UniProtKB - Q9R066 (CXAR_RAT)
Protein
Coxsackievirus and adenovirus receptor homolog
Gene
Cxadr
Organism
Rattus norvegicus (Rat)
Status
Functioni
Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair (By similarity).By similarity
GO - Molecular functioni
- beta-catenin binding Source: UniProtKB
- cell adhesion molecule binding Source: UniProtKB
- connexin binding Source: UniProtKB
- integrin binding Source: RGD
- PDZ domain binding Source: UniProtKB
GO - Biological processi
- actin cytoskeleton reorganization Source: UniProtKB
- AV node cell-bundle of His cell adhesion involved in cell communication Source: RGD
- AV node cell to bundle of His cell communication Source: UniProtKB
- cardiac muscle fiber development Source: UniProtKB
- cell-cell adhesion Source: RGD
- cell-cell junction organization Source: UniProtKB
- epithelial structure maintenance Source: UniProtKB
- gamma-delta T cell activation Source: UniProtKB
- germ cell migration Source: UniProtKB
- heart development Source: UniProtKB
- heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
- homotypic cell-cell adhesion Source: UniProtKB
- mitochondrion organization Source: UniProtKB
- negative regulation of cardiac muscle cell proliferation Source: RGD
- neutrophil chemotaxis Source: UniProtKB
- regulation of AV node cell action potential Source: RGD
- transepithelial transport Source: RGD
Keywordsi
Molecular function | Receptor |
Biological process | Cell adhesion |
Enzyme and pathway databases
Reactomei | R-RNO-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-RNO-202733, Cell surface interactions at the vascular wall |
Names & Taxonomyi
Protein namesi | Recommended name: Coxsackievirus and adenovirus receptor homologShort name: CAR Short name: rCAR |
Gene namesi | Name:Cxadr Synonyms:Car |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619794, Cxadr |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
- Basolateral cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
Other locations
- tight junction By similarity
- adherens junction By similarity
Note: In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.By similarity
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- apicolateral plasma membrane Source: UniProtKB
- basolateral plasma membrane Source: RGD
- plasma membrane Source: UniProtKB
Other locations
- acrosomal vesicle Source: UniProtKB
- adherens junction Source: UniProtKB
- bicellular tight junction Source: UniProtKB
- cell body Source: UniProtKB
- cell-cell junction Source: RGD
- cytoplasm Source: UniProtKB
- filopodium Source: UniProtKB
- growth cone Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
- intercalated disc Source: RGD
- membrane raft Source: UniProtKB
- neuron projection Source: UniProtKB
- protein-containing complex Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 238 | ExtracellularSequence analysisAdd BLAST | 219 | |
Transmembranei | 239 – 259 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 260 – 365 | CytoplasmicSequence analysisAdd BLAST | 106 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Tight junctionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000014742 | 20 – 365 | Coxsackievirus and adenovirus receptor homologAdd BLAST | 346 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 41 ↔ 120 | PROSITE-ProRule annotation | ||
Glycosylationi | 106 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 146 ↔ 223 | PROSITE-ProRule annotation | ||
Disulfide bondi | 162 ↔ 212 | PROSITE-ProRule annotation | ||
Lipidationi | 259 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 260 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 297 | PhosphoserineBy similarity | 1 | |
Modified residuei | 304 | PhosphoserineBy similarity | 1 | |
Modified residuei | 306 | PhosphoserineBy similarity | 1 | |
Modified residuei | 323 | PhosphoserineCombined sources | 1 | |
Modified residuei | 332 | PhosphoserineCombined sources | 1 | |
Modified residuei | 363 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
N-glycosylated.By similarity
Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | Q9R066 |
PRIDEi | Q9R066 |
PTM databases
GlyGeni | Q9R066, 1 site |
iPTMneti | Q9R066 |
PhosphoSitePlusi | Q9R066 |
SwissPalmi | Q9R066 |
Expressioni
Tissue specificityi
Expressed in heart, brain, spleen, lung, liver, muscle, kidney, testis, spleen and skeletal muscle.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000001557, Expressed in jejunum and 20 other tissues |
Genevisiblei | Q9R066, RN |
Interactioni
Subunit structurei
Monomer. May form homodimer.
Interacts with LNX, MAGI1, DLG4, PRKCABP, TJP1 and CTNNB1.
Interacts with MPDZ; recruits MPDZ to intercellular contact sites.
Interacts with JAML (homodimeric form) (By similarity).
By similarityGO - Molecular functioni
- beta-catenin binding Source: UniProtKB
- cell adhesion molecule binding Source: UniProtKB
- connexin binding Source: UniProtKB
- integrin binding Source: RGD
- PDZ domain binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000041873 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 136 | Ig-like C2-type 1Add BLAST | 117 | |
Domaini | 141 – 228 | Ig-like C2-type 2Add BLAST | 88 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 360 – 365 | PDZ-bindingBy similarity | 6 |
Domaini
The Ig-like C2-type 1 domain mediates homodimerization and interaction with JAML.By similarity
The PDZ-binding motif mediates interaction with MPDZ and MAGI1.By similarity
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QSG0, Eukaryota |
GeneTreei | ENSGT00940000154829 |
HOGENOMi | CLU_040549_0_0_1 |
InParanoidi | Q9R066 |
OMAi | ICNEIRE |
OrthoDBi | 896005at2759 |
PhylomeDBi | Q9R066 |
TreeFami | TF330875 |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR003598, Ig_sub2 IPR013106, Ig_V-set IPR013151, Immunoglobulin |
Pfami | View protein in Pfam PF00047, ig, 1 hit PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 2 hits SM00408, IGc2, 2 hits SM00406, IGv, 1 hit |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9R066-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALLLCFVLL CGVADFTSSL SITTPEQRIE KAKGETAYLP CKFTLEPEDQ
60 70 80 90 100
GPLDIEWLIS PSDNQKVDQV IILYSGDKIY DNYYPDLKGR VHFTSNDVKS
110 120 130 140 150
GDASINVTNL QLSDIGTYQC KVKKAPGVAN RKFLLTVLVK PSGTRCFVDG
160 170 180 190 200
SGEIGNDFKL KCEPKEGSLP LQYEWQKLSD SQKMPTPWLA EMTSPVISVK
210 220 230 240 250
NASSEYSGTY SCTVQNRVGS DQCMLRLDVV PPSNRAGTIA GAVIGTLLAL
260 270 280 290 300
VLIGAILFCC HKKRREEKYE KEVHHDIRED VPPPKSRTST ARSYIGSNHS
310 320 330 340 350
SLGSMSPSNM EGYSKTQYNQ VPSEDFERAP QSPTLAPAKV AAPNLSRMGA
360
VPVMIPAQSK DGSIV
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_014815 | 340 – 352 | VAAPN…MGAVP → FKYAYLTDGIAVV in isoform 2. CuratedAdd BLAST | 13 | |
Alternative sequenceiVSP_014816 | 353 – 365 | Missing in isoform 2. CuratedAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AABR03078554 Genomic DNA No translation available. AABR03079390 Genomic DNA No translation available. AF109643 mRNA Translation: AAF01254.1 AF109644 mRNA Translation: AAF01255.1 BC088313 mRNA Translation: AAH88313.1 |
RefSeqi | NP_446022.1, NM_053570.1 [Q9R066-2] XP_006248062.1, XM_006248000.3 [Q9R066-1] |
Genome annotation databases
Ensembli | ENSRNOT00000050701; ENSRNOP00000041873; ENSRNOG00000001557 [Q9R066-2] |
GeneIDi | 89843 |
KEGGi | rno:89843 |
UCSCi | RGD:619794, rat [Q9R066-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AABR03078554 Genomic DNA No translation available. AABR03079390 Genomic DNA No translation available. AF109643 mRNA Translation: AAF01254.1 AF109644 mRNA Translation: AAF01255.1 BC088313 mRNA Translation: AAH88313.1 |
RefSeqi | NP_446022.1, NM_053570.1 [Q9R066-2] XP_006248062.1, XM_006248000.3 [Q9R066-1] |
3D structure databases
SMRi | Q9R066 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000041873 |
PTM databases
GlyGeni | Q9R066, 1 site |
iPTMneti | Q9R066 |
PhosphoSitePlusi | Q9R066 |
SwissPalmi | Q9R066 |
Proteomic databases
PaxDbi | Q9R066 |
PRIDEi | Q9R066 |
Genome annotation databases
Ensembli | ENSRNOT00000050701; ENSRNOP00000041873; ENSRNOG00000001557 [Q9R066-2] |
GeneIDi | 89843 |
KEGGi | rno:89843 |
UCSCi | RGD:619794, rat [Q9R066-1] |
Organism-specific databases
CTDi | 1525 |
RGDi | 619794, Cxadr |
Phylogenomic databases
eggNOGi | ENOG502QSG0, Eukaryota |
GeneTreei | ENSGT00940000154829 |
HOGENOMi | CLU_040549_0_0_1 |
InParanoidi | Q9R066 |
OMAi | ICNEIRE |
OrthoDBi | 896005at2759 |
PhylomeDBi | Q9R066 |
TreeFami | TF330875 |
Enzyme and pathway databases
Reactomei | R-RNO-198933, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-RNO-202733, Cell surface interactions at the vascular wall |
Miscellaneous databases
PROi | PR:Q9R066 |
Gene expression databases
Bgeei | ENSRNOG00000001557, Expressed in jejunum and 20 other tissues |
Genevisiblei | Q9R066, RN |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR003598, Ig_sub2 IPR013106, Ig_V-set IPR013151, Immunoglobulin |
Pfami | View protein in Pfam PF00047, ig, 1 hit PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 2 hits SM00408, IGc2, 2 hits SM00406, IGv, 1 hit |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CXAR_RAT | |
Accessioni | Q9R066Primary (citable) accession number: Q9R066 Secondary accession number(s): Q5M817, Q9R067 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | July 19, 2005 | |
Last modified: | April 7, 2021 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |