A0A073JQA1 · A0A073JQA1_LIMRT
- ProteinDNA-directed RNA polymerase subunit beta'
- GenerpoC
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1211 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 2 Zn2+ ions per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 60 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 62 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 75 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 78 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 449 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 451 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 453 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 818 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 892 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 899 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 902 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta'
- EC number
- Short namesRNAP subunit beta'
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Lactobacillaceae > Limosilactobacillus
Accessions
- Primary accessionA0A073JQA1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Interaction
Subunit
The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 224-503 | RNA polymerase N-terminal | ||||
Sequence: EWMVMDVIPVMPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLKLQAPGIIVQNEKRMLQEAVDALIDNGRRGRPVAGPGNRPLKSLSHLLKGKQGRFRQNLLGKRVDYSGRSVIDVGPSLKLNQMGLPVPMALELFKPFIMHELVKRGLSANVKAAKRKIDRSDDDVFDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPILVSGKSMRLHPLVCSAYNADFDGDQMAIHVPLSDEAQAEARLLMLAAHHILSPRDGEPIVSPSQDMVIGNYYMT |
Sequence similarities
Belongs to the RNA polymerase beta' chain family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,211
- Mass (Da)135,392
- Last updated2014-10-01 v1
- Checksum48CB810ADA1278FD
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LN887507 EMBL· GenBank· DDBJ | CUR40015.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JOSX01000011 EMBL· GenBank· DDBJ | KEK15885.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAFFPO010000032 EMBL· GenBank· DDBJ | MCH5358040.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WJND01000001 EMBL· GenBank· DDBJ | MRG88452.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WJNC01000005 EMBL· GenBank· DDBJ | MRI07585.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MCNS01000012 EMBL· GenBank· DDBJ | OCX47219.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
NFHN01000009 EMBL· GenBank· DDBJ | OUN49174.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
NFKV01000011 EMBL· GenBank· DDBJ | OUP89288.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QAZN01000011 EMBL· GenBank· DDBJ | PTV03670.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP041676 EMBL· GenBank· DDBJ | QDR71998.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP059275 EMBL· GenBank· DDBJ | QLQ62699.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
PTLS01000022 EMBL· GenBank· DDBJ | RMX25764.1 EMBL· GenBank· DDBJ | Genomic DNA |