A0A024L0P3 · A0A024L0P3_ECOLX
- ProteinBifunctional polymyxin resistance protein ArnA
- GenearnA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids660 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Bifunctional enzyme that catalyzes the oxidative decarboxylation of UDP-glucuronic acid (UDP-GlcUA) to UDP-4-keto-arabinose (UDP-Ara4O) and the addition of a formyl group to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N) to form UDP-L-4-formamido-arabinose (UDP-L-Ara4FN). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides.
Catalytic activity
- UDP-4-amino-4-deoxy-beta-L-arabinose + (6R)-10-formyltetrahydrofolate = UDP-4-deoxy-4-formamido-beta-L-arabinose + (6S)-5,6,7,8-tetrahydrofolate + H+
Pathway
Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 1/3.
Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 3/3.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 86-88 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | |||
Site | 102 | Transition state stabilizer | |||
Active site | 104 | Proton donor; for formyltransferase activity | |||
Binding site | 114 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | |||
Binding site | 136-140 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | |||
Site | 140 | Raises pKa of active site His | |||
Binding site | 347 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 368-369 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 393 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Binding site | 398 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Binding site | 432-433 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Active site | 434 | Proton acceptor; for decarboxylase activity | |||
Binding site | 460 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Binding site | 492 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Binding site | 526-535 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Binding site | 613 | UDP-alpha-D-glucuronate (UniProtKB | ChEBI) | |||
Active site | 619 | Proton donor; for decarboxylase activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carboxy-lyase activity | |
Molecular Function | UDP-4-amino-4-deoxy-L-arabinose formyltransferase activity | |
Molecular Function | UDP-glucuronic acid dehydrogenase activity | |
Biological Process | lipid A biosynthetic process | |
Biological Process | lipopolysaccharide biosynthetic process | |
Biological Process | response to antibiotic |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBifunctional polymyxin resistance protein ArnA
Including 2 domains:
- Recommended nameUDP-4-amino-4-deoxy-L-arabinose formyltransferase
- EC number
- Alternative names
- Recommended nameUDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acid decarboxylating
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionA0A024L0P3
- Secondary accessions
Proteomes
Interaction
Subunit
Homohexamer, formed by a dimer of trimers.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-304 | Formyltransferase ArnAFT | |||
Domain | 49-176 | Formyl transferase N-terminal | |||
Domain | 202-294 | Formyl transferase C-terminal | |||
Region | 314-660 | Dehydrogenase ArnADH | |||
Domain | 318-566 | NAD-dependent epimerase/dehydratase | |||
Sequence similarities
In the C-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.
In the N-terminal section; belongs to the Fmt family. UDP-L-Ara4N formyltransferase subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length660
- Mass (Da)74,289
- Last updated2014-07-09 v1
- ChecksumA430928AB4041FA3
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP026399 EMBL· GenBank· DDBJ | AUY04899.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AASFZR010000004 EMBL· GenBank· DDBJ | EFB4531187.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AASHPR010000042 EMBL· GenBank· DDBJ | EFC3526589.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AASEBA010000001 EMBL· GenBank· DDBJ | EFC9747731.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AASURL010000088 EMBL· GenBank· DDBJ | EFH0367618.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AASWOY010000016 EMBL· GenBank· DDBJ | EFH6648876.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAVTXU010000040 EMBL· GenBank· DDBJ | EGE1988333.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
ABLFQU030000001 EMBL· GenBank· DDBJ | EMM0023659.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DABHXT010000007 EMBL· GenBank· DDBJ | HAJ5958061.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DADUEU010000001 EMBL· GenBank· DDBJ | HBB1571211.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LDYI01000178 EMBL· GenBank· DDBJ | KPO03829.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JACZOI010000105 EMBL· GenBank· DDBJ | MBE0980095.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAWIHY010000005 EMBL· GenBank· DDBJ | MDV1986742.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WTML01000184 EMBL· GenBank· DDBJ | MWL00219.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WTQQ01000030 EMBL· GenBank· DDBJ | MWR87583.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WTRC01000079 EMBL· GenBank· DDBJ | MWT20889.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WTRX01000015 EMBL· GenBank· DDBJ | MWU31595.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WUIY01000004 EMBL· GenBank· DDBJ | MXI73110.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAAGYI010000001 EMBL· GenBank· DDBJ | NEM84033.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JABUPU010000001 EMBL· GenBank· DDBJ | NYP83768.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JABUPJ010000003 EMBL· GenBank· DDBJ | NYQ37842.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MPAF01000011 EMBL· GenBank· DDBJ | OOK29486.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP070393 EMBL· GenBank· DDBJ | QRZ95856.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UGDD01000002 EMBL· GenBank· DDBJ | STJ54043.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UGEX01000001 EMBL· GenBank· DDBJ | STL89070.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UGGJ01000006 EMBL· GenBank· DDBJ | STO40567.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UWVU01000001 EMBL· GenBank· DDBJ | VCW51261.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CABWJB010000001 EMBL· GenBank· DDBJ | VWM82844.1 EMBL· GenBank· DDBJ | Genomic DNA |