A0A060IPE9 · A0A060IPE9_9REOV
- ProteinNon-structural protein 1
- GeneNSP1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids493 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Plays a role in the inhibition of host innate immunity by inducing the degradation of key host factors required to activate interferon production such as IRF3, IRF5 or IRF7. Associates with components of cullin RING ligases (CRLs) including CUL1 or CUL3, which are essential multisubunit ubiquitination complexes, to modulate their activities. Recognizes the host NF-kappa-B regulator BTRC through the presence of a DSGXS motif in the C-terminal substrate recognition domain.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | host cell cytoplasm | |
Cellular Component | host cytoskeleton | |
Molecular Function | metal ion binding | |
Molecular Function | RNA binding | |
Biological Process | symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity | |
Biological Process | symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity | |
Biological Process | symbiont-mediated suppression of host NF-kappaB cascade | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameNon-structural protein 1
- Short namesNSP1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Duplornaviricota > Resentoviricetes > Reovirales > Sedoreoviridae > Rotavirus
Accessions
- Primary accessionA0A060IPE9
Proteomes
Subcellular Location
PTM/Processing
Post-translational modification
The C-terminal region is phosphorylated by host CKII/CSNK2A1. Phosphorylation of the DSGXS motif is essential for host NF-kappa-B inhibition.
Keywords
- PTM
Interaction
Subunit
Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation. Interacts with host CUL1 and CUL3. Interacts with host BTRC.
Structure
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 8-88 | RNA-binding | ||||
Sequence: MATFKDACYYYKRINKLNHAVLKLGVNDTWRPSPPTKYKGWCLDCCQHTDLTYCRGCTMYHVCQWCSQYGRCFLDNEPHLL | ||||||
Region | 49-86 | Zinc-binding domain | ||||
Sequence: CLDCCQHTDLTYCRGCTMYHVCQWCSQYGRCFLDNEPH | ||||||
Region | 324-493 | Interaction with host IRF3 | ||||
Sequence: EVHNCKWCSINSSYTVWNDFRVKKIYDNIFNFLRALVKSNVNVGHCSSQEKIYECVENILDVCDNEKWKTSVTKIFNYLEPVELNAVNYVLFNHEVNWDVINVLVQSIGKVPQILTLNDVTTIMQSIIYEWFDTKYMRNTPMTTFTVDKLRRLCTGSKTVDYDSGISDVE | ||||||
Motif | 486-490 | IKBKB-like degron (ILD) motif | ||||
Sequence: DSGIS |
Domain
The integrity of the zinc-binding domain in NSP1 is important for degradation of host IRF3.
Sequence similarities
Belongs to the rotavirus NSP1 family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length493
- Mass (Da)57,963
- Last updated2014-09-03 v1
- ChecksumCB4C4A37A39AC414