Q642A5 · DPOE3_RAT
- ProteinDNA polymerase epsilon subunit 3
- GenePole3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids145 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Accessory component of the DNA polymerase epsilon complex (By similarity).
Participates in DNA repair and in chromosomal DNA replication (By similarity).
Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1 (By similarity).
Does not enhance nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex (By similarity).
Participates in DNA repair and in chromosomal DNA replication (By similarity).
Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1 (By similarity).
Does not enhance nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | ATAC complex | |
Cellular Component | CHRAC | |
Cellular Component | epsilon DNA polymerase complex | |
Cellular Component | pericentric heterochromatin | |
Molecular Function | chromatin DNA binding | |
Molecular Function | protein heterodimerization activity | |
Biological Process | chromatin remodeling | |
Biological Process | DNA damage response | |
Biological Process | DNA-templated DNA replication | |
Biological Process | heterochromatin formation | |
Biological Process | leading strand elongation | |
Biological Process | negative regulation of transcription by RNA polymerase II | |
Biological Process | nucleosome assembly | |
Biological Process | regulation of DNA replication |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA polymerase epsilon subunit 3
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ642A5
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylalanine | ||||
Sequence: A | ||||||
Chain | PRO_0000208344 | 2-145 | DNA polymerase epsilon subunit 3 | |||
Sequence: AERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTLNASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKDCEEQDKSREEEDEDEERLDEEEQNEEEEVDN | ||||||
Modified residue | 83 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 121 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Component of the DNA polymerase epsilon complex consisting of four subunits: the catalytic subunit POLE and the accessory subunits POLE2, POLE3 and POLE4. Interaction with POLE4 is a prerequisite for further binding with POLE and POLE2. Heterodimer with CHRAC1; binds to DNA (By similarity).
Component of the CHRAC ISWI chromatin remodeling complex at least composed of SMARCA5/SNF2H, BAZ1A/ACF1, CHRAC1 and POLE3; the complex preferentially binds DNA through the CHRAC1-POLE3 heterodimer and possesses ATP-dependent nucleosome-remodeling activity (By similarity).
Within the complex, the heterodimer with CHRAC1 interacts with SMARCA5/SNF2H; the interaction is direct and enhances nucleosome sliding activity by the SMARCA5/SNF2H and BAZ1A/ACF1 interaction (By similarity).
Within the complex, the heterodimer with CHRAC1 interacts with BAZ1A/ACF1; the interactions are direct (By similarity).
Component of the CHRAC ISWI chromatin remodeling complex at least composed of SMARCA5/SNF2H, BAZ1A/ACF1, CHRAC1 and POLE3; the complex preferentially binds DNA through the CHRAC1-POLE3 heterodimer and possesses ATP-dependent nucleosome-remodeling activity (By similarity).
Within the complex, the heterodimer with CHRAC1 interacts with SMARCA5/SNF2H; the interaction is direct and enhances nucleosome sliding activity by the SMARCA5/SNF2H and BAZ1A/ACF1 interaction (By similarity).
Within the complex, the heterodimer with CHRAC1 interacts with BAZ1A/ACF1; the interactions are direct (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for coiled coil, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Coiled coil | 85-144 | |||||
Sequence: LKEALEAYRREQKGKKEASEQKKKDKDKKDCEEQDKSREEEDEDEERLDEEEQNEEEEVD | ||||||
Compositional bias | 93-119 | Basic and acidic residues | ||||
Sequence: RREQKGKKEASEQKKKDKDKKDCEEQD | ||||||
Region | 93-145 | Disordered | ||||
Sequence: RREQKGKKEASEQKKKDKDKKDCEEQDKSREEEDEDEERLDEEEQNEEEEVDN | ||||||
Compositional bias | 120-145 | Acidic residues | ||||
Sequence: KSREEEDEDEERLDEEEQNEEEEVDN |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length145
- Mass (Da)16,674
- Last updated2004-10-25 v1
- Checksum6CB14ED20FE15EC1
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I6AK16 | A0A8I6AK16_RAT | Pole3 | 157 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 93-119 | Basic and acidic residues | ||||
Sequence: RREQKGKKEASEQKKKDKDKKDCEEQD | ||||||
Compositional bias | 120-145 | Acidic residues | ||||
Sequence: KSREEEDEDEERLDEEEQNEEEEVDN |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC081988 EMBL· GenBank· DDBJ | AAH81988.1 EMBL· GenBank· DDBJ | mRNA | ||
BC083800 EMBL· GenBank· DDBJ | AAH83800.1 EMBL· GenBank· DDBJ | mRNA |