A0A022KZM8 · A0A022KZM8_9ACTN

  • Protein
    Thymidylate synthase
  • Gene
    thyA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site24dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site54(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site129-130dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Active site149
Active site149Nucleophile
Binding site169-172dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site172(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site180dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site210-212dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site265(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thymidylate synthase
  • EC number
  • Short names
    TS
    ; TSase

Gene names

    • Name
      thyA
    • ORF names
      H483_0116045

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • UCD-THP
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Dietziaceae > Dietzia

Accessions

  • Primary accession
    A0A022KZM8

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain6-266Thymidylate synthase/dCMP hydroxymethylase

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    266
  • Mass (Da)
    30,302
  • Last updated
    2014-06-11 v1
  • Checksum
    FCE74854FA7323D1
MTVPTPYEDLLRLVLEQGTPKSDRTGTGTRSLFGHQLRYDLAAGFPLITTKRVHFKSVAYELLWFLRGDSNVGWLRENGVSIWDEWADERGELGPVYGVQWRSWPTPDGRHVDQIAASVDLLRSDPDSRRNIVSAWNVGELSDMALPPCHLLFQFYVADGKLSCQLYQRSADLFLGVPFNIASYSLLTHMVAQQVGLEVGEFIWTGGDCHIYDNHVEQVRTQLSREAFPYPELRVRRADSIFDYRFEDFDVVGYEHHPGIKAPVAV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AOSR01000062
EMBL· GenBank· DDBJ
EYT56971.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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