A0A011PKT1 · A0A011PKT1_9PROT
- ProteintRNA modification GTPase MnmE
- GenemnmE
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids383 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.
Cofactor
Note: Binds 1 potassium ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 163 | K+ (UniProtKB | ChEBI) | |||
Binding site | 163-168 | GTP (UniProtKB | ChEBI) | |||
Binding site | 167 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 182 | K+ (UniProtKB | ChEBI) | |||
Binding site | 182-188 | GTP (UniProtKB | ChEBI) | |||
Binding site | 184 | K+ (UniProtKB | ChEBI) | |||
Binding site | 187 | K+ (UniProtKB | ChEBI) | |||
Binding site | 188 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 207-210 | GTP (UniProtKB | ChEBI) | |||
Binding site | 291-293 | GTP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | metal ion binding | |
Biological Process | tRNA methylation | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA modification GTPase MnmE
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Candidatus Accumulibacter
Accessions
- Primary accessionA0A011PKT1
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, coiled coil.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 153-310 | TrmE-type G | |||
Coiled coil | 321-355 | ||||
Sequence similarities
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length383
- Mass (Da)41,412
- Last updated2014-06-11 v1
- MD5 ChecksumA613F487B3A7116F7B7A405EB2D9C95C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JEMX01000098 EMBL· GenBank· DDBJ | EXI77420.1 EMBL· GenBank· DDBJ | Genomic DNA |