Q93W57 · HSDA_SESIN
- Protein11-beta-hydroxysteroid dehydrogenase A
- GeneSOP2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids348 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Has dehydrogenase activity against corticosterone (11 beta-hydroxysteroid) and estradiol (17 beta-hydroxysteroid), with higher activity against estradiol. Possesses higher dehydrogenase activity with NADP+ than NAD+ regardless of the sterol substrate. May be involved in signal transduction regulated by various sterols.
Catalytic activity
- an 11beta-hydroxysteroid + NADP+ = an 11-oxosteroid + NADPH + H+
- an 11beta-hydroxysteroid + NAD+ = an 11-oxosteroid + NADH + H+
- corticosterone + NADP+ = 11-dehydrocorticosterone + NADPH + H+
- corticosterone + NAD+ = 11-dehydrocorticosterone + NADH + H+
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 54-80 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: GASSGIGESLAYEYAKRGACLVLAARR | ||||||
Binding site | 105 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 132-135 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: NAGI | ||||||
Binding site | 184 | substrate | ||||
Sequence: S | ||||||
Active site | 197 | Proton acceptor | ||||
Sequence: Y | ||||||
Binding site | 197-201 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: YNASK | ||||||
Binding site | 201 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | lipid droplet | |
Cellular Component | membrane | |
Molecular Function | 11-beta-hydroxysteroid dehydrogenase (NAD+) activity | |
Molecular Function | 11-beta-hydroxysteroid dehydrogenase (NADP+) activity | |
Molecular Function | 17-beta-hydroxysteroid dehydrogenase (NADP+) activity | |
Molecular Function | estradiol 17-beta-dehydrogenase [NAD(P)+] activity | |
Biological Process | steroid metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name11-beta-hydroxysteroid dehydrogenase A
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Lamiales > Pedaliaceae > Sesamum
Accessions
- Primary accessionQ93W57
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass type II membrane protein
Note: Surface of oil bodies. Exists at a monolayer lipid/water interface.
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 10-30 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: LIAPPFTFFFLLFFLPPFQIF |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000449958 | 1-348 | 11-beta-hydroxysteroid dehydrogenase A | |||
Sequence: MDLIHTFLNLIAPPFTFFFLLFFLPPFQIFKFFLSILGTLFSEDVAGKVVVITGASSGIGESLAYEYAKRGACLVLAARRERSLQEVAERARDLGSPDVVVVRADVSKAEDCRKVVDQTMNRFGRLDHLVNNAGIMSVSMLEEVEDITGYRETMDINFWGYVYMTRFAAPYLRNSRGRIVVLSSSSSWMPTPRMSFYNASKAAISQFFETLRVEFGPDIGITLVTPGFIESELTQGKFYNAGERVIDQDMRDVQVSTTPILRVESAARSIVRSAIRGERYVTEPAWFRVTYWWKLFCPEVMEWVFRLMYLASPGEPEKETFGKKVLDYTGVKSLLYPETVQVPEPKND |
Expression
Tissue specificity
Expressed in seeds (at protein level) (PubMed:11950969, PubMed:9816677).
Not expressed in stem, leaf or root (at protein level) (PubMed:9816677).
Not expressed in stem, leaf or root (at protein level) (PubMed:9816677).
Developmental stage
Expressed during seed maturation (PubMed:11950969, PubMed:15331088, PubMed:9816677).
Appears in maturing seeds approximately 3 weeks after flowering and thereafter the level is continuously increased till the late stage of seed maturation (at protein level) (PubMed:15331088).
Detected in maturing seeds approximately 2 weeks after flowering and expression is maintained at a substantial level thereafter until the late stage of seed maturation (PubMed:11950969).
Appears in maturing seeds approximately 3 weeks after flowering and thereafter the level is continuously increased till the late stage of seed maturation (at protein level) (PubMed:15331088).
Detected in maturing seeds approximately 2 weeks after flowering and expression is maintained at a substantial level thereafter until the late stage of seed maturation (PubMed:11950969).
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 13-26 | Proline-knob | ||||
Sequence: PPFTFFFLLFFLPP |
Domain
The proline-knob motif may be involved in the targeting to oil bodies.
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length348
- Mass (Da)39,568
- Last updated2001-12-01 v1
- Checksum53A0C6DF804CB59B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF302806 EMBL· GenBank· DDBJ | AAL09328.1 EMBL· GenBank· DDBJ | mRNA | ||
AF421889 EMBL· GenBank· DDBJ | AAL13315.1 EMBL· GenBank· DDBJ | Genomic DNA |