A0A060VEI2 · A0A060VEI2_KLEPN

  • Protein
    RecBCD enzyme subunit RecB
  • Gene
    recB
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA.

Miscellaneous

In the RecBCD complex, RecB has a slow 3'-5' helicase, an exonuclease activity and loads RecA onto ssDNA, RecD has a fast 5'-3' helicase activity, while RecC stimulates the ATPase and processivity of the RecB helicase and contributes to recognition of the Chi site.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site23-30ATP (UniProtKB | ChEBI)
Binding site953Mg2+ (UniProtKB | ChEBI)
Binding site1064Mg2+ (UniProtKB | ChEBI)
Active site1077For nuclease activity
Binding site1077Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentexodeoxyribonuclease V complex
Molecular Function3'-5' DNA helicase activity
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular Functionexodeoxyribonuclease V activity
Molecular Functionisomerase activity
Molecular Functionmagnesium ion binding
Biological Processdouble-strand break repair via homologous recombination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RecBCD enzyme subunit RecB
  • EC number
  • Alternative names
    • DNA 3'-5' helicase subunit RecB
    • Exonuclease V subunit RecB
      (ExoV subunit RecB
      )
    • Helicase/nuclease RecBCD subunit RecB

Gene names

    • Name
      recB
    • ORF names
      B5L96_03585
      , GJJ13_004465
      , QIG75_02180
      , SAMEA3512100_02402
      , SAMEA3531867_02563
      , SAMEA3649615_02629
      , SAMEA3727706_00281
      , SAMEA4364603_01010
      , SAMEA4370542_01146
      , SAMEA4873632_03212

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • 39383
    • 4300STDY6470422
    • 5012STDY7626430
    • EuSCAPE_BE110
    • EuSCAPE_DE072
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Klebsiella/Raoultella group > Klebsiella

Accessions

  • Primary accession
    A0A060VEI2
  • Secondary accessions
    • A0A4V6LY54

Proteomes

Subcellular Location

Interaction

Subunit

Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding. Interacts with RecA.

Family & Domains

Features

Showing features for region, domain, coiled coil.

TypeIDPosition(s)Description
Region1-844DNA-binding and helicase activity, interacts with RecC
Domain2-450UvrD-like helicase ATP-binding
Domain480-746UvrD-like helicase C-terminal
Coiled coil782-809
Region897-1178Nuclease activity, interacts with RecD and RecA

Domain

The C-terminal domain has nuclease activity and interacts with RecD. It interacts with RecA, facilitating its loading onto ssDNA.
The N-terminal DNA-binding domain is a ssDNA-dependent ATPase and has ATP-dependent 3'-5' helicase function. This domain interacts with RecC.

Sequence similarities

Belongs to the helicase family. UvrD subfamily.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,178
  • Mass (Da)
    132,874
  • Last updated
    2014-09-03 v1
  • Checksum
    7F6AB8858C3A7161
MTDTAESLDPLRLPLIGERLIEASAGTGKTFTIAALYLRLLLGLGGEAAYPRAISVEELLVVTFTEAATEELRGRIRSNIHELRIACLRGESDNPLYSALLAEIADKDDAAKTLLLAERQMDEAAVFTIHGFCQRMLSLNAFESGMLFEQQLIEDESRLRYQACADFWRRHCYPLTRDIAAVIHDVWKGPRDLLKSLDRWLQGEAPQLKSPPAPNETLAERHQQIIARIDSLKQQWREQVGEIEGVLENSGLDRRKFNRGNQGKWMEKVNAWAQEETLSYQLPDALEKFAQSFLLERTKAGGEPPVHPLFSAVESLLASSLTLTDLVLARAMVEIRDAVAREKRRRGELGFDDMLSRLDEALRGDSGETLASAIRQRFPVAMIDEFQDTDPQQYRIFRRIWRRQPETALLLIGDPKQAIYAFRGADIFTYMKARGDVAAHYTLDTNWRSSPGMVGSVNRLFSLSDNPFMFHEIPFLPVKAAAKNKGLRFTVDAADVPAMNVWLMPGDTVGSGDYQTFMAQLCATQIRDWLSAGQQGRALLWRGETSRPVQASDITVLVRNRLEAAQVREALQTLGIPSVYLSNRDSVFETLEAQELLWLLQAVLAPERENTLRSALATSMFGLTALDIENLNQDEQAWDALVEEFSEYRQIWRQRGVMPMLRALMTARHIAENLLATRGGERRLTDILHISELLQEAASQLESEHALVRWLAQHIAEPDSNAASQQMRLESDKHLVQIVTIHKSKGLEYPLVWLPFIARFRKQDQAFYHDRETFAAVLDLGQDEASLELAEAERLAEDLRLLYVALTRAVWHCSLGVAPLSSRKSGNSDFHLSALGRLLQAGEAMDAAGLAARLADFCHGDIALQIPGELDLTPWQAPAATIPRLSARELQRRIADDWRVTSYSGLQQHGFSGGQDLLPRLDVDAAGVGEVVEEPQLTPHQFPRGAAPGTFLHSLFEELDFTQPVPEGWMAEKLQLSGFDAQWAPVLTDWLGGVLKTRLPGPDIALNQLAARDKQVEMAFYLPIAQLLTAERLDALIRQYDPLSADTPPLDFRQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGEDREAYTRPAMEQAMRAHRYDLQYQLYSLALHRYLRHRLADYDYDRHFGGVIYLFLRGMDGQEGGQGIFTTRPVRPLIDGLDQLFAGETQEEAS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JARYWG010000002
EMBL· GenBank· DDBJ
MDH8287411.1
EMBL· GenBank· DDBJ
Genomic DNA
NDBK01000017
EMBL· GenBank· DDBJ
OVF77140.1
EMBL· GenBank· DDBJ
Genomic DNA
CP063023
EMBL· GenBank· DDBJ
QOU79603.1
EMBL· GenBank· DDBJ
Genomic DNA
FWJM01000003
EMBL· GenBank· DDBJ
SLS19157.1
EMBL· GenBank· DDBJ
Genomic DNA
UFEU01000002
EMBL· GenBank· DDBJ
SSK22687.1
EMBL· GenBank· DDBJ
Genomic DNA
UJTF01000001
EMBL· GenBank· DDBJ
SWV71624.1
EMBL· GenBank· DDBJ
Genomic DNA
UKLK01000004
EMBL· GenBank· DDBJ
SXU32910.1
EMBL· GenBank· DDBJ
Genomic DNA
ULET01000005
EMBL· GenBank· DDBJ
SYU71173.1
EMBL· GenBank· DDBJ
Genomic DNA
CAAHCC010000005
EMBL· GenBank· DDBJ
VGK96966.1
EMBL· GenBank· DDBJ
Genomic DNA
FLXF02000002
EMBL· GenBank· DDBJ
VTT40919.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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