T2GJZ6 · T2GJZ6_9EURY
- ProteinGlutamyl-tRNA(Gln) amidotransferase subunit D
- GenegatD
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids435 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.
Catalytic activity
- L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H+
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 101 | |||||
Sequence: T | ||||||
Active site | 177 | |||||
Sequence: T | ||||||
Active site | 178 | |||||
Sequence: D | ||||||
Active site | 254 | |||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | asparaginase activity | |
Molecular Function | ATP binding | |
Molecular Function | glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity | |
Molecular Function | transferase activity | |
Biological Process | amino acid metabolic process | |
Biological Process | regulation of translational fidelity | |
Biological Process | translation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamyl-tRNA(Gln) amidotransferase subunit D
- EC number
- Short namesGlu-ADT subunit D
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanobacteria > Methanobacteriales > Methanobacteriaceae > Methanothermobacter
Accessions
- Primary accessionT2GJZ6
Proteomes
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 16-69 | GatD N-terminal | ||||
Sequence: IDVGDMVRVEKPDVTYEGMVLDRADDADDRHIVLKLENGYNIGVEISDAMIELL | ||||||
Domain | 93-284 | L-asparaginase N-terminal | ||||
Sequence: VSIISTGGTVASIIDYRTGAVHPAFTADDLLRANPELLDIANIRGRAVFNILSENMKPEYWVETARAVYGEIKEGADGVVVAHGTDTMHYTSAALSFMLMTPVPVVFTGAQRSSDRPSSDASLNIQCSVRAATSEIAEVTVCMHATMDDLSCHLHRGVKVRKMHTSRRDTFRSMNALPLAEVTPDGIKILEE | ||||||
Domain | 303-416 | Asparaginase/glutaminase C-terminal | ||||
Sequence: RVAFIKSYPGISPDIIKWHLDEGYRGIVIEGTGLGHCPDTLIPVIREAHDMGVPVAMTSQCLNGRVNMNVYSTGRRLLQAGVIPCDDMLPEVAYVKMCWVLGQTDDPEMACEMM |
Sequence similarities
Belongs to the asparaginase 1 family. GatD subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length435
- Mass (Da)48,078
- Last updated2013-11-13 v1
- ChecksumC9F51582CDF16BCE
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP011952 EMBL· GenBank· DDBJ | BAM69883.1 EMBL· GenBank· DDBJ | Genomic DNA |