A0A072V223 · A0A072V223_MEDTR
- ProteinATP-dependent 6-phosphofructokinase
- Gene25490151
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids519 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H+
Cofactor
Activity regulation
Allosterically activated by AMP.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 102 | ATP (UniProtKB | ChEBI) | |||
Binding site | 165-166 | ATP (UniProtKB | ChEBI) | |||
Binding site | 190-193 | ATP (UniProtKB | ChEBI) | |||
Binding site | 191 | Mg2+ (UniProtKB | ChEBI); catalytic | |||
Site | 192 | Important for substrate specificity; cannot use PPi as phosphoryl donor | |||
Binding site | 219-221 | substrate | |||
Active site | 221 | Proton acceptor | |||
Binding site | 264-266 | substrate | |||
Binding site | 320 | substrate | |||
Binding site | 377-380 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent 6-phosphofructokinase
- EC number
- Short namesATP-PFK ; Phosphofructokinase
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Trifolieae > Medicago
Accessions
- Primary accessionA0A072V223
Proteomes
Genome annotation databases
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 96-401 | Phosphofructokinase | |||
Region | 470-519 | Disordered | |||
Compositional bias | 499-519 | Basic and acidic residues | |||
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length519
- Mass (Da)56,835
- Last updated2014-10-01 v1
- ChecksumA1E010E9943EB73E
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 499-519 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM001219 EMBL· GenBank· DDBJ | KEH36099.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
PSQE01000003 EMBL· GenBank· DDBJ | RHN70911.1 EMBL· GenBank· DDBJ | Genomic DNA |