A0A023X6S2 · A0A023X6S2_9ACTN

Function

function

Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.

Miscellaneous

Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site28Mg2+ 4 (UniProtKB | ChEBI)
Binding site28Mg2+ 3 (UniProtKB | ChEBI)
Binding site44Mg2+ 1 (UniProtKB | ChEBI)
Binding site44Mg2+ 2 (UniProtKB | ChEBI)
Binding site51substrate
Binding site72Mg2+ 2 (UniProtKB | ChEBI)
Binding site72Mg2+ 3 (UniProtKB | ChEBI)
Binding site72Mg2+ 4 (UniProtKB | ChEBI)
Binding site116-117ATP (UniProtKB | ChEBI)
Binding site117Mg2+ 1 (UniProtKB | ChEBI)
Binding site141ATP (UniProtKB | ChEBI)
Binding site203Mg2+ 3 (UniProtKB | ChEBI)
Binding site205ATP (UniProtKB | ChEBI)
Binding site206Mg2+ 5 (UniProtKB | ChEBI)
Binding site256substrate
Binding site307substrate

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionthiamine-phosphate kinase activity
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thiamine-monophosphate kinase
  • EC number
  • Short names
    TMP kinase
    ; Thiamine-phosphate kinase

Gene names

    • Name
      thiL
    • ORF names
      RradSPS_2427

Organism names

  • Taxonomic identifier
  • Strain
    • RSPS-4
  • Taxonomic lineage
    Bacteria > Actinomycetota > Rubrobacteria > Rubrobacterales > Rubrobacteraceae > Rubrobacter

Accessions

  • Primary accession
    A0A023X6S2

Proteomes

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain26-133PurM-like N-terminal
Domain145-293PurM-like C-terminal

Sequence similarities

Belongs to the thiamine-monophosphate kinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    312
  • Mass (Da)
    32,648
  • Last updated
    2014-07-09 v1
  • Checksum
    66006C89C8F6936E
MNEFEAIDRMAALLPERPDDVLVSIGDDCAVFRIGEERWVAAADMLVSGRHFLPAADPEDVGYKAVAVNVSDAAAMGARPRFVLTSAAAPNSETALACFRGVAAACREFGVYPLGGDTTGSDALVVDVAILGVLDGEPVLRSGARAGDLIAVTGELGASEAGLLALTEGIGGFERLKERHRRPEPRLEAGLVAARSGAGAMIDLSDGLASDLRHICRASGVGATVHLERLPLADDTRKLASLLRREPEQLAATGGEDFELLVTGSEAALKRIERASGIPVTPIGEVTVGDDVAFLLNGERIEGLSGWDHFET

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP007514
EMBL· GenBank· DDBJ
AHY47710.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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