A0A075DMU8 · A0A075DMU8_9CREN

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site599ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS

Organism names

Accessions

  • Primary accession
    A0A075DMU8

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain17-634Aminoacyl-tRNA synthetase class Ia
Motif596-600'KMSKS' region
Domain683-830Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,047
  • Mass (Da)
    120,665
  • Last updated
    2014-10-29 v1
  • Checksum
    2EC347E0FF815936
MKPLGNKFDLKAIESEIIEFWDKEKVYEKLKEEVSKRSKKFLFIDGPPYPSAPVPHIGTIWNKVIKDCILRFERLNGYKVYDQPGYDTHGLPIEVAVEKQLGITKKQEIIDKIGVGKFVSLCKEFALKNASSMTQNFINIGVFMDWKNPYYTLDNKYISNSWALIKKAHEKGLLEKDVEVLHWCPRCETTLSDYEVSEYRELEDPSIYVKFKVKNEPNKYLVIWTTTPWTLPSNVFVMINKDYDYADVQVGNEVYVIAEKRVKDVMKESGIKEYKVLRVYKGSELLGVQYEHPLKDLVKAQASLDSYHVVVDAGEVVTLEEGTGLVHSAPGHGDVDFEIGKKLGMPVVMLVDDRGNFTEDAGKYKGKYVRDASAEIVEDLKNRKALLHASKIVHRYPVCWRCKSPLILRAIQQWFIRVTKLKTDLLKEIERVNWVPEWGKTRIGNMVKELRDWVISRQRFWGNPLPIWVCSKGHVTVVGSVDELRKIALNEVPEDLHKPWIDEVKVRCGQCGEEAIRVPDVADVWFDSGVAFFASLGDDWEKKWAELSPVDLVLEGHDQLRGWFFSLLRAGVILTGKAPYESVLVHGFMLDEQGREMHKSLGNYVEPSVVIEKYGRDVLRLWLLRNTTWEDAKFSWKSLELTKRDLQIAWNVYVFASMYMSLDNFDPQKYSFSDVRNALKSEDLWILSRFNRLLRDVKESMKNYKVHELANRVMQFIVEDVSRFYLRLVRSRAWVEGDDPSKIAMYYVLYYVLKNWAILASPVIPYTAEKIYRSFVVGGKLSVSTELLPEVDPTLINDELEKAFDLVREISEAGLNARAKAGVKLRWPLKHAYVFLKSRDDINTLSKVKEVLRTTLNVKNVEVMEIDSLHKFSRVVAEPNSGAIGKEFKQLTPKIVSYINENREVVAEDVLEKGSHRAIVDGIETELKISHVNIKEETAEGYAIARFDSGAIAISKELSKEEEEEGLVRDIIRRIQFMRKQLNLNVVDNIVVSIVPPEDKKAIIEKWKDYIKSETRAVEIVMDSAEGDLVNEWEIEEDKYVIGIKKT

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KC912720
EMBL· GenBank· DDBJ
AHM94048.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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