A0A011NY62 · A0A011NY62_9PROT
- ProteintRNA modification GTPase MnmE
- GenemnmE
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids458 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.
Cofactor
Note: Binds 1 potassium ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 36 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
Binding site | 93 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
Binding site | 132 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
Binding site | 238 | K+ (UniProtKB | ChEBI) | |||
Binding site | 238-243 | GTP (UniProtKB | ChEBI) | |||
Binding site | 242 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 257 | K+ (UniProtKB | ChEBI) | |||
Binding site | 257-263 | GTP (UniProtKB | ChEBI) | |||
Binding site | 259 | K+ (UniProtKB | ChEBI) | |||
Binding site | 262 | K+ (UniProtKB | ChEBI) | |||
Binding site | 263 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 282-285 | GTP (UniProtKB | ChEBI) | |||
Binding site | 366-368 | GTP (UniProtKB | ChEBI) | |||
Binding site | 458 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | metal ion binding | |
Biological Process | tRNA methylation | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA modification GTPase MnmE
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Candidatus Accumulibacter
Accessions
- Primary accessionA0A011NY62
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, coiled coil.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 228-385 | TrmE-type G | |||
Coiled coil | 396-440 | ||||
Sequence similarities
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length458
- Mass (Da)49,619
- Last updated2014-06-11 v1
- MD5 Checksum489EEC3549BD59CBB844D9CB0A0030EE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JFAX01000001 EMBL· GenBank· DDBJ | EXI69545.1 EMBL· GenBank· DDBJ | Genomic DNA |