A0A066RIA0 · A0A066RIA0_ECOLX
- ProteinBifunctional NAD(P)H-hydrate repair enzyme
- Genennr
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids510 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.
Catalytic activity
- (6R)-NADHX = (6S)-NADHX
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 potassium ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 66-70 | (6S)-NADPHX (UniProtKB | ChEBI) | |||
Binding site | 67 | K+ (UniProtKB | ChEBI) | |||
Binding site | 130 | K+ (UniProtKB | ChEBI) | |||
Binding site | 134-140 | (6S)-NADPHX (UniProtKB | ChEBI) | |||
Binding site | 163 | (6S)-NADPHX (UniProtKB | ChEBI) | |||
Binding site | 166 | K+ (UniProtKB | ChEBI) | |||
Binding site | 264 | (6S)-NADPHX (UniProtKB | ChEBI) | |||
Binding site | 324 | (6S)-NADPHX (UniProtKB | ChEBI) | |||
Binding site | 370 | (6S)-NADPHX (UniProtKB | ChEBI) | |||
Binding site | 407-411 | AMP (UniProtKB | ChEBI) | |||
Binding site | 436 | AMP (UniProtKB | ChEBI) | |||
Binding site | 437 | (6S)-NADPHX (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ADP-dependent NAD(P)H-hydrate dehydratase activity | |
Molecular Function | ATP binding | |
Molecular Function | kinase activity | |
Molecular Function | metal ion binding | |
Molecular Function | NAD(P)HX epimerase activity | |
Biological Process | metabolite repair | |
Biological Process | nicotinamide nucleotide metabolic process |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Recommended nameBifunctional NAD(P)H-hydrate repair enzyme
- Alternative names
Including 2 domains:
- Recommended nameADP-dependent (S)-NAD(P)H-hydrate dehydratase
- EC number
- Alternative names
- Recommended nameNAD(P)H-hydrate epimerase
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionA0A066RIA0
Proteomes
Interaction
Subunit
Homotetramer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 18-220 | YjeF N-terminal | |||
Domain | 229-496 | YjeF C-terminal | |||
Sequence similarities
Belongs to the NnrD/CARKD family.
Belongs to the NnrE/AIBP family.
In the C-terminal section; belongs to the NnrD/CARKD family.
In the N-terminal section; belongs to the NnrE/AIBP family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length510
- Mass (Da)53,927
- Last updated2014-09-03 v1
- MD5 Checksum3BBDCE8A6BBA6F38C5B5F5C94848F63C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP018802 EMBL· GenBank· DDBJ | BBF56460.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MOHC01000021 EMBL· GenBank· DDBJ | OJN37231.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UGCD01000002 EMBL· GenBank· DDBJ | STI20225.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UGCP01000002 EMBL· GenBank· DDBJ | STI83041.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UGEB01000001 EMBL· GenBank· DDBJ | STK56954.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UGFE01000002 EMBL· GenBank· DDBJ | STM26167.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UGFO01000006 EMBL· GenBank· DDBJ | STN11793.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UWXJ01000001 EMBL· GenBank· DDBJ | VCY81548.1 EMBL· GenBank· DDBJ | Genomic DNA |