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Q9FA38 · FAE_METEA

Function

function

Catalyzes the condensation of formaldehyde with tetrahydromethanopterin (H4MPT) to 5,10-methylenetetrahydromethanopterin, a reaction which also proceeds spontaneously, but at a lower rate than that of the enzyme-catalyzed reaction. Is an essential enzyme for methylotrophic energy metabolism and formaldehyde detoxification of this bacterium.

Catalytic activity

Activity regulation

Inhibited by the addition of an excess of H4F.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.2 mMformaldehyde
The KM for H4MPT is higher than 60 uM.

pH Dependence

Optimum pH is 7-7.5.

Pathway

One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (H4MPT route): step 1/5.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site22Proton donor
Binding site24substrate
Binding site53substrate
Binding site71substrate
Binding site73substrate
Binding site88substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functioncarbon-nitrogen lyase activity
Biological Processcarbohydrate biosynthetic process
Biological Processformaldehyde catabolic process
Biological Processone-carbon metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    5,6,7,8-tetrahydromethanopterin hydro-lyase
  • EC number
  • Alternative names
    • Formaldehyde-activating enzyme (Fae)

Gene names

    • Name
      fae
    • Ordered locus names
      MexAM1_META1p1766

Organism names

Accessions

  • Primary accession
    Q9FA38
  • Secondary accessions
    • C5B145

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene are unable to grow on methanol but exhibit wild-type growth on succinate. In addition, mutant is shown to be formaldehyde sensitive.

Chemistry

PTM/Processing

Features

Showing features for initiator methionine, chain.

Type
IDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00000871712-1705,6,7,8-tetrahydromethanopterin hydro-lyase

Interaction

Subunit

Homopentamer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    170
  • Mass (Da)
    18,091
  • Last updated
    2007-01-23 v3
  • MD5 Checksum
    1C6D98D9523DB49B4506C5FF11837EF7
MAKITKVQVGEALVGDGNEVAHIDLIIGPRGSPAETAFCNGLVNNKHGFTSLLAVIAPNLPCKPNTLMFNKVTINDARQAVQMFGPAQHGVAMAVQDAVAEGIIPADEADDLYVLVGVFIHWEAADDAKIQKYNYEATKLSIQRAVNGEPKASVVTEQRKSATHPFAANA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF032114
EMBL· GenBank· DDBJ
AAG32954.1
EMBL· GenBank· DDBJ
Genomic DNA
CP001510
EMBL· GenBank· DDBJ
ACS39609.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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