A0A023X657 · A0A023X657_RUBRA

Function

function

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (L-Gln route): step 1/1.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site60Proton acceptor
Active site60Proton acceptor; for glutaminase activity
Active site127For glutaminase activity
Binding site133L-glutamine (UniProtKB | ChEBI)
Active site163Nucleophile; for glutaminase activity
Binding site190L-glutamine (UniProtKB | ChEBI)
Binding site196L-glutamine (UniProtKB | ChEBI)
Binding site338-345ATP (UniProtKB | ChEBI)
Binding site421deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site445ATP (UniProtKB | ChEBI)
Binding site450deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site562deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionglutaminase activity
Molecular FunctionNAD+ synthase (glutamine-hydrolyzing) activity
Molecular FunctionNAD+ synthase activity
Molecular Functionnitrilase activity
Biological ProcessNAD biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glutamine-dependent NAD(+) synthetase
  • EC number
  • Alternative names
    • NAD(+) synthase [glutamine-hydrolyzing]

Gene names

    • Name
      nadE
    • ORF names
      RradSPS_2408

Organism names

Accessions

  • Primary accession
    A0A023X657

Proteomes

Subcellular Location

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain20-260CN hydrolase

Sequence similarities

Belongs to the NAD synthetase family.
In the C-terminal section; belongs to the NAD synthetase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    600
  • Mass (Da)
    65,935
  • Last updated
    2014-07-09 v1
  • Checksum
    7291F9822B054BF2
MKRPPLARRGHRRRGIIWRMRVALAQVNTTVGDIWGNVEKLSEALGRAVESGADLVAFPELALTGYPPEDLLLRPGFIGDNLKALEEFRACVPEDVVAAVGFVDLGLDLFNACAVISGGEVLDRYHKRYLPNYGVFDENRYFREGTGSSLLDLDGTLVGVNVCEDIWYPGGPGREQALGGASVLVNVSASPYHRLKGATRERMLSVRASDYGCYVVMCNLVGGQDELVFDGHSVVFDPEGELVARAKQFEEDLLLVDIFPEEALMRRLHDSRPRKEDRTGEAPCVVSVPVSVGKVSPVESEEPRREPVLPEEGEVLAALELGLRDYFGKNGFTKAVLGLSGGVDSSLVAAVAARALGPENVVGVLMPSRYTSDLSNTDAYALAEALGIETKVVPIGPAFDAYREMLADLFAGTEEDITEENLQSRVRGNIVMGLSNKFGWIVLSTGNKSEMSVGYSTLYGDMAGGFALIRDVPKTLVYRICRYINSSEGREVIPESVLTKEPSAELREDQRDSDSLPPYDVLDPILEAYIEEDKGIGQIVALGFDEADVRRVVGLVDRAEYKRRQAPVGIKVTTRSFGRDRRMPITNRYTERERPRLKTP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP007514
EMBL· GenBank· DDBJ
AHY47691.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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