Q9UJX6 · ANC2_HUMAN

  • Protein
    Anaphase-promoting complex subunit 2
  • Gene
    ANAPC2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Together with the RING-H2 protein ANAPC11, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:11739784, PubMed:18485873).
The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:11739784, PubMed:18485873).
The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132).
The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons (By similarity).
CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation (By similarity).

Pathway

Protein modification; protein ubiquitination.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentanaphase-promoting complex
Cellular Componentcytosol
Cellular Componentnucleoplasm
Molecular Functionubiquitin protein ligase binding
Biological Processanaphase-promoting complex-dependent catabolic process
Biological Processcell differentiation
Biological Processcell division
Biological Processmetaphase/anaphase transition of mitotic cell cycle
Biological Processnegative regulation of gene expression
Biological Processnervous system development
Biological Processpositive regulation of axon extension
Biological Processpositive regulation of dendrite morphogenesis
Biological Processpositive regulation of synapse maturation
Biological Processpositive regulation of synaptic plasticity
Biological Processprotein branched polyubiquitination
Biological Processprotein K11-linked ubiquitination
Biological Processprotein K48-linked ubiquitination
Biological Processregulation of meiotic cell cycle
Biological Processregulation of mitotic cell cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Anaphase-promoting complex subunit 2
  • Short names
    APC2
  • Alternative names
    • Cyclosome subunit 2

Gene names

    • Name
      ANAPC2
    • Synonyms
      APC2, KIAA1406

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    Q9UJX6
  • Secondary accessions
    • Q5VSG1
    • Q96DG5
    • Q96GG4
    • Q9P2E1

Proteomes

Organism-specific databases

Disease & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis350Impairs UBE2S-mediated polyubiquitination, decreasing substrate affinity, does not affect UBE2C-mediated multiubiquitination; when associated with K-353.
Mutagenesis353Impairs UBE2S-mediated polyubiquitination, decreasing substrate affinity, does not affect UBE2C-mediated multiubiquitination; when associated with K-350.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 814 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Organism-specific databases

Miscellaneous

Genetic variation databases

PTM/Processing

Features

Showing features for chain, modified residue, modified residue (large scale data).

Type
IDPosition(s)Source
Description
ChainPRO_00001198111-822UniProtAnaphase-promoting complex subunit 2
Modified residue218UniProtPhosphoserine
Modified residue (large scale data)218PRIDEPhosphoserine
Modified residue314UniProtPhosphoserine
Modified residue (large scale data)314PRIDEPhosphoserine
Modified residue470UniProtPhosphoserine
Modified residue (large scale data)470PRIDEPhosphoserine
Modified residue (large scale data)532PRIDEPhosphoserine
Modified residue534UniProtPhosphoserine
Modified residue (large scale data)534PRIDEPhosphoserine
Modified residue697UniProtPhosphoserine
Modified residue (large scale data)732PRIDEPhosphoserine
Modified residue (large scale data)736PRIDEPhosphoserine
Modified residue810UniProtPhosphotyrosine
Modified residue (large scale data)810PRIDEPhosphotyrosine
Modified residue (large scale data)811PRIDEPhosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Organism-specific databases

Interaction

Subunit

The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5 (PubMed:25043029, PubMed:26083744).
In the context of the APC/C complex, directly interacts with UBE2C and UBE2S (PubMed:27259151).
Interacts (via cullin domain) with ANAPC11 and with UBCH10 (PubMed:11739784).
Interacts with NEUROD2 (By similarity).
Interacts with FBXO43; the interaction is direct

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY Q9UJX6FBXO5 Q9UKT4-17EBI-396211, EBI-16059332
BINARY Q9UJX6MEOX1 P502213EBI-396211, EBI-2864512
BINARY Q9UJX6UBE2S Q167634EBI-396211, EBI-2339823
BINARY Q9UJX6WDR5 P619643EBI-396211, EBI-540834

Complex viewer

View interactors in UniProtKB
View CPX-1860 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, compositional bias.

Type
IDPosition(s)Description
Region450-495Disordered
Compositional bias468-481Acidic residues
Region502-700Cullin homology

Sequence similarities

Belongs to the cullin family.

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q9UJX6-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    822
  • Mass (Da)
    93,828
  • Last updated
    2000-05-01 v1
  • MD5 Checksum
    EAB586AFEE2BA87486C9DA5E76110FC0
MAAAVVVAEGDSDSRPGQELLVAWNTVSTGLVPPAALGLVSSRTSGAVPPKEEELRAAVEVLRGHGLHSVLEEWFVEVLQNDLQANISPEFWNAISQCENSADEPQCLLLLLDAFGLLESRLDPYLRSLELLEKWTRLGLLMGTGAQGLREEVHTMLRGVLFFSTPRTFQEMIQRLYGCFLRVYMQSKRKGEGGTDPELEGELDSRYARRRYYRLLQSPLCAGCSSDKQQCWCRQALEQFHQLSQVLHRLSLLERVSAEAVTTTLHQVTRERMEDRCRGEYERSFLREFHKWIERVVGWLGKVFLQDGPARPASPEAGNTLRRWRCHVQRFFYRIYASLRIEELFSIVRDFPDSRPAIEDLKYCLERTDQRQQLLVSLKAALETRLLHPGVNTCDIITLYISAIKALRVLDPSMVILEVACEPIRRYLRTREDTVRQIVAGLTGDSDGTGDLAVELSKTDPASLETGQDSEDDSGEPEDWVPDPVDADPGKSSSKRRSSDIISLLVSIYGSKDLFINEYRSLLADRLLHQFSFSPEREIRNVELLKLRFGEAPMHFCEVMLKDMADSRRINANIREEDEKRPAEEQPPFGVYAVILSSEFWPPFKDEKLEVPEDIRAALEAYCKKYEQLKAMRTLSWKHTLGLVTMDVELADRTLSVAVTPVQAVILLYFQDQASWTLEELSKAVKMPVALLRRRMSVWLQQGVLREEPPGTFSVIEEERPQDRDNMVLIDSDDESDSGMASQADQKEEELLLFWTYIQAMLTNLESLSLDRIYNMLRMFVVTGPALAEIDLQELQGYLQKKVRDQQLVYSAGVYRLPKNCS

Q9UJX6-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Sequence caution

The sequence AAH09487.2 differs from that shown. Reason: Erroneous translation Wrong choice of frame.

Features

Showing features for alternative sequence, compositional bias.

Type
IDPosition(s)Description
Alternative sequenceVSP_008463350-352in isoform 2
Compositional bias468-481Acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF191337
EMBL· GenBank· DDBJ
AAF05751.1
EMBL· GenBank· DDBJ
mRNA
AL929554
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC032503
EMBL· GenBank· DDBJ
AAH32503.1
EMBL· GenBank· DDBJ
mRNA
BC001579
EMBL· GenBank· DDBJ
AAH01579.1
EMBL· GenBank· DDBJ
mRNA
BC009487
EMBL· GenBank· DDBJ
AAH09487.2
EMBL· GenBank· DDBJ
mRNA Sequence problems.
AB037827
EMBL· GenBank· DDBJ
BAA92644.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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