Q63272 · JAK3_RAT
- ProteinTyrosine-protein kinase JAK3
- GeneJak3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1100 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A and STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion (By similarity).
Catalytic activity
- L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H+
Features
Showing features for binding site, active site.
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTyrosine-protein kinase JAK3
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ63272
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endomembrane system ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000088117 | 1-1100 | Tyrosine-protein kinase JAK3 | |||
Sequence: MAPPSEETPLISQRSCSLSSSEAGALHVLLPPRGPGPPQRLSFSFGDYLAEDLCVRAAKACGILPVYHSLFALATEDLSCWFPPSHIFSIEDVDTQVLVYRLRFYFPGWFGLETCHRFGLHKDLTSAILDVHVLEHLFAQHRSDLVSGRLPVGLSLKDQGEFLSLAVLDLAQMARKQAQRPGELLKSVSYKACLPPSLRDLIQGQSFVTRRRIRRTVVQALRRVVACQADRYALMAKYILDLERLHPAATTESFLVGLPGAQEEPGCLRVTGDNGIAWSSKDQELFQTFCDFPEIVDVSIKQAPRVGPAGEHRLVTITRMDGHILEAEFPGLPEALSFVALVDGYFRLICDSRHFFCKEVAPPRLLEEEAELCHGPITLDFAIHKLKAAGSLPGSYILRRSPQDYDSFLLTACVQTPLGPDYKGCLIRQDPSGAFSLVGLSQLHRSLQELLTACWHSGLQVDGTALNLTSCCVPRPKEKSNLIVVRRGRNPTPAPGHSPSCCALTKLSFHTIPADSLEWHENLGHGSFTKIFHGHRREVVDGETHDTEVLLKVMDSRHQNCMESFLEAASLMSQVSYPHLVLLHGVCMAGDSIMVQEFVYLGAIDTYLRKRGHLVPASWKLQVTKQLAYALNYLEDKGLPHGNVSARKVLLAREGVDGNPPFIKLSDPGVSPTVLSLEMLTDRIPWVAPECLQEAGTLNLEADKWGFGATTWEVFSGAPMHITSLEPAKKLKFYEDRGQLPALKWTELEGLIAQCMAYDPGRRPSFRAILRDLNGLITSDYELLSDPTPGIPNPRDELCGGAQLYACQDPAIFEERHLKYISLLGKGNFGSVELCRYDPLGDNTGPLVAVKQLQHSGPEQQRDFQREIQILKALHCDFIVKYRGVSYGPGRQSLRLVMEYLPSGCLRDFLQRHRARLHNDRLLLFAWQICKGMEYLGARRCVHRDLAARNILVESEAHVKIADFGLAKLLPLGKDYYVVREPGQSPIFWYAPESLSDNIFSRQSDVWSFGVVLYELFTYSDKSCSPSTEFLRMMGPEREGSPLCHLLELLAEGRRLPPPSTCPTEVQELMQLCWSPNPQDRPAFDTLSPQLDALWRGSPG | ||||||
Modified residue | 17 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 781 | Phosphotyrosine; by autocatalysis | ||||
Sequence: Y | ||||||
Modified residue | 900 | Phosphotyrosine | ||||
Sequence: Y | ||||||
Modified residue | 935 | Phosphotyrosine | ||||
Sequence: Y | ||||||
Modified residue | 976 | Phosphotyrosine; by autocatalysis | ||||
Sequence: Y | ||||||
Modified residue | 977 | Phosphotyrosine; by autocatalysis | ||||
Sequence: Y |
Post-translational modification
Autophosphorylated, leading to regulate its activity. IL2 promotes phosphorylation on tyrosine residues, including autophosphorylation on Tyr-781 (By similarity).
Dephosphorylation of Tyr-976 and Tyr-977 by PTPN2 negatively regulates cytokine-mediated signaling (By similarity).
Dephosphorylation of Tyr-976 and Tyr-977 by PTPN2 negatively regulates cytokine-mediated signaling (By similarity).
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
In contrast with the ubiquitous expression of the other JAKs, JAK3 is predominantly expressed in hematopoietic tissues.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-223 | cytokine/interferon/growth hormone receptors | ||||
Sequence: MAPPSEETPLISQRSCSLSSSEAGALHVLLPPRGPGPPQRLSFSFGDYLAEDLCVRAAKACGILPVYHSLFALATEDLSCWFPPSHIFSIEDVDTQVLVYRLRFYFPGWFGLETCHRFGLHKDLTSAILDVHVLEHLFAQHRSDLVSGRLPVGLSLKDQGEFLSLAVLDLAQMARKQAQRPGELLKSVSYKACLPPSLRDLIQGQSFVTRRRIRRTVVQALRR | ||||||
Domain | 24-353 | FERM | ||||
Sequence: GALHVLLPPRGPGPPQRLSFSFGDYLAEDLCVRAAKACGILPVYHSLFALATEDLSCWFPPSHIFSIEDVDTQVLVYRLRFYFPGWFGLETCHRFGLHKDLTSAILDVHVLEHLFAQHRSDLVSGRLPVGLSLKDQGEFLSLAVLDLAQMARKQAQRPGELLKSVSYKACLPPSLRDLIQGQSFVTRRRIRRTVVQALRRVVACQADRYALMAKYILDLERLHPAATTESFLVGLPGAQEEPGCLRVTGDNGIAWSSKDQELFQTFCDFPEIVDVSIKQAPRVGPAGEHRLVTITRMDGHILEAEFPGLPEALSFVALVDGYFRLICDSR | ||||||
Domain | 372-472 | SH2; atypical | ||||
Sequence: LCHGPITLDFAIHKLKAAGSLPGSYILRRSPQDYDSFLLTACVQTPLGPDYKGCLIRQDPSGAFSLVGLSQLHRSLQELLTACWHSGLQVDGTALNLTSCC | ||||||
Domain | 517-777 | Protein kinase 1 | ||||
Sequence: LEWHENLGHGSFTKIFHGHRREVVDGETHDTEVLLKVMDSRHQNCMESFLEAASLMSQVSYPHLVLLHGVCMAGDSIMVQEFVYLGAIDTYLRKRGHLVPASWKLQVTKQLAYALNYLEDKGLPHGNVSARKVLLAREGVDGNPPFIKLSDPGVSPTVLSLEMLTDRIPWVAPECLQEAGTLNLEADKWGFGATTWEVFSGAPMHITSLEPAKKLKFYEDRGQLPALKWTELEGLIAQCMAYDPGRRPSFRAILRDLNGLI | ||||||
Domain | 818-1091 | Protein kinase 2 | ||||
Sequence: LKYISLLGKGNFGSVELCRYDPLGDNTGPLVAVKQLQHSGPEQQRDFQREIQILKALHCDFIVKYRGVSYGPGRQSLRLVMEYLPSGCLRDFLQRHRARLHNDRLLLFAWQICKGMEYLGARRCVHRDLAARNILVESEAHVKIADFGLAKLLPLGKDYYVVREPGQSPIFWYAPESLSDNIFSRQSDVWSFGVVLYELFTYSDKSCSPSTEFLRMMGPEREGSPLCHLLELLAEGRRLPPPSTCPTEVQELMQLCWSPNPQDRPAFDTLSPQL |
Domain
Possesses two phosphotransferase domains. The second one contains the catalytic domain, while the presence of a pseudokinase domain is required for suppression of basal activity of JAK3.
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,100
- Mass (Da)122,726
- Last updated2021-02-10 v2
- ChecksumF7FEBF8D91061C8A
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0G2K636 | A0A0G2K636_RAT | Jak3 | 1099 | ||
A0A8L2UK66 | A0A8L2UK66_RAT | Jak3 | 1097 |
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 893 | in Ref. 1; BAA05868 | ||||
Sequence: S → E | ||||||
Sequence conflict | 981 | in Ref. 1; BAA05868 | ||||
Sequence: E → V | ||||||
Sequence conflict | 1034 | in Ref. 1; BAA05868 | ||||
Sequence: M → I |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D28508 EMBL· GenBank· DDBJ | BAA05868.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AABR07024869 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH474031 EMBL· GenBank· DDBJ | EDL90763.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH474031 EMBL· GenBank· DDBJ | EDL90761.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH474031 EMBL· GenBank· DDBJ | EDL90762.1 EMBL· GenBank· DDBJ | Genomic DNA |