337-3354Helical
Transmembrane3361-3381Helical
Transmembrane3401-3419Helical
Transmembrane3431-3449Helical
Transmembrane3469-3493Helical
Transmembrane3500-3520Helical

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain2-109CoV Nsp1 globular
Domain112-364CoV Nsp2 N-terminal
Domain383-776CoV Nsp2 middle
Domain778-895CoV Nsp2 C-terminal
Domain896-991Ubiquitin-like
Region1013-1035Disordered
Domain1057-1296Peptidase C16
Domain1286-1465Macro
Domain1630-1685Ubiquitin-like
Domain1691-1951Peptidase C16
Domain2038-21023Ecto
Region2176-2266Y1
Domain2176-2516CoV Nsp3 Y
Region2180-2193ZF1
Region2226-2236ZF2
Region2267-2516CoV-Y
Region2415-2516Y4
Domain2902-2997Nsp4C
Domain2998-3299Peptidase C30
Domain3580-3662RdRp Nsp7 cofactor
Domain3663-3857RdRp Nsp8 cofactor
Domain3858-3965Nsp9 ssRNA-binding
Domain3966-4103ExoN/MTase coactivator
Domain4120-4369NiRAN
Domain4375-4473Nsp12 Interface
Domain4474-5041Nsp12 RNA-dependent RNA polymerase
Domain5042-5125CV ZBD
Domain5289-5650+RNA virus helicase C-terminal
Domain5710-5924ExoN
Domain5933-6154N7-MTase
Region6045-6059GpppA-binding
Domain6156-6216Nsp15 N-terminal oligomerization
Domain6217-6334AV-Nsp11N/CoV-Nsp15M
Domain6351-6491NendoU
Domain6495-6791Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    6,795
  • Mass (Da)
    754,990
  • Last updated
    2014-10-29 v1
  • Checksum
    3AFCD167A91C04E8

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KJ645701
EMBL· GenBank· DDBJ
AID57062.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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