A0A023PHE9 · A0A023PHE9_9MONO
- ProteinRNA-directed RNA polymerase L
- GeneL
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids2233 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.
RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.
Catalytic activity
- GTP + H2O = GDP + H+ + phosphate
- a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-homocysteine
- a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + H+ + S-adenosyl-L-homocysteine
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | host cell cytoplasm | |
Cellular Component | virion component | |
Molecular Function | ATP binding | |
Molecular Function | hydrolase activity | |
Molecular Function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity | |
Molecular Function | RNA-dependent RNA polymerase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameRNA-directed RNA polymerase L
- Short namesProtein L
- Alternative names
Including 5 domains:
- Recommended nameRNA-directed RNA polymerase
- EC number
- Recommended nameGTP phosphohydrolase
- EC number
- Recommended nameGDP polyribonucleotidyltransferase
- EC number
- Alternative names
- Recommended namemRNA (nucleoside-2'-O-)-methyltransferase
- EC number
- Short namesN1-2'-O-MTase
- Recommended namemRNA (guanine-N(7)-)-methyltransferase
- Short namesG-N7-MTase
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Negarnaviricota > Haploviricotina > Monjiviricetes > Mononegavirales > Paramyxoviridae > Orthoparamyxovirinae > Respirovirus > Respirovirus pneumoniae
Accessions
- Primary accessionA0A023PHE9
Proteomes
Subcellular Location
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 656-840 | RdRp catalytic | ||||
Sequence: VSCFLTTDLKKYCLNWRYESTALFGETCNQIFGLNKLFNWLHPRLEGSTIYVGDPYCPPSDKEHISLEDHPDSGFYVHNPRGGIEGFCQKLWTLISISAIHLAAVRIGVRVTAMVQGDNQAIAVTTRVPNNYDYRVKKEIVYKDVVRFFDSLREVMDDLGHELKLNETIISSKMFIYSKRIYYDG | ||||||
Domain | 1775-1982 | Mononegavirus-type SAM-dependent 2'-O-MTase | ||||
Sequence: RLFGINSTSCLKALELSQILMKEVNKDKDRLFLGEGAGAMLACYDATLGPAVNYYNSGLNITDVIGQRELKIFPSEVSLVGKKLGNVTQILNRVKVLFNGNPNSTWIGNMECESLIWSELNDKSIGLVHCDMEGAIGKSEETVLHEHYSVIRITYLIGDDDVVLVSKIIPTITPNWSRILYLYKLYWKDVSVISLKTSNPASTELYLI |
Sequence similarities
Belongs to the paramyxovirus L protein family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,233
- Mass (Da)255,957
- Last updated2014-07-09 v1
- ChecksumCC7FC21A44D38FA4
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KJ672532 EMBL· GenBank· DDBJ | AHX22095.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
KJ672616 EMBL· GenBank· DDBJ | AHX22527.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
KY674925 EMBL· GenBank· DDBJ | ARB07473.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
KY674939 EMBL· GenBank· DDBJ | ARB07585.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
KY973593 EMBL· GenBank· DDBJ | ARQ32825.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
KY973588 EMBL· GenBank· DDBJ | ARQ32865.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
KY973587 EMBL· GenBank· DDBJ | ARQ32873.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
KY986642 EMBL· GenBank· DDBJ | ARQ33209.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MF034889 EMBL· GenBank· DDBJ | ARX74152.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MF554716 EMBL· GenBank· DDBJ | ASY03312.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MF554717 EMBL· GenBank· DDBJ | ASY03320.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MF973170 EMBL· GenBank· DDBJ | ATY71429.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MF973185 EMBL· GenBank· DDBJ | ATY71549.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MF973189 EMBL· GenBank· DDBJ | ATY71581.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MT118713 EMBL· GenBank· DDBJ | QQY99552.1 EMBL· GenBank· DDBJ | Viral cRNA |