A0A022KZM8 · A0A022KZM8_9ACTN
- ProteinThymidylate synthase
- GenethyA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids266 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.
Catalytic activity
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP
Pathway
Pyrimidine metabolism; dTTP biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 24 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | ||||
Sequence: R | ||||||
Binding site | 54 | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 129-130 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners | ||||
Sequence: RR | ||||||
Active site | 149 | |||||
Sequence: C | ||||||
Active site | 149 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 169-172 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | ||||
Sequence: RSAD | ||||||
Binding site | 172 | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 180 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | ||||
Sequence: N | ||||||
Binding site | 210-212 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | ||||
Sequence: HIY | ||||||
Binding site | 265 | (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: A |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | thymidylate synthase activity | |
Biological Process | dTMP biosynthetic process | |
Biological Process | dTTP biosynthetic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThymidylate synthase
- EC number
- Short namesTS ; TSase
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Dietziaceae > Dietzia
Accessions
- Primary accessionA0A022KZM8
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 6-266 | Thymidylate synthase/dCMP hydroxymethylase | ||||
Sequence: PYEDLLRLVLEQGTPKSDRTGTGTRSLFGHQLRYDLAAGFPLITTKRVHFKSVAYELLWFLRGDSNVGWLRENGVSIWDEWADERGELGPVYGVQWRSWPTPDGRHVDQIAASVDLLRSDPDSRRNIVSAWNVGELSDMALPPCHLLFQFYVADGKLSCQLYQRSADLFLGVPFNIASYSLLTHMVAQQVGLEVGEFIWTGGDCHIYDNHVEQVRTQLSREAFPYPELRVRRADSIFDYRFEDFDVVGYEHHPGIKAPVAV |
Sequence similarities
Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length266
- Mass (Da)30,302
- Last updated2014-06-11 v1
- ChecksumFCE74854FA7323D1