A0A024L0P3 · A0A024L0P3_ECOLX

  • Protein
    Bifunctional polymyxin resistance protein ArnA
  • Gene
    arnA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Bifunctional enzyme that catalyzes the oxidative decarboxylation of UDP-glucuronic acid (UDP-GlcUA) to UDP-4-keto-arabinose (UDP-Ara4O) and the addition of a formyl group to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N) to form UDP-L-4-formamido-arabinose (UDP-L-Ara4FN). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides.

Catalytic activity

Pathway

Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 1/3.
Nucleotide-sugar biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis; UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate: step 3/3.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site86-88(6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI)
Site102Transition state stabilizer
Active site104Proton donor; for formyltransferase activity
Binding site114(6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI)
Binding site136-140(6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI)
Site140Raises pKa of active site His
Binding site347NAD+ (UniProtKB | ChEBI)
Binding site368-369NAD+ (UniProtKB | ChEBI)
Binding site393UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site398UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site432-433UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Active site434Proton acceptor; for decarboxylase activity
Binding site460UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site492UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site526-535UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Binding site613UDP-alpha-D-glucuronate (UniProtKB | ChEBI)
Active site619Proton donor; for decarboxylase activity

GO annotations

AspectTerm
Molecular Functioncarboxy-lyase activity
Molecular FunctionUDP-4-amino-4-deoxy-L-arabinose formyltransferase activity
Molecular FunctionUDP-glucuronic acid dehydrogenase activity
Biological Processlipid A biosynthetic process
Biological Processlipopolysaccharide biosynthetic process
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional polymyxin resistance protein ArnA

Including 2 domains:

  • Recommended name
    UDP-4-amino-4-deoxy-L-arabinose formyltransferase
  • EC number
  • Alternative names
    • ArnAFT
    • UDP-L-Ara4N formyltransferase
  • Recommended name
    UDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acid decarboxylating
  • EC number
  • Alternative names
    • ArnADH
    • UDP-GlcUA decarboxylase
    • UDP-glucuronic acid dehydrogenase

Gene names

    • Name
      arnA
    • ORF names
      ACU57_26360
      , BANRA_00821
      , BGM66_004122
      , BGZ_03705
      , BMT91_07635
      , C0P57_000365
      , C3F40_26005
      , CTR35_003795
      , DL968_11915
      , E6D34_00225
      , G3V95_00735
      , G4A38_04290
      , G4A47_00700
      , GNW61_08980
      , GP944_12480
      , GP965_08075
      , GP979_04525
      , GQM21_24185
      , GRW05_02055
      , HMV95_07195
      , IH772_22955
      , J0541_000046
      , JNP96_18560
      , NCTC10429_02575
      , NCTC9045_01913
      , NCTC9706_04879
      , P6223_000148
      , RZR61_04655

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • 412057
    • AW1.3
    • 2.3-R4
    • Escherichia coli
    • EuSCAPE_DE065
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    A0A024L0P3
  • Secondary accessions
    • A0A236Q0Q9

Proteomes

Interaction

Subunit

Homohexamer, formed by a dimer of trimers.

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-304Formyltransferase ArnAFT
Domain49-176Formyl transferase N-terminal
Domain202-294Formyl transferase C-terminal
Region314-660Dehydrogenase ArnADH
Domain318-566NAD-dependent epimerase/dehydratase

Sequence similarities

In the C-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.
In the N-terminal section; belongs to the Fmt family. UDP-L-Ara4N formyltransferase subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    660
  • Mass (Da)
    74,289
  • Last updated
    2014-07-09 v1
  • Checksum
    A430928AB4041FA3
MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGSVARLAAERGIPVYAPDNVNHPLWVERIAQLSPDVIFSFYYRHLIYDEILQLAPAGAFNLHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAITLHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLHNMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEIVTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTERLLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSNLIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGSSRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIGNPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIRNAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP026399
EMBL· GenBank· DDBJ
AUY04899.1
EMBL· GenBank· DDBJ
Genomic DNA
AASFZR010000004
EMBL· GenBank· DDBJ
EFB4531187.1
EMBL· GenBank· DDBJ
Genomic DNA
AASHPR010000042
EMBL· GenBank· DDBJ
EFC3526589.1
EMBL· GenBank· DDBJ
Genomic DNA
AASEBA010000001
EMBL· GenBank· DDBJ
EFC9747731.1
EMBL· GenBank· DDBJ
Genomic DNA
AASURL010000088
EMBL· GenBank· DDBJ
EFH0367618.1
EMBL· GenBank· DDBJ
Genomic DNA
AASWOY010000016
EMBL· GenBank· DDBJ
EFH6648876.1
EMBL· GenBank· DDBJ
Genomic DNA
AAVTXU010000040
EMBL· GenBank· DDBJ
EGE1988333.1
EMBL· GenBank· DDBJ
Genomic DNA
ABLFQU030000001
EMBL· GenBank· DDBJ
EMM0023659.1
EMBL· GenBank· DDBJ
Genomic DNA
DABHXT010000007
EMBL· GenBank· DDBJ
HAJ5958061.1
EMBL· GenBank· DDBJ
Genomic DNA
DADUEU010000001
EMBL· GenBank· DDBJ
HBB1571211.1
EMBL· GenBank· DDBJ
Genomic DNA
LDYI01000178
EMBL· GenBank· DDBJ
KPO03829.1
EMBL· GenBank· DDBJ
Genomic DNA
JACZOI010000105
EMBL· GenBank· DDBJ
MBE0980095.1
EMBL· GenBank· DDBJ
Genomic DNA
JAWIHY010000005
EMBL· GenBank· DDBJ
MDV1986742.1
EMBL· GenBank· DDBJ
Genomic DNA
WTML01000184
EMBL· GenBank· DDBJ
MWL00219.1
EMBL· GenBank· DDBJ
Genomic DNA
WTQQ01000030
EMBL· GenBank· DDBJ
MWR87583.1
EMBL· GenBank· DDBJ
Genomic DNA
WTRC01000079
EMBL· GenBank· DDBJ
MWT20889.1
EMBL· GenBank· DDBJ
Genomic DNA
WTRX01000015
EMBL· GenBank· DDBJ
MWU31595.1
EMBL· GenBank· DDBJ
Genomic DNA
WUIY01000004
EMBL· GenBank· DDBJ
MXI73110.1
EMBL· GenBank· DDBJ
Genomic DNA
JAAGYI010000001
EMBL· GenBank· DDBJ
NEM84033.1
EMBL· GenBank· DDBJ
Genomic DNA
JABUPU010000001
EMBL· GenBank· DDBJ
NYP83768.1
EMBL· GenBank· DDBJ
Genomic DNA
JABUPJ010000003
EMBL· GenBank· DDBJ
NYQ37842.1
EMBL· GenBank· DDBJ
Genomic DNA
MPAF01000011
EMBL· GenBank· DDBJ
OOK29486.1
EMBL· GenBank· DDBJ
Genomic DNA
CP070393
EMBL· GenBank· DDBJ
QRZ95856.1
EMBL· GenBank· DDBJ
Genomic DNA
UGDD01000002
EMBL· GenBank· DDBJ
STJ54043.1
EMBL· GenBank· DDBJ
Genomic DNA
UGEX01000001
EMBL· GenBank· DDBJ
STL89070.1
EMBL· GenBank· DDBJ
Genomic DNA
UGGJ01000006
EMBL· GenBank· DDBJ
STO40567.1
EMBL· GenBank· DDBJ
Genomic DNA
UWVU01000001
EMBL· GenBank· DDBJ
VCW51261.1
EMBL· GenBank· DDBJ
Genomic DNA
CABWJB010000001
EMBL· GenBank· DDBJ
VWM82844.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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