Q9QXV8 · SPY2_MOUSE

  • Protein
    Protein sprouty homolog 2
  • Gene
    Spry2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Antagonist of fibroblast growth factor (FGF) pathways via inhibition of FGF-mediated phosphorylation of ERK1/2 (PubMed:29501879).
Thereby acts as an antagonist of FGF-induced retinal lens fiber differentiation, may inhibit limb bud outgrowth and may negatively modulate respiratory organogenesis (PubMed:10074434, PubMed:10498682, PubMed:29501879).
Inhibits TGFB-induced epithelial-to-mesenchymal transition in retinal lens epithelial cells (PubMed:25576668).
Inhibits CBL/C-CBL-mediated EGFR ubiquitination (By similarity).

Features

Showing features for site.

131520406080100120140160180200220240260280300
TypeIDPosition(s)Description
Site143-144Cleavage; by FAP

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentactin cytoskeleton
Cellular Componentcytoskeleton
Cellular Componentcytosol
Cellular Componentmembrane
Cellular Componentmicrotubule end
Cellular Componentnucleus
Cellular Componentruffle membrane
Molecular Functionmolecular function inhibitor activity
Molecular Functionprotein kinase binding
Molecular Functionprotein serine/threonine kinase activator activity
Molecular Functionubiquitin-protein transferase inhibitor activity
Biological Processbranching morphogenesis of an epithelial tube
Biological Processbud elongation involved in lung branching
Biological Processcell fate commitment
Biological Processcellular response to leukemia inhibitory factor
Biological Processcellular response to vascular endothelial growth factor stimulus
Biological ProcessERK1 and ERK2 cascade
Biological Processestablishment of mitotic spindle orientation
Biological Processfibroblast growth factor receptor signaling pathway
Biological Processinner ear morphogenesis
Biological Processlung development
Biological Processlung growth
Biological Processlung morphogenesis
Biological Processnegative regulation of angiogenesis
Biological Processnegative regulation of apoptotic process
Biological Processnegative regulation of cell population proliferation
Biological Processnegative regulation of cell projection organization
Biological Processnegative regulation of epithelial to mesenchymal transition
Biological Processnegative regulation of ERK1 and ERK2 cascade
Biological Processnegative regulation of fibroblast growth factor receptor signaling pathway
Biological Processnegative regulation of lens fiber cell differentiation
Biological Processnegative regulation of neurotrophin TRK receptor signaling pathway
Biological Processnegative regulation of peptidyl-threonine phosphorylation
Biological Processnegative regulation of protein ubiquitination
Biological Processnegative regulation of Ras protein signal transduction
Biological Processnegative regulation of transforming growth factor beta receptor signaling pathway
Biological Processnegative regulation of vascular endothelial growth factor signaling pathway
Biological Processpositive regulation of cell migration
Biological Processpositive regulation of epidermal growth factor receptor signaling pathway
Biological Processpositive regulation of ERK1 and ERK2 cascade
Biological Processpositive regulation of gene expression
Biological Processpositive regulation of peptidyl-serine phosphorylation
Biological Processpositive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
Biological Processrespiratory system development
Biological Processsensory perception of sound

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein sprouty homolog 2
  • Short names
    Spry-2

Gene names

    • Name
      Spry2

Organism names

  • Taxonomic identifier
  • Strain
    • Swiss Webster
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q9QXV8
  • Secondary accessions
    • Q9WUQ9

Proteomes

Organism-specific databases

Subcellular Location

Note: Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis55Abolishes FGF2-induced lens fiber differentiation via inhibition of FGF-mediated ERK1/2 phosphorylation.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 13 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000769021-315Protein sprouty homolog 2

Post-translational modification

Cleaved at Pro-143 by the prolyl endopeptidase FAP (seprase) activity (in vitro).

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in the testes and brain (at protein level) (PubMed:10074434, PubMed:17974561).
In adult, highly expressed in the lung, heart and at lower levels in skeletal muscle and kidney (PubMed:10074434).

Developmental stage

At 8.5 dpc, expressed in the primitive streak, rostral forebrain, cells lateral to the posterior hindbrain, anterior hindbrain and developing midbrain. At 9.5 dpc, continues to be expressed in the rostral forebrain and primitive streak, and is also detected in the branchial arches and the forelimb bud. At 10.5 dpc, expressed in the somites, frontonasal processes, tailbud, and hindlimb bud (PubMed:10498682).
Highly expressed in lung epithelial cells, primarily in the distal airways at 12 dpc (PubMed:10074434).

Gene expression databases

Interaction

Subunit

Forms heterodimers with SPRY1 (PubMed:16877379).
Forms a tripartite complex containing GAB1, METTL13 and SPRY2 (By similarity).
Within the complex interacts with METTL13 (By similarity).
Interacts with RAF1 (By similarity).
Interacts (via C-terminus) with TESK1 (via C-terminus); the interaction disrupts SPRY2 interaction with GRB2, potentially via disruption of SPRY2 serine dephosphorylation (PubMed:17974561).
Interacts with PPP2R1A/PP2A-A and PPP2CA/PP2A-C; the interaction with PPP2CA/PP2A-C is inhibited by interaction with TESK1, possibly by vesicular sequestration of SPRY2 (By similarity).
Inhibition of the interaction with the serine/threonine-protein phosphatase 2A (PP2A) holoenzyme results in loss of PP2A-mediated dephosphorylation, resulting in the loss of SPRY2 interaction with GRB2 (By similarity).
Interacts with GRB2 (By similarity).
Interacts with CBL/C-CBL; the interaction inhibits CBL-mediated ubiquitination of EGFR (By similarity).
Interacts (via C-terminus) with CAV1 (via C-terminus) (PubMed:16877379).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for compositional bias, region, domain.

TypeIDPosition(s)Description
Compositional bias1-19Polar residues
Region1-39Disordered
Region51-141Disordered
Compositional bias73-88Basic and acidic residues
Compositional bias94-136Polar residues
Region117-315Required for interaction with CAV1
Domain177-291SPR
Region178-315Required for interaction with TESK1

Domain

The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.

Sequence similarities

Belongs to the sprouty family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    315
  • Mass (Da)
    34,623
  • Last updated
    2000-05-01 v1
  • Checksum
    81514698EAD809A7
MEARAQSGNGSQPLLQTAHDSGRQRGEPDPRDALTQQVHVLSLDQIRAIRNTNEYTEGPTVVPRPGLKPAPRPSTQHKHERLHGLPEHRQPPRLQPSQVHSSRAPLSRSISTVSSGSRSSTRTSTSSSSSEQRLLGPSFSHGPAAADGIIRVQPKSELKPGDVKPLSKDDLGLHAYRCEDCGKCKCKECTYPRPLPSDWICDKQCLCSAQNVIDYGTCVCCVKGLFYHCSNDDEDNCADNPCSCSQSHCCTRWSAMGVMSLFLPCLWCYLPAKGCLKLCQGCYDRVNRPGCRCKNSNTVCCKVPTVPPRNFEKPT

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias1-19Polar residues
Compositional bias73-88Basic and acidic residues
Compositional bias94-136Polar residues
Sequence conflict163in Ref. 2; AAD34167

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF176905
EMBL· GenBank· DDBJ
AAD56006.1
EMBL· GenBank· DDBJ
mRNA
AF153084
EMBL· GenBank· DDBJ
AAD34167.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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