A0A024L2A9 · A0A024L2A9_ECOLX
- ProteinProtein/nucleic acid deglycase HchA
- GenehchA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids283 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Plays an important role in protecting cells from carbonyl stress.
Catalytic activity
- H2O + N2-(1-hydroxy-2-oxoethyl)-GDP = GDP + glycolate + H+
- H2O + N2-(1-hydroxy-2-oxoethyl)-GMP = glycolate + GMP + H+
- H2O + N2-(1-hydroxy-2-oxoethyl)-GTP = glycolate + GTP + H+
- H2O + N2-(1-hydroxy-2-oxoethyl)-dGTP = dGTP + glycolate + H+
- H2O + N2-(1-hydroxy-2-oxopropyl)-GDP = GDP + H+ + lactate
- H2O + N2-(1-hydroxy-2-oxopropyl)-GMP = GMP + H+ + lactate
- H2O + N2-(1-hydroxy-2-oxopropyl)-GTP = GTP + H+ + lactate
- H2O + N2-(1-hydroxy-2-oxopropyl)-dGTP = dGTP + H+ + lactate
- H2O + N6-(1-hydroxy-2-oxoethyl)-L-lysyl-[protein] = glycolate + H+ + L-lysyl-[protein]
- H2O + N(omega)-(1-hydroxy-2-oxoethyl)-L-arginyl-[protein] = glycolate + H+ + L-arginyl-[protein]
- H2O + S-(1-hydroxy-2-oxoethyl)-L-cysteinyl-[protein] = glycolate + H+ + L-cysteinyl-[protein]
- an N2-(1-hydroxy-2-oxoethyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + glycolate + H+
- an N2-(1-hydroxy-2-oxoethyl)-guanosine in RNA + H2O = a guanosine in RNA + glycolate + H+
- an N2-(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + H+ + lactate
- an N2-(1-hydroxy-2-oxopropyl)-guanosine in RNA + H2O = a guanosine in RNA + H+ + lactate
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | lyase activity | |
Molecular Function | protein deglycase activity | |
Molecular Function | thiolester hydrolase activity | |
Molecular Function | zinc ion binding | |
Biological Process | DNA repair | |
Biological Process | protein repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein/nucleic acid deglycase HchA
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionA0A024L2A9
- Secondary accessions
Proteomes
Subcellular Location
Expression
Induction
By heat shock.
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Sequence similarities
Belongs to the peptidase C56 family. HSP31-like subfamily.
Belongs to the peptidase C56 family. HchA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length283
- Mass (Da)31,176
- Last updated2014-07-09 v1
- Checksum5CA8BE99F9A83B72
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP026399 EMBL· GenBank· DDBJ | AUY00460.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AASLUY010000028 EMBL· GenBank· DDBJ | EFE0301295.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AASQZC010000089 EMBL· GenBank· DDBJ | EFF7861581.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AASWKH010000034 EMBL· GenBank· DDBJ | EFH6097615.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AASXRC010000013 EMBL· GenBank· DDBJ | EFI0213629.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AATGUC010000026 EMBL· GenBank· DDBJ | EFL0834603.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AATHUK010000024 EMBL· GenBank· DDBJ | EFL4330783.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AATJOC010000010 EMBL· GenBank· DDBJ | EFM0253629.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BFIH01000036 EMBL· GenBank· DDBJ | GCO24801.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BFXY01000023 EMBL· GenBank· DDBJ | GDH29133.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BGDN01000132 EMBL· GenBank· DDBJ | GDP81692.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DABALL010000007 EMBL· GenBank· DDBJ | HAH1418325.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DABCAF010000018 EMBL· GenBank· DDBJ | HAH6602868.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DABDSA010000017 EMBL· GenBank· DDBJ | HAI2142651.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DABERK010000007 EMBL· GenBank· DDBJ | HAI5331671.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DABFOY010000035 EMBL· GenBank· DDBJ | HAI8281342.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DABFUC010000001 EMBL· GenBank· DDBJ | HAI8955932.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DABGKZ010000002 EMBL· GenBank· DDBJ | HAJ5148809.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DABHXT010000031 EMBL· GenBank· DDBJ | HAJ5960100.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DABWER010000207 EMBL· GenBank· DDBJ | HAO0516170.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DADRWU010000030 EMBL· GenBank· DDBJ | HBA4247994.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DAERAN010000058 EMBL· GenBank· DDBJ | HBH4490230.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
VZEL01000024 EMBL· GenBank· DDBJ | KAB0122593.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JACGTG010000001 EMBL· GenBank· DDBJ | MBA6241652.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAOVKC010000006 EMBL· GenBank· DDBJ | MCV5621478.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
RNLZ01000007 EMBL· GenBank· DDBJ | MGE13169.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WSXK01000039 EMBL· GenBank· DDBJ | MWF96358.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WTMY01000193 EMBL· GenBank· DDBJ | MWL47241.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WUIY01000254 EMBL· GenBank· DDBJ | MXI77044.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WXKQ01000002 EMBL· GenBank· DDBJ | NAG18378.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JACCPE010000028 EMBL· GenBank· DDBJ | NZD15530.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MPGR01000001 EMBL· GenBank· DDBJ | OKB75111.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
NNAF01000033 EMBL· GenBank· DDBJ | OZO78113.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP057293 EMBL· GenBank· DDBJ | QMF67375.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP057906 EMBL· GenBank· DDBJ | QMO38969.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP063369 EMBL· GenBank· DDBJ | QOY31794.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP065611 EMBL· GenBank· DDBJ | QPR05708.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP070393 EMBL· GenBank· DDBJ | QRZ95501.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QOGZ01000035 EMBL· GenBank· DDBJ | RDA34553.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
RRVG01000003 EMBL· GenBank· DDBJ | RRL50198.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
SCIU01000034 EMBL· GenBank· DDBJ | RXB27573.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UARS01000009 EMBL· GenBank· DDBJ | SPW54775.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UFXW01000004 EMBL· GenBank· DDBJ | STC85269.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UFZA01000002 EMBL· GenBank· DDBJ | STE71544.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UFYN01000009 EMBL· GenBank· DDBJ | STE77685.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UFZQ01000001 EMBL· GenBank· DDBJ | STE85461.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UGCO01000001 EMBL· GenBank· DDBJ | STI75309.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UGEA01000005 EMBL· GenBank· DDBJ | STK48850.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UINH01000002 EMBL· GenBank· DDBJ | SVF08682.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
VSBS01000682 EMBL· GenBank· DDBJ | TXT00347.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QYMS01000026 EMBL· GenBank· DDBJ | TXW77662.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CAADJZ010000001 EMBL· GenBank· DDBJ | VFT68795.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CABWJN010000003 EMBL· GenBank· DDBJ | VWN03278.1 EMBL· GenBank· DDBJ | Genomic DNA |