A0A024L2A9 · A0A024L2A9_ECOLX

  • Protein
    Protein/nucleic acid deglycase HchA
  • Gene
    hchA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Plays an important role in protecting cells from carbonyl stress.

Catalytic activity

  • H2O + N2-(1-hydroxy-2-oxoethyl)-GDP = GDP + glycolate + H+
  • H2O + N2-(1-hydroxy-2-oxoethyl)-GMP = glycolate + GMP + H+
  • H2O + N2-(1-hydroxy-2-oxoethyl)-GTP = glycolate + GTP + H+
  • H2O + N2-(1-hydroxy-2-oxoethyl)-dGTP = dGTP + glycolate + H+
  • H2O + N2-(1-hydroxy-2-oxopropyl)-GDP = GDP + H+ + lactate
  • H2O + N2-(1-hydroxy-2-oxopropyl)-GMP = GMP + H+ + lactate
  • H2O + N2-(1-hydroxy-2-oxopropyl)-GTP = GTP + H+ + lactate
  • H2O + N2-(1-hydroxy-2-oxopropyl)-dGTP = dGTP + H+ + lactate
  • H2O + N6-(1-hydroxy-2-oxoethyl)-L-lysyl-[protein] = glycolate + H+ + L-lysyl-[protein]
  • H2O + N6-(1-hydroxy-2-oxopropyl)-L-lysyl-[protein] = H+ + L-lysyl-[protein] + lactate
    EC:3.5.1.124 (UniProtKB | ENZYME | Rhea)
  • H2O + N(omega)-(1-hydroxy-2-oxoethyl)-L-arginyl-[protein] = glycolate + H+ + L-arginyl-[protein]
  • H2O + N(omega)-(1-hydroxy-2-oxopropyl)-L-arginyl-[protein] = H+ + L-arginyl-[protein] + lactate
    EC:3.5.1.124 (UniProtKB | ENZYME | Rhea)
  • H2O + S-(1-hydroxy-2-oxoethyl)-L-cysteinyl-[protein] = glycolate + H+ + L-cysteinyl-[protein]
  • H2O + S-(1-hydroxy-2-oxopropyl)-L-cysteinyl-[protein] = H+ + L-cysteinyl-[protein] + lactate
    EC:3.5.1.124 (UniProtKB | ENZYME | Rhea)
  • H2O + methylglyoxal = (R)-lactate + H+
    EC:4.2.1.130 (UniProtKB | ENZYME | Rhea)
  • an N2-(1-hydroxy-2-oxoethyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + glycolate + H+
  • an N2-(1-hydroxy-2-oxoethyl)-guanosine in RNA + H2O = a guanosine in RNA + glycolate + H+
  • an N2-(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + H+ + lactate
  • an N2-(1-hydroxy-2-oxopropyl)-guanosine in RNA + H2O = a guanosine in RNA + H+ + lactate

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site86Zn2+ (UniProtKB | ChEBI)
Binding site91Zn2+ (UniProtKB | ChEBI)
Binding site123Zn2+ (UniProtKB | ChEBI)
Active site185Nucleophile

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionlyase activity
Molecular Functionprotein deglycase activity
Molecular Functionthiolester hydrolase activity
Molecular Functionzinc ion binding
Biological ProcessDNA repair
Biological Processprotein repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein/nucleic acid deglycase HchA
  • EC number
  • Alternative names
    • Maillard deglycase

Gene names

    • Name
      hchA
    • ORF names
      ABT96_003721
      , ACW72_003908
      , BG944_002806
      , BGZ_01653
      , BMT50_21300
      , BvCmsKKP061_00566
      , BvCmsNSP007_03329
      , BVE17_005059
      , C3F40_00665
      , C719_002832
      , CG704_14640
      , CLG78_003595
      , D4M65_13585
      , D9D43_06110
      , DTL43_21780
      , DU321_04440
      , EPS97_16570
      , ExPECSC038_01920
      , F7F11_20115
      , FOI11_003080
      , FOI11_16965
      , FWK02_18105
      , GAI89_23735
      , GQE86_14965
      , GQM04_17335
      , GRW05_22880
      , GUC01_04925
      , H0O72_18450
      , HHH44_001696
      , HI055_003004
      , HIA71_15145
      , HJ488_004094
      , HJQ60_001626
      , HKA49_000011
      , HLZ50_02125
      , HMV95_17840
      , HVW43_01085
      , HVY77_10520
      , I6H02_04490
      , IG021_005099
      , J5U05_003154
      , JNP96_16515
      , KTF03_004586
      , NCTC10082_04431
      , NCTC10418_03071
      , NCTC10767_03558
      , NCTC10974_02300
      , NCTC11126_04427
      , NCTC11181_05650
      , NCTC8985_00529
      , NCTC9703_00277
      , OFN31_06760
      , SAMEA3472112_00448

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • 453
    • 1199
    • 1409
    • 376025
    • WW601
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    A0A024L2A9
  • Secondary accessions
    • A0A236LSQ8
    • A0A2A2XKN4

Proteomes

Subcellular Location

Keywords

Expression

Induction

By heat shock.

Interaction

Subunit

Homodimer.

Family & Domains

Sequence similarities

Belongs to the peptidase C56 family. HSP31-like subfamily.
Belongs to the peptidase C56 family. HchA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    283
  • Mass (Da)
    31,176
  • Last updated
    2014-07-09 v1
  • Checksum
    5CA8BE99F9A83B72
MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQEMLAAYAG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP026399
EMBL· GenBank· DDBJ
AUY00460.1
EMBL· GenBank· DDBJ
Genomic DNA
AASLUY010000028
EMBL· GenBank· DDBJ
EFE0301295.1
EMBL· GenBank· DDBJ
Genomic DNA
AASQZC010000089
EMBL· GenBank· DDBJ
EFF7861581.1
EMBL· GenBank· DDBJ
Genomic DNA
AASWKH010000034
EMBL· GenBank· DDBJ
EFH6097615.1
EMBL· GenBank· DDBJ
Genomic DNA
AASXRC010000013
EMBL· GenBank· DDBJ
EFI0213629.1
EMBL· GenBank· DDBJ
Genomic DNA
AATGUC010000026
EMBL· GenBank· DDBJ
EFL0834603.1
EMBL· GenBank· DDBJ
Genomic DNA
AATHUK010000024
EMBL· GenBank· DDBJ
EFL4330783.1
EMBL· GenBank· DDBJ
Genomic DNA
AATJOC010000010
EMBL· GenBank· DDBJ
EFM0253629.1
EMBL· GenBank· DDBJ
Genomic DNA
BFIH01000036
EMBL· GenBank· DDBJ
GCO24801.1
EMBL· GenBank· DDBJ
Genomic DNA
BFXY01000023
EMBL· GenBank· DDBJ
GDH29133.1
EMBL· GenBank· DDBJ
Genomic DNA
BGDN01000132
EMBL· GenBank· DDBJ
GDP81692.1
EMBL· GenBank· DDBJ
Genomic DNA
DABALL010000007
EMBL· GenBank· DDBJ
HAH1418325.1
EMBL· GenBank· DDBJ
Genomic DNA
DABCAF010000018
EMBL· GenBank· DDBJ
HAH6602868.1
EMBL· GenBank· DDBJ
Genomic DNA
DABDSA010000017
EMBL· GenBank· DDBJ
HAI2142651.1
EMBL· GenBank· DDBJ
Genomic DNA
DABERK010000007
EMBL· GenBank· DDBJ
HAI5331671.1
EMBL· GenBank· DDBJ
Genomic DNA
DABFOY010000035
EMBL· GenBank· DDBJ
HAI8281342.1
EMBL· GenBank· DDBJ
Genomic DNA
DABFUC010000001
EMBL· GenBank· DDBJ
HAI8955932.1
EMBL· GenBank· DDBJ
Genomic DNA
DABGKZ010000002
EMBL· GenBank· DDBJ
HAJ5148809.1
EMBL· GenBank· DDBJ
Genomic DNA
DABHXT010000031
EMBL· GenBank· DDBJ
HAJ5960100.1
EMBL· GenBank· DDBJ
Genomic DNA
DABWER010000207
EMBL· GenBank· DDBJ
HAO0516170.1
EMBL· GenBank· DDBJ
Genomic DNA
DADRWU010000030
EMBL· GenBank· DDBJ
HBA4247994.1
EMBL· GenBank· DDBJ
Genomic DNA
DAERAN010000058
EMBL· GenBank· DDBJ
HBH4490230.1
EMBL· GenBank· DDBJ
Genomic DNA
VZEL01000024
EMBL· GenBank· DDBJ
KAB0122593.1
EMBL· GenBank· DDBJ
Genomic DNA
JACGTG010000001
EMBL· GenBank· DDBJ
MBA6241652.1
EMBL· GenBank· DDBJ
Genomic DNA
JAOVKC010000006
EMBL· GenBank· DDBJ
MCV5621478.1
EMBL· GenBank· DDBJ
Genomic DNA
RNLZ01000007
EMBL· GenBank· DDBJ
MGE13169.1
EMBL· GenBank· DDBJ
Genomic DNA
WSXK01000039
EMBL· GenBank· DDBJ
MWF96358.1
EMBL· GenBank· DDBJ
Genomic DNA
WTMY01000193
EMBL· GenBank· DDBJ
MWL47241.1
EMBL· GenBank· DDBJ
Genomic DNA
WUIY01000254
EMBL· GenBank· DDBJ
MXI77044.1
EMBL· GenBank· DDBJ
Genomic DNA
WXKQ01000002
EMBL· GenBank· DDBJ
NAG18378.1
EMBL· GenBank· DDBJ
Genomic DNA
JACCPE010000028
EMBL· GenBank· DDBJ
NZD15530.1
EMBL· GenBank· DDBJ
Genomic DNA
MPGR01000001
EMBL· GenBank· DDBJ
OKB75111.1
EMBL· GenBank· DDBJ
Genomic DNA
NNAF01000033
EMBL· GenBank· DDBJ
OZO78113.1
EMBL· GenBank· DDBJ
Genomic DNA
CP057293
EMBL· GenBank· DDBJ
QMF67375.1
EMBL· GenBank· DDBJ
Genomic DNA
CP057906
EMBL· GenBank· DDBJ
QMO38969.1
EMBL· GenBank· DDBJ
Genomic DNA
CP063369
EMBL· GenBank· DDBJ
QOY31794.1
EMBL· GenBank· DDBJ
Genomic DNA
CP065611
EMBL· GenBank· DDBJ
QPR05708.1
EMBL· GenBank· DDBJ
Genomic DNA
CP070393
EMBL· GenBank· DDBJ
QRZ95501.1
EMBL· GenBank· DDBJ
Genomic DNA
QOGZ01000035
EMBL· GenBank· DDBJ
RDA34553.1
EMBL· GenBank· DDBJ
Genomic DNA
RRVG01000003
EMBL· GenBank· DDBJ
RRL50198.1
EMBL· GenBank· DDBJ
Genomic DNA
SCIU01000034
EMBL· GenBank· DDBJ
RXB27573.1
EMBL· GenBank· DDBJ
Genomic DNA
UARS01000009
EMBL· GenBank· DDBJ
SPW54775.1
EMBL· GenBank· DDBJ
Genomic DNA
UFXW01000004
EMBL· GenBank· DDBJ
STC85269.1
EMBL· GenBank· DDBJ
Genomic DNA
UFZA01000002
EMBL· GenBank· DDBJ
STE71544.1
EMBL· GenBank· DDBJ
Genomic DNA
UFYN01000009
EMBL· GenBank· DDBJ
STE77685.1
EMBL· GenBank· DDBJ
Genomic DNA
UFZQ01000001
EMBL· GenBank· DDBJ
STE85461.1
EMBL· GenBank· DDBJ
Genomic DNA
UGCO01000001
EMBL· GenBank· DDBJ
STI75309.1
EMBL· GenBank· DDBJ
Genomic DNA
UGEA01000005
EMBL· GenBank· DDBJ
STK48850.1
EMBL· GenBank· DDBJ
Genomic DNA
UINH01000002
EMBL· GenBank· DDBJ
SVF08682.1
EMBL· GenBank· DDBJ
Genomic DNA
VSBS01000682
EMBL· GenBank· DDBJ
TXT00347.1
EMBL· GenBank· DDBJ
Genomic DNA
QYMS01000026
EMBL· GenBank· DDBJ
TXW77662.1
EMBL· GenBank· DDBJ
Genomic DNA
CAADJZ010000001
EMBL· GenBank· DDBJ
VFT68795.1
EMBL· GenBank· DDBJ
Genomic DNA
CABWJN010000003
EMBL· GenBank· DDBJ
VWN03278.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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