A0A061AE05 · PAPSH_CAEEL
- ProteinBifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase pps-1
- Genepps-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids654 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway (PubMed:16497669).
The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase) (PubMed:16497669).
Required for normal growth and development (PubMed:16497669).
Involved in several aspects of both embryonic and postembryonic development, including molting, changes in cell shape, and patterning of epithelial and muscle cells (PubMed:16497669).
The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase) (PubMed:16497669).
Required for normal growth and development (PubMed:16497669).
Involved in several aspects of both embryonic and postembryonic development, including molting, changes in cell shape, and patterning of epithelial and muscle cells (PubMed:16497669).
Catalytic activity
- sulfate + ATP + H+ = adenosine 5'-phosphosulfate + diphosphate
Pathway
Sulfur metabolism; sulfate assimilation.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 66-71 | ATP 1 (UniProtKB | ChEBI) | |||
Binding site | 93-96 | adenosine 5'-phosphosulfate (UniProtKB | ChEBI) | |||
Binding site | 105 | adenosine 5'-phosphosulfate (UniProtKB | ChEBI) | |||
Binding site | 110-113 | adenosine 5'-phosphosulfate (UniProtKB | ChEBI) | |||
Binding site | 136-137 | adenosine 5'-phosphosulfate (UniProtKB | ChEBI) | |||
Binding site | 175 | adenosine 5'-phosphosulfate (UniProtKB | ChEBI) | |||
Binding site | 190-191 | adenosine 5'-phosphosulfate (UniProtKB | ChEBI) | |||
Binding site | 218 | ATP 1 (UniProtKB | ChEBI) | |||
Binding site | 449-452 | ATP 2 (UniProtKB | ChEBI) | |||
Binding site | 550-554 | ATP 2 (UniProtKB | ChEBI) | |||
Binding site | 592 | ATP 2 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | adenylylsulfate kinase activity | |
Molecular Function | ATP binding | |
Molecular Function | sulfate adenylyltransferase (ATP) activity | |
Biological Process | 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process | |
Biological Process | phosphorylation | |
Biological Process | sulfate assimilation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase pps-1
Including 2 domains:
- Recommended nameSulfate adenylyltransferase
- EC number
- Recommended nameAdenylyl-sulfate kinase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionA0A061AE05
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Lethal phenotype (PubMed:16497669).
Embryos hatch, initiate growth and development, but arrest at the L2 or L3 larval stages (PubMed:16497669).
Abnormal cuticle (PubMed:16497669).
RNAi-mediated knockdown causes a 42% decrease in 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthesis activity (PubMed:16497669).
Reduced sulfation of disaccharides of heparan sulfate (HS) proteoglycans (PubMed:16497669).
Defects in cell shape of epidermis and patterning of body wall muscle cells (PubMed:16497669).
Some phenotypes only observed when grown at 25 degrees Celsius; such as shortened malformed tail, exacerbated molting defects, blistering, and sterility (PubMed:16497669).
Embryos hatch, initiate growth and development, but arrest at the L2 or L3 larval stages (PubMed:16497669).
Abnormal cuticle (PubMed:16497669).
RNAi-mediated knockdown causes a 42% decrease in 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthesis activity (PubMed:16497669).
Reduced sulfation of disaccharides of heparan sulfate (HS) proteoglycans (PubMed:16497669).
Defects in cell shape of epidermis and patterning of body wall muscle cells (PubMed:16497669).
Some phenotypes only observed when grown at 25 degrees Celsius; such as shortened malformed tail, exacerbated molting defects, blistering, and sterility (PubMed:16497669).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000457177 | 1-654 | Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase pps-1 | ||
Proteomic databases
Expression
Developmental stage
Widely expressed in epidermal cells, gland cells and in amphid sheath cells throughout development, but not in muscle cells and neurons.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-26 | Disordered | |||
Region | 1-231 | Adenylyl-sulfate kinase | |||
Region | 242-653 | Sulfate adenylyltransferase | |||
Sequence similarities
In the N-terminal section; belongs to the APS kinase family.
In the C-terminal section; belongs to the sulfate adenylyltransferase family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
A0A061AE05-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Nameb
- Length654
- Mass (Da)73,172
- Last updated2014-09-03 v1
- ChecksumF5034E4C96E83556
A0A061AE05-2
- Namea
- Differences from canonical
- 176-178: PSE → Q
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Alternative sequence | VSP_061753 | 176-178 | in isoform a | ||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX284604 EMBL· GenBank· DDBJ | CAA93098.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284604 EMBL· GenBank· DDBJ | CDR32729.1 EMBL· GenBank· DDBJ | Genomic DNA |