A0A068V2G5 · A0A068V2G5_COFCA

Function

function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N6-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site107[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site112[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site118[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site138[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site142[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site145[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site354[4Fe-4S] cluster 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmitochondrion
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionlipoate synthase activity
Molecular Functionmetal ion binding
Biological Processprotein lipoylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lipoyl synthase, mitochondrial
  • EC number
  • Alternative names
    • Lipoate synthase
      (LS
      ; Lip-syn
      )
    • Lipoic acid synthase

Gene names

    • Name
      LIP1
    • ORF names
      GSCOC_T00042166001

Organism names

  • Taxonomic identifier
  • Strain
    • cv. DH200-94
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Gentianales > Rubiaceae > Ixoroideae > Gardenieae complex > Bertiereae - Coffeeae clade > Coffeeae > Coffea

Accessions

  • Primary accession
    A0A068V2G5

Proteomes

Genome annotation databases

Subcellular Location

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain123-343Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    375
  • Mass (Da)
    41,582
  • Last updated
    2014-10-01 v1
  • Checksum
    D2B96951AA0514E7
MNSRLTGLFTRSLKATKFFSTLNPSLPPQPSPQFARTLEGLRQRLAEESPTLADFIKLQSENEYSVEVGTKKKPLPKPKWMKESIPGGEKYTHIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKALKPNMLIEALVPDFRGDPSCVEKVATSGLDVFAHNIETVEELQSVVRDHRANFEQSMDVLMKAKDYAPVGTLTKTSIMLGCGETPDQVVKTMEKVRAAGVDVMTFGQYMRPSKRHMSVSEYITPEAFEKYRVLGMEMGFRYVASGPMVRSSYKAGEYYIKSMIQSDRAASSS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HG739175
EMBL· GenBank· DDBJ
CDP14732.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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