A0A062WNJ5 · A0A062WNJ5_9ACTN

  • Protein
    Biotin synthase
  • Gene
    bioB
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site71[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site75[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site78[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site115[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site148[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site208[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site278[2Fe-2S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionbiotin synthase activity
Molecular Functioniron ion binding
Biological Processbiotin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Biotin synthase
  • EC number

Gene names

    • Name
      bioB
    • ORF names
      BMG523Draft_03731

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • BMG5.23
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Frankiales > Frankiaceae > Frankia

Accessions

  • Primary accession
    A0A062WNJ5

Proteomes

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain56-283Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    363
  • Mass (Da)
    38,606
  • Last updated
    2014-09-03 v1
  • Checksum
    E0051CB21DD826C2
MTDMDLSATLNSLVSKGVSGQAPTRDEALAVLRSDDDDLLDVVAAAYRLRRRYFGRRVKLNFLVNLKSGLCPEDCSYCSQRLGSNTGILKYTWLKPEEAAATAGAGISGGARRVCLVASGRGPTDRDVDRVADTIGAIKTAHPDVEVCACLGLLSDGQAAQLRAAGADAYNHNLNTAGEKYADICTTHTYNDRVDTVQEARHAGLSPCSGIIAGMGESDEDLVDVAFALRELAPDSIPVNFLMPFEGTPLGAEWNLNPRQCLRILAMVRFVNPTAEVRLSGGREIHLGSMQPLALSVVNSIFLGDYLTSEGQEGHQDLKMIAEAGFTVEGLNTDAEAALAMGAGLERVALRQRGAGTDLPPNA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JDWE01000048
EMBL· GenBank· DDBJ
KDA41472.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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