A0A031HJ80 · A0A031HJ80_9SPHN

  • Protein
    Bifunctional enzyme IspD/IspF
  • Gene
    ispD
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

a divalent metal cation (UniProtKB | Rhea| CHEBI:60240 )

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Features

Showing features for site, binding site.

TypeIDPosition(s)Description
Site19Transition state stabilizer
Site26Transition state stabilizer
Site144Positions MEP for the nucleophilic attack
Site199Positions MEP for the nucleophilic attack
Binding site224a divalent metal cation (UniProtKB | ChEBI)
Binding site224-2264-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site226a divalent metal cation (UniProtKB | ChEBI)
Site250Transition state stabilizer
Binding site250-2514-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site258a divalent metal cation (UniProtKB | ChEBI)
Binding site272-2744-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site348-3514-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Site349Transition state stabilizer
Binding site3554-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)
Binding site3584-CDP-2-C-methyl-D-erythritol 2-phosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
Molecular Function2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
Molecular Functionmetal ion binding
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional enzyme IspD/IspF

Including 2 domains:

  • Recommended name
    2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
  • EC number
  • Alternative names
    • 4-diphosphocytidyl-2C-methyl-D-erythritol synthase
    • MEP cytidylyltransferase
      (MCT
      )
  • Recommended name
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
  • EC number
  • Short names
    MECDP-synthase
    ; MECPP-synthase
    ; MECPS

Gene names

    • Name
      ispD
    • Synonyms
      ispDF
    • ORF names
      BW41_03766

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • RIT328
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Sphingomonadaceae > Sphingomonas

Accessions

  • Primary accession
    A0A031HJ80

Proteomes

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-2182-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Region218-3742-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Domain219-3702-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Sequence similarities

Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.
Belongs to the IspF family.
In the C-terminal section; belongs to the IspF family.
In the N-terminal section; belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    374
  • Mass (Da)
    38,806
  • Last updated
    2014-07-09 v1
  • Checksum
    E81B5649E43024AC
MTERAYTTALIVAAGSGVRAGGALPKQYATIAGKPLIAHAYAAFADHPAIDRVVVVIAPGAEPLAQAALGAVTLVTGGASRRESVAKGLAAVTGARVLVHDAARPFVPAAVIDRVVAALDAADGVIPAIAVADTLVRDGTVVPRDGLSRVQTPQGFRTEALRAAHAAWPADDEATDDAQMVQRLGGSVALVQGDAMLDKVTHPEDFAAAEARVQWESRSASGFDVHRLEAGEQLWLGGVLIPHDKGLSGHSDADVALHALTDALLGTIAAGDIGTHFPPSDPQWKGADSAQFLQHAATLIAERRGRIDFVDLTLICEAPKIGPHRAVMRERIAALLDLSIDRVSIKATTTERLGFTGRGEGIAAQAVATVSLPR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JFYV01000054
EMBL· GenBank· DDBJ
EZP49098.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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