Q9FA38 · FAE_METEA
- Protein5,6,7,8-tetrahydromethanopterin hydro-lyase
- Genefae
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids170 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the condensation of formaldehyde with tetrahydromethanopterin (H4MPT) to 5,10-methylenetetrahydromethanopterin, a reaction which also proceeds spontaneously, but at a lower rate than that of the enzyme-catalyzed reaction. Is an essential enzyme for methylotrophic energy metabolism and formaldehyde detoxification of this bacterium.
Catalytic activity
- 5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10-methylenetetrahydromethanopterin + H2O
Activity regulation
Inhibited by the addition of an excess of H4F.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.2 mM | formaldehyde |
The KM for H4MPT is higher than 60 uM.
pH Dependence
Optimum pH is 7-7.5.
Pathway
One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (H4MPT route): step 1/5.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 22 | Proton donor | |||
Binding site | 24 | substrate | |||
Binding site | 53 | substrate | |||
Binding site | 71 | substrate | |||
Binding site | 73 | substrate | |||
Binding site | 88 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | carbon-nitrogen lyase activity | |
Biological Process | carbohydrate biosynthetic process | |
Biological Process | formaldehyde catabolic process | |
Biological Process | one-carbon metabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5,6,7,8-tetrahydromethanopterin hydro-lyase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Methylobacteriaceae > Methylorubrum
Accessions
- Primary accessionQ9FA38
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Cells lacking this gene are unable to grow on methanol but exhibit wild-type growth on succinate. In addition, mutant is shown to be formaldehyde sensitive.
Chemistry
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Initiator methionine | 1 | Removed | |||
Chain | PRO_0000087171 | 2-170 | 5,6,7,8-tetrahydromethanopterin hydro-lyase | ||
Interaction
Structure
Sequence
- Sequence statusComplete
- Length170
- Mass (Da)18,091
- Last updated2007-01-23 v3
- MD5 Checksum1C6D98D9523DB49B4506C5FF11837EF7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF032114 EMBL· GenBank· DDBJ | AAG32954.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP001510 EMBL· GenBank· DDBJ | ACS39609.1 EMBL· GenBank· DDBJ | Genomic DNA |