A0A061IIZ4 · A0A061IIZ4_CRIGR

Function

function

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.
    EC:5.6.1.1 (UniProtKB | ENZYME | Rhea)

Activity regulation

ATPase activity is stimulated by microtubules, which promote homooligomerization. ATP-dependent microtubule severing is stimulated by interaction with KATNB1.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site264-271ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcentrosome
Cellular Componentcytoplasm
Cellular Componentmicrotubule
Cellular Componentmidbody
Cellular Componentspindle pole
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functionisomerase activity
Molecular Functionmicrotubule binding
Molecular Functionmicrotubule severing ATPase activity
Biological Processcell division
Biological Processmicrotubule severing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Katanin p60 ATPase-containing subunit A1
  • EC number
  • Short names
    Katanin p60 subunit A1
  • Alternative names
    • p60 katanin

Gene names

    • Name
      KATNA1
    • ORF names
      H671_2g5637

Organism names

Accessions

  • Primary accession
    A0A061IIZ4

Proteomes

Subcellular Location

Cytoplasm
Midbody
Note: Predominantly cytoplasmic. Localized diffusely in the cytoplasm during the interphase. During metaphase is localized throughout the cell and more widely dispersed than the microtubules. In anaphase and telophase is localized at the midbody region. Also localized to the interphase centrosome and the mitotic spindle poles. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1. Localizes within the cytoplasm, partially overlapping with microtubules, in interphase and to the mitotic spindle and spindle poles during mitosis.

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue51Phosphoserine; by DYRK2
Modified residue118Phosphoserine; by DYRK2

Post-translational modification

Phosphorylation by DYRK2 triggers ubiquitination and subsequent degradation.
Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex, leading to its proteasomal degradation. Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted for proteasomal degradation.

Keywords

Interaction

Subunit

Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with KATNB1, which may serve as a targeting subunit. Interacts with ASPM; the katanin complex formation KATNA1:KATNB1 is required for the association of ASPM. Interacts with dynein and NDEL1. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and DCAF1 proteins (EDVP complex). Interacts with KLHL42 (via the kelch domains). Interacts with CUL3; the interaction is enhanced by KLHL42. Interacts with KATNB1 and KATNBL1. Interacts with CAMSAP2 and CAMSAP3; leading to regulate the length of CAMSAP-decorated microtubule stretches.

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region106-197Disordered
Compositional bias117-131Basic and acidic residues
Compositional bias150-182Basic and acidic residues
Domain256-398AAA+ ATPase

Domain

The N-terminus is sufficient for interaction with microtubules, although high affinity binding to microtubules also requires an intact C-terminal domain and ATP, which promotes oligomerization.

Sequence similarities

Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    506
  • Mass (Da)
    57,561
  • Last updated
    2014-09-03 v1
  • Checksum
    69019816C41F0487
MMAQCLHVSMSLLMISENVKLAREYALLGNYDSAMVYYQGVLDQMNKYLYSVKDTYLQQKWQQVWQEINVEAKHVKDIMKTLESFKLDSTPLKAAQHELPASEGEVWSLPVPVERRPSPGPRKRQSSQYHDPKPHSNRPGTGVRAHRPSAQSLHNDRGKAVRSREKKEQSKGREEKKRALDPNKSPAAVTEQEANKFDSTGYDKDLVEALERDIISQNPNVRWDDIADLVEAKKLLKEAVVLPMWMPEFFKGIRRPWKGVLMVGPPGTGKTLLAKAVATECKTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYSPATIFIDEIDSICSRRGTSEEHEASRRVKAELLVQMDGVGGASENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPSAKGREELLRISLRELELADDVNLAIIAENMEGYSGADITNVCRDASLMAMRRRIEGLTPEEIRNLSREEMHMPTTMEDFEMALKKVSKSVSAADIERYEKWIVEFGSC

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias117-131Basic and acidic residues
Compositional bias150-182Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KE667778
EMBL· GenBank· DDBJ
ERE84880.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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