A0A060UPI5 · A0A060UPI5_9PROT

Function

function

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site18-20FMN (UniProtKB | ChEBI)
Binding site72FMN (UniProtKB | ChEBI)
Site99Interacts with tRNA
Active site102Proton donor
Binding site140FMN (UniProtKB | ChEBI)
Binding site172FMN (UniProtKB | ChEBI)
Site187Interacts with tRNA
Binding site212-214FMN (UniProtKB | ChEBI)
Binding site234-235FMN (UniProtKB | ChEBI)
Site301Interacts with tRNA; defines subfamily-specific binding signature

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA-dihydrouridine20 synthase activity
Molecular FunctiontRNA-dihydrouridine20a synthase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-dihydrouridine(20/20a) synthase
  • EC number
  • Alternative names
    • U20-specific dihydrouridine synthase
      (U20-specific Dus
      )
    • tRNA-dihydrouridine synthase A

Gene names

    • Name
      yjbN
    • Synonyms
      dusA
    • ORF names
      AFERRI_40153

Organism names

  • Taxonomic identifier
  • Strain
    • CF27
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Acidithiobacillia > Acidithiobacillales > Acidithiobacillaceae > Acidithiobacillus

Accessions

  • Primary accession
    A0A060UPI5

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain16-317DUS-like FMN-binding

Sequence similarities

Belongs to the Dus family. DusA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    359
  • Mass (Da)
    39,667
  • Last updated
    2014-09-03 v1
  • Checksum
    C1D7952C0BF5A550
MTELNKHVAMDKILSVAPMLDWTDRHCRYFLRLVCPSCVLYTEMVTTSALLLGRDPERFLEFSAAEHPLILQLGGDDPAAMRAAAAMAHAYGYDGINLNVGCPSPRVQKGRFGACLMTTPELVAELVAAMGESGLPISVKHRLGLDREEDYATLRAFIETVAMVGCRHFIVHARNAWLKGLSPAENRDVPPLRWDWVHQLKKELPHLTIEINGGFNDLTVVTAQFSAVDGVMIGRSAYHHPLLLAEIERAMGRRETLPVPAVILSGLIEYAEQWGEALPAPRLSRHLHGLRFGQNGAGAWRRFLGTAETGESAAEFLRRGMSLFDWKSGEDVVAHGQQTQSALIADAKVHTTGYPSHQY

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CCCS020000034
EMBL· GenBank· DDBJ
CDQ10201.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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