A0A022MV86 · A0A022MV86_9ACTN
- Protein3'-phosphate/5'-hydroxy nucleic acid ligase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids397 (go to sequence)
- Protein existencePredicted
- Annotation score2/5
Function
Catalytic activity
- a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate
Cofactor
Note: Binds 2 manganese ions per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 74 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 162-166 | GMP (UniProtKB | ChEBI) | |||
Binding site | 163 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 180 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 270 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 270-271 | GMP (UniProtKB | ChEBI) | |||
Binding site | 302-305 | GMP (UniProtKB | ChEBI) | |||
Binding site | 309 | GMP (UniProtKB | ChEBI) | |||
Active site | 326 | GMP-histidine intermediate | |||
Binding site | 326-329 | GMP (UniProtKB | ChEBI) | |||
Binding site | 396 | GMP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA ligase activity | |
Molecular Function | GTP binding | |
Molecular Function | manganese ion binding | |
Molecular Function | RNA ligase (GTP) activity | |
Biological Process | DNA repair | |
Biological Process | RNA processing | |
Biological Process | RNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3'-phosphate/5'-hydroxy nucleic acid ligase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces
Accessions
- Primary accessionA0A022MV86
Proteomes
Structure
Sequence
- Sequence statusComplete
- Length397
- Mass (Da)43,319
- Last updated2014-06-11 v1
- Checksum6D0E3205A2439004
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JFJQ01000005 EMBL· GenBank· DDBJ | EYT84665.1 EMBL· GenBank· DDBJ | Genomic DNA |