Q7A3E1 · CRTM_STAAN
- Protein4,4'-diapophytoene synthase
- GenecrtM
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids287 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the biosynthesis of the yellow-orange carotenoid staphyloxanthin, which plays a role in the virulence via its protective function against oxidative stress. Catalyzes the head-to-head condensation of two molecules of farnesyl diphosphate (FPP) into the colorless C30 carotenoid 4,4'-diapophytoene (dehydrosqualene).
Catalytic activity
- 2 (2E,6E)-farnesyl diphosphate = 15-cis-4,4'-diapophytoene + 2 diphosphate
Cofactor
Note: Binds 2 Mg2+ ions per subunit.
Pathway
Carotenoid biosynthesis; staphyloxanthin biosynthesis; staphyloxanthin from farnesyl diphosphate: step 1/5.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 18-21 | (2E,6E)-farnesyl diphosphate 1 (UniProtKB | ChEBI) | ||||
Sequence: HSKS | ||||||
Binding site | 41 | (2E,6E)-farnesyl diphosphate 1 (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 45 | (2E,6E)-farnesyl diphosphate 1 (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 45 | (2E,6E)-farnesyl diphosphate 2 (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 48 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 52 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 165 | (2E,6E)-farnesyl diphosphate 2 (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 168 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 171 | (2E,6E)-farnesyl diphosphate 1 (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 172 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 248 | (2E,6E)-farnesyl diphosphate 1 (UniProtKB | ChEBI) | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | farnesyl-diphosphate farnesyltransferase activity | |
Molecular Function | farnesyltranstransferase activity | |
Molecular Function | geranylgeranyl-diphosphate geranylgeranyltransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | carotenoid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name4,4'-diapophytoene synthase
- EC number
- Short namesDAP synthase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionQ7A3E1
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000282621 | 1-287 | 4,4'-diapophytoene synthase | |||
Sequence: MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVCRKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQSFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRLGESLQLINILRDVGEDFDNERVYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAIAEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKLFHEINSKYHRI |
Structure
Sequence
- Sequence statusComplete
- Length287
- Mass (Da)34,217
- Last updated2004-07-05 v1
- ChecksumE5CE311E3B76A452
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000018 EMBL· GenBank· DDBJ | BAB43653.1 EMBL· GenBank· DDBJ | Genomic DNA |