A0A023PHE9 · A0A023PHE9_9MONO

  • Protein
    RNA-directed RNA polymerase L
  • Gene
    L
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.
RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.

Catalytic activity

  • GTP + H2O = GDP + H+ + phosphate
  • a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-homocysteine
  • a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + 2 S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + H+ + 2 S-adenosyl-L-homocysteine
    EC:2.1.1.375 (UniProtKB | ENZYME | Rhea)
  • a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + H+ + S-adenosyl-L-homocysteine
  • a 5'-end triphospho-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + GDP + H+ = a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + diphosphate
    EC:2.7.7.88 (UniProtKB | ENZYME | Rhea)
  • a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
    EC:2.7.7.48 (UniProtKB | ENZYME | Rhea)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell cytoplasm
Cellular Componentvirion component
Molecular FunctionATP binding
Molecular Functionhydrolase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular FunctionRNA-dependent RNA polymerase activity

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    RNA-directed RNA polymerase L
  • Short names
    Protein L
  • Alternative names
    • Large structural protein
    • Replicase
    • Transcriptase

Including 5 domains:

  • Recommended name
    RNA-directed RNA polymerase
  • EC number
  • Recommended name
    GTP phosphohydrolase
  • EC number
  • Recommended name
    GDP polyribonucleotidyltransferase
  • EC number
  • Alternative names
    • PRNTase
  • Recommended name
    mRNA (nucleoside-2'-O-)-methyltransferase
  • EC number
  • Short names
    N1-2'-O-MTase
  • Recommended name
    mRNA (guanine-N(7)-)-methyltransferase
  • Short names
    G-N7-MTase

Gene names

    • Name
      L
    • ORF names
      AZ69_44284gpL
      , AZ69_44290gpL

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • HPIV3/Homo sapiens/PER/FLU8925/2007
    • HPIV3/Homo sapiens/PER/FLU9187/2007
    • HPIV3/USA/UAMS_034/2016
    • HPIV3/Seattle/USA/10G10/2010
    • HPIV3/Seattle/USA/10I8/2010
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Negarnaviricota > Haploviricotina > Monjiviricetes > Mononegavirales > Paramyxoviridae > Orthoparamyxovirinae > Respirovirus > Respirovirus pneumoniae

Accessions

  • Primary accession
    A0A023PHE9

Proteomes

Subcellular Location

Keywords

Structure

3D structure databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain656-840RdRp catalytic
Domain1775-1982Mononegavirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the paramyxovirus L protein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,233
  • Mass (Da)
    255,957
  • Last updated
    2014-07-09 v1
  • Checksum
    CC7FC21A44D38FA4
MDTESNNGTVSDILYPECHLNSPIVKGKIAQLHTIMSLPQPYDMDDDSILVITRQKIKLNKLDKRQRSIRRLKLILIEKVSDLGKYTFIRYPEMSKEMFKLHIPGINSKVTELLLKADRTYSQMTDGLRDLWINVLSKLASKNDGSNYDLNEEINNISKVHTTYKSDKWYNPFKTWFTIKYDMRRLQKARNEVTFNMGKDYNLLEDQKNLLLIHPELVLILDKQNYNGYLITPELVLMYCDVIEGRWNISACAKLDPKLQSMYQKGNNLWEVIDKLFPIMGEKTFDVISLLEPLALSLIQTHDPVKQLRGAFLNHVLSEMELIFESRESIKEFLSVDYIDKILDIFDKSTIDEIAEIFSFFRTFGHPPLEASIAAEKVRKYMYIEKQLKFDTINKCHAIFCTIIINGYRERHGGQWPPVTLPDHAHEFIINAYGSNSAISYENAVDYYQSFIGIKFNKFIEPQLDEDLTIYMKDKALSPKKSNWDTVYPASNLLYRTNASNESRRLVEVFIADSKFDPHQILDYVESGDWLDDPEFNISYSLKEKEIKQEGRLFAKMTYKMRATQVLSETLLANNIGKFFQENGMVKGEIELLKRLTTISISGVPRYNEVYNNSKSHTDDLKTYNKISNLNLSSNQKSKKFEFKSTDIYNDGYETVSCFLTTDLKKYCLNWRYESTALFGETCNQIFGLNKLFNWLHPRLEGSTIYVGDPYCPPSDKEHISLEDHPDSGFYVHNPRGGIEGFCQKLWTLISISAIHLAAVRIGVRVTAMVQGDNQAIAVTTRVPNNYDYRVKKEIVYKDVVRFFDSLREVMDDLGHELKLNETIISSKMFIYSKRIYYDGRILPQALKALSRCVFWSETVIDETRSASSNLATSFAKAIENGYSPVLGYACSIFKNIQQLYIALGMNINPTITQNIKDQYFRNPNWMQYASLIPASVGGFNYMAMSRCFVRNIGDPSVAALADIKRFIKANLLDRSVLYRIMNQEPGESSFLDWASDPYSCNLPQSQNITTMIKNITARNVLQDSPNPLLSGLFTNTMIEEDEELAEFLMDRKVILPRVAHDILDNSLTGIRNAIAGMLDTTKSLIRVGINRGGLTYSLLRKISNYDLAQYETLSRTLRLIVSDKIRYEDMCSVDLAIALRQKMWIHLSGGRMISGLETPDPLELLSGVVITGSEHCKICYSSDGTNPYTWMYLPGNIKIGSAETGISSLRVPYFGSVTDERSEAQLGYIKNLSKPAKAAIRIAMIYTWAFGNDEISWMEASQIAQTRANFTLDNLKILTPVATSTNLSHRLKDTATQMKFSSTSLIRVSRFITMSNDNMSIKEANETKDTNLIYQQIMLTGLSVFEYLFRLKETTGHNPIVMHLHIEDECCIKESFNDEHINPESTLELIRYPESNEFIYDKDPLKDVDLSRLMVIKDHSYTIDMNYWDDTDIIHAISICTAITIADTMSQLDRDNLKEIIVIANDDDINSLITEFLTLDILVFLKTFGGLLVNQFAYTLYSLKIEGRDLIWDYIMRTLRDTSHSILKVLSNALSHPKVFKRFWDCGVLNPIYGPNTASQDQIKLALSICEYSLDLFMREWLNGVSLEIYICDSDMEVANDRKQAFISRHLSFVCCLAEIASFGPNLLNLTYLERLDLLKQYLELNIKEDPTLKYVQISGLLIKSFPSTVTYVRKTAIKYLRIRGISPPEVIDDWDPIEDENMLDNIVKSINDNCNKDNKGNKINNFWGLALKNYQVLKIRSITSDSDNNDGLDVSTGGLTLPQGGNYLSHQLRLFGINSTSCLKALELSQILMKEVNKDKDRLFLGEGAGAMLACYDATLGPAVNYYNSGLNITDVIGQRELKIFPSEVSLVGKKLGNVTQILNRVKVLFNGNPNSTWIGNMECESLIWSELNDKSIGLVHCDMEGAIGKSEETVLHEHYSVIRITYLIGDDDVVLVSKIIPTITPNWSRILYLYKLYWKDVSVISLKTSNPASTELYLISKDAYCTIMEPSEVVLSKLKRLSLLEENNLLKWIILSKKRNNEWLHHEIKEGERDYGVMRPYHMALQIFGFQINLNHLAKEFLSTPDLTNINNIIQSFQRTIKDVLFEWINITHDDKRHKLGGRYNIFPLKNKGKLRLLSRRLVLSWISLSLSTRLLTGRFPDEKFEHRAQTGYVSLADTDLESLKLLSKNIIKNYKECIGSISYWFLTKEIKILMKLIGGAKLLGIPRQYKEPEEQLLENYNQHDEFDID

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KJ672532
EMBL· GenBank· DDBJ
AHX22095.1
EMBL· GenBank· DDBJ
Viral cRNA
KJ672616
EMBL· GenBank· DDBJ
AHX22527.1
EMBL· GenBank· DDBJ
Viral cRNA
KY674925
EMBL· GenBank· DDBJ
ARB07473.1
EMBL· GenBank· DDBJ
Viral cRNA
KY674939
EMBL· GenBank· DDBJ
ARB07585.1
EMBL· GenBank· DDBJ
Viral cRNA
KY973593
EMBL· GenBank· DDBJ
ARQ32825.1
EMBL· GenBank· DDBJ
Viral cRNA
KY973588
EMBL· GenBank· DDBJ
ARQ32865.1
EMBL· GenBank· DDBJ
Viral cRNA
KY973587
EMBL· GenBank· DDBJ
ARQ32873.1
EMBL· GenBank· DDBJ
Viral cRNA
KY986642
EMBL· GenBank· DDBJ
ARQ33209.1
EMBL· GenBank· DDBJ
Viral cRNA
MF034889
EMBL· GenBank· DDBJ
ARX74152.1
EMBL· GenBank· DDBJ
Viral cRNA
MF554716
EMBL· GenBank· DDBJ
ASY03312.1
EMBL· GenBank· DDBJ
Viral cRNA
MF554717
EMBL· GenBank· DDBJ
ASY03320.1
EMBL· GenBank· DDBJ
Viral cRNA
MF973170
EMBL· GenBank· DDBJ
ATY71429.1
EMBL· GenBank· DDBJ
Viral cRNA
MF973185
EMBL· GenBank· DDBJ
ATY71549.1
EMBL· GenBank· DDBJ
Viral cRNA
MF973189
EMBL· GenBank· DDBJ
ATY71581.1
EMBL· GenBank· DDBJ
Viral cRNA
MT118713
EMBL· GenBank· DDBJ
QQY99552.1
EMBL· GenBank· DDBJ
Viral cRNA

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