A0A014MLG5 · A0A014MLG5_9BURK
- ProteinUDP-2,3-diacylglucosamine hydrolase
- GenelpxH
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids267 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Hydrolyzes the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate (lipid X) and UMP by catalyzing the attack of water at the alpha-P atom. Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.
Catalytic activity
- UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine + H2O = 2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl 1-phosphate + UMP + 2 H+
Cofactor
Note: Binds 2 Mn2+ ions per subunit in a binuclear metal center.
Pathway
Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 4/6.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 24 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 26 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 55 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 55 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 95 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 95-96 | substrate | |||
Binding site | 130 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 138 | substrate | |||
Binding site | 176 | substrate | |||
Binding site | 210 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 210 | substrate | |||
Binding site | 212 | Mn2+ 1 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | extrinsic component of plasma membrane | |
Molecular Function | manganese ion binding | |
Molecular Function | UDP-2,3-diacylglucosamine hydrolase activity | |
Biological Process | lipid A biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUDP-2,3-diacylglucosamine hydrolase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Comamonas
Accessions
- Primary accessionA0A014MLG5
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Peripheral membrane protein
Keywords
- Cellular component
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length267
- Mass (Da)29,790
- Last updated2014-06-11 v1
- Checksum95A51F53E97D8133
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JBOK01000022 EMBL· GenBank· DDBJ | EXU78969.1 EMBL· GenBank· DDBJ | Genomic DNA |