Q9UJX6 · ANC2_HUMAN
- ProteinAnaphase-promoting complex subunit 2
- GeneANAPC2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids822 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Together with the RING-H2 protein ANAPC11, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:11739784, PubMed:18485873).
The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:11739784, PubMed:18485873).
The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132).
The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons (By similarity).
CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation (By similarity).
The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:11739784, PubMed:18485873).
The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132).
The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons (By similarity).
CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation (By similarity).
Pathway
Protein modification; protein ubiquitination.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | anaphase-promoting complex | |
Cellular Component | cytosol | |
Cellular Component | nucleoplasm | |
Molecular Function | ubiquitin protein ligase binding | |
Biological Process | anaphase-promoting complex-dependent catabolic process | |
Biological Process | cell differentiation | |
Biological Process | cell division | |
Biological Process | metaphase/anaphase transition of mitotic cell cycle | |
Biological Process | negative regulation of gene expression | |
Biological Process | nervous system development | |
Biological Process | positive regulation of axon extension | |
Biological Process | positive regulation of dendrite morphogenesis | |
Biological Process | positive regulation of synapse maturation | |
Biological Process | positive regulation of synaptic plasticity | |
Biological Process | protein branched polyubiquitination | |
Biological Process | protein K11-linked ubiquitination | |
Biological Process | protein K48-linked ubiquitination | |
Biological Process | regulation of meiotic cell cycle | |
Biological Process | regulation of mitotic cell cycle |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAnaphase-promoting complex subunit 2
- Short namesAPC2
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ9UJX6
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Disease & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 350 | Impairs UBE2S-mediated polyubiquitination, decreasing substrate affinity, does not affect UBE2C-mediated multiubiquitination; when associated with K-353. | |||
Mutagenesis | 353 | Impairs UBE2S-mediated polyubiquitination, decreasing substrate affinity, does not affect UBE2C-mediated multiubiquitination; when associated with K-350. | |||
Variants
![](/variants.8e7f84.jpg)
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 814 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | ||
---|---|---|---|---|---|---|
Chain | PRO_0000119811 | 1-822 | UniProt | Anaphase-promoting complex subunit 2 | ||
Modified residue | 218 | UniProt | Phosphoserine | |||
Modified residue (large scale data) | 218 | PRIDE | Phosphoserine | |||
Modified residue | 314 | UniProt | Phosphoserine | |||
Modified residue (large scale data) | 314 | PRIDE | Phosphoserine | |||
Modified residue | 470 | UniProt | Phosphoserine | |||
Modified residue (large scale data) | 470 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 532 | PRIDE | Phosphoserine | |||
Modified residue | 534 | UniProt | Phosphoserine | |||
Modified residue (large scale data) | 534 | PRIDE | Phosphoserine | |||
Modified residue | 697 | UniProt | Phosphoserine | |||
Modified residue (large scale data) | 732 | PRIDE | Phosphoserine | |||
Modified residue (large scale data) | 736 | PRIDE | Phosphoserine | |||
Modified residue | 810 | UniProt | Phosphotyrosine | |||
Modified residue (large scale data) | 810 | PRIDE | Phosphotyrosine | |||
Modified residue (large scale data) | 811 | PRIDE | Phosphoserine | |||
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5 (PubMed:25043029, PubMed:26083744).
In the context of the APC/C complex, directly interacts with UBE2C and UBE2S (PubMed:27259151).
Interacts (via cullin domain) with ANAPC11 and with UBCH10 (PubMed:11739784).
Interacts with NEUROD2 (By similarity).
Interacts with FBXO43; the interaction is direct
In the context of the APC/C complex, directly interacts with UBE2C and UBE2S (PubMed:27259151).
Interacts (via cullin domain) with ANAPC11 and with UBCH10 (PubMed:11739784).
Interacts with NEUROD2 (By similarity).
Interacts with FBXO43; the interaction is direct
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | Q9UJX6 | FBXO5 Q9UKT4-1 | 7 | EBI-396211, EBI-16059332 | |
BINARY | Q9UJX6 | MEOX1 P50221 | 3 | EBI-396211, EBI-2864512 | |
BINARY | Q9UJX6 | UBE2S Q16763 | 4 | EBI-396211, EBI-2339823 | |
BINARY | Q9UJX6 | WDR5 P61964 | 3 | EBI-396211, EBI-540834 |
Complex viewer
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 450-495 | Disordered | |||
Compositional bias | 468-481 | Acidic residues | |||
Region | 502-700 | Cullin homology | |||
Sequence similarities
Belongs to the cullin family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q9UJX6-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length822
- Mass (Da)93,828
- Last updated2000-05-01 v1
- MD5 ChecksumEAB586AFEE2BA87486C9DA5E76110FC0
Q9UJX6-2
- Name2
- Differences from canonical
- 350-352: Missing
Sequence caution
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Alternative sequence | VSP_008463 | 350-352 | in isoform 2 | ||
Compositional bias | 468-481 | Acidic residues | |||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF191337 EMBL· GenBank· DDBJ | AAF05751.1 EMBL· GenBank· DDBJ | mRNA | ||
AL929554 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC032503 EMBL· GenBank· DDBJ | AAH32503.1 EMBL· GenBank· DDBJ | mRNA | ||
BC001579 EMBL· GenBank· DDBJ | AAH01579.1 EMBL· GenBank· DDBJ | mRNA | ||
BC009487 EMBL· GenBank· DDBJ | AAH09487.2 EMBL· GenBank· DDBJ | mRNA | Sequence problems. | |
AB037827 EMBL· GenBank· DDBJ | BAA92644.1 EMBL· GenBank· DDBJ | mRNA |