Q63272 · JAK3_RAT

  • Protein
    Tyrosine-protein kinase JAK3
  • Gene
    Jak3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A and STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion (By similarity).

Catalytic activity

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site824-832ATP (UniProtKB | ChEBI)
Binding site851ATP (UniProtKB | ChEBI)
Active site945Proton acceptor

GO annotations

AspectTerm
Cellular Componentcytoskeleton
Cellular Componentcytosol
Cellular Componentendomembrane system
Cellular Componentextrinsic component of cytoplasmic side of plasma membrane
Molecular FunctionATP binding
Molecular Functiongrowth hormone receptor binding
Molecular Functionnon-membrane spanning protein tyrosine kinase activity
Molecular Functionprotein phosphatase binding
Molecular Functionprotein tyrosine kinase activity
Biological Processadaptive immune response
Biological ProcessB cell differentiation
Biological Processcell differentiation
Biological Processcell surface receptor signaling pathway via JAK-STAT
Biological Processcellular response to cytokine stimulus
Biological Processcytokine-mediated signaling pathway
Biological Processenzyme-linked receptor protein signaling pathway
Biological Processgrowth hormone receptor signaling pathway via JAK-STAT
Biological Processinnate immune response
Biological Processinterleukin-15-mediated signaling pathway
Biological Processinterleukin-2-mediated signaling pathway
Biological Processinterleukin-4-mediated signaling pathway
Biological Processinterleukin-9-mediated signaling pathway
Biological Processintracellular signal transduction
Biological Processnegative regulation of dendritic cell cytokine production
Biological Processnegative regulation of FasL production
Biological Processnegative regulation of interleukin-10 production
Biological Processnegative regulation of interleukin-12 production
Biological Processnegative regulation of T cell activation
Biological Processnegative regulation of T-helper 1 cell differentiation
Biological Processnegative regulation of thymocyte apoptotic process
Biological Processpositive regulation of activated T cell proliferation
Biological Processpositive regulation of calcium ion transport
Biological Processpositive regulation of cytosolic calcium ion concentration
Biological Processpositive regulation of dendritic cell apoptotic process
Biological Processpositive regulation of extrinsic apoptotic signaling pathway in absence of ligand
Biological Processpositive regulation of nitric-oxide synthase biosynthetic process
Biological Processpositive regulation of T cell proliferation
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processprotein phosphorylation
Biological Processregulation of apoptotic process
Biological Processregulation of T cell apoptotic process
Biological Processresponse to interleukin-4
Biological ProcessT cell homeostasis
Biological Processtranscription by RNA polymerase II

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Tyrosine-protein kinase JAK3
  • EC number
  • Alternative names
    • Janus kinase 3 (JAK-3)

Gene names

    • Name
      Jak3

Organism names

  • Taxonomic identifier
  • Strain
    • Brown Norway
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    Q63272
  • Secondary accessions
    • F1LR79

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000881171-1100Tyrosine-protein kinase JAK3
Modified residue17Phosphoserine
Modified residue781Phosphotyrosine; by autocatalysis
Modified residue900Phosphotyrosine
Modified residue935Phosphotyrosine
Modified residue976Phosphotyrosine; by autocatalysis
Modified residue977Phosphotyrosine; by autocatalysis

Post-translational modification

Autophosphorylated, leading to regulate its activity. IL2 promotes phosphorylation on tyrosine residues, including autophosphorylation on Tyr-781 (By similarity).
Dephosphorylation of Tyr-976 and Tyr-977 by PTPN2 negatively regulates cytokine-mediated signaling (By similarity).

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

In contrast with the ubiquitous expression of the other JAKs, JAK3 is predominantly expressed in hematopoietic tissues.

Gene expression databases

Interaction

Subunit

Interacts with STAM2 and MYO18A (By similarity).
Interacts with SHB (By similarity).
Interacts with CD69 (By similarity).

Protein-protein interaction databases

Chemistry

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-223cytokine/interferon/growth hormone receptors
Domain24-353FERM
Domain372-472SH2; atypical
Domain517-777Protein kinase 1
Domain818-1091Protein kinase 2

Domain

Possesses two phosphotransferase domains. The second one contains the catalytic domain, while the presence of a pseudokinase domain is required for suppression of basal activity of JAK3.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,100
  • Mass (Da)
    122,726
  • Last updated
    2021-02-10 v2
  • Checksum
    F7FEBF8D91061C8A
MAPPSEETPLISQRSCSLSSSEAGALHVLLPPRGPGPPQRLSFSFGDYLAEDLCVRAAKACGILPVYHSLFALATEDLSCWFPPSHIFSIEDVDTQVLVYRLRFYFPGWFGLETCHRFGLHKDLTSAILDVHVLEHLFAQHRSDLVSGRLPVGLSLKDQGEFLSLAVLDLAQMARKQAQRPGELLKSVSYKACLPPSLRDLIQGQSFVTRRRIRRTVVQALRRVVACQADRYALMAKYILDLERLHPAATTESFLVGLPGAQEEPGCLRVTGDNGIAWSSKDQELFQTFCDFPEIVDVSIKQAPRVGPAGEHRLVTITRMDGHILEAEFPGLPEALSFVALVDGYFRLICDSRHFFCKEVAPPRLLEEEAELCHGPITLDFAIHKLKAAGSLPGSYILRRSPQDYDSFLLTACVQTPLGPDYKGCLIRQDPSGAFSLVGLSQLHRSLQELLTACWHSGLQVDGTALNLTSCCVPRPKEKSNLIVVRRGRNPTPAPGHSPSCCALTKLSFHTIPADSLEWHENLGHGSFTKIFHGHRREVVDGETHDTEVLLKVMDSRHQNCMESFLEAASLMSQVSYPHLVLLHGVCMAGDSIMVQEFVYLGAIDTYLRKRGHLVPASWKLQVTKQLAYALNYLEDKGLPHGNVSARKVLLAREGVDGNPPFIKLSDPGVSPTVLSLEMLTDRIPWVAPECLQEAGTLNLEADKWGFGATTWEVFSGAPMHITSLEPAKKLKFYEDRGQLPALKWTELEGLIAQCMAYDPGRRPSFRAILRDLNGLITSDYELLSDPTPGIPNPRDELCGGAQLYACQDPAIFEERHLKYISLLGKGNFGSVELCRYDPLGDNTGPLVAVKQLQHSGPEQQRDFQREIQILKALHCDFIVKYRGVSYGPGRQSLRLVMEYLPSGCLRDFLQRHRARLHNDRLLLFAWQICKGMEYLGARRCVHRDLAARNILVESEAHVKIADFGLAKLLPLGKDYYVVREPGQSPIFWYAPESLSDNIFSRQSDVWSFGVVLYELFTYSDKSCSPSTEFLRMMGPEREGSPLCHLLELLAEGRRLPPPSTCPTEVQELMQLCWSPNPQDRPAFDTLSPQLDALWRGSPG

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0G2K636A0A0G2K636_RATJak31099
A0A8L2UK66A0A8L2UK66_RATJak31097

Sequence caution

The sequence BAA05868.1 differs from that shown. Reason: Frameshift

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict893in Ref. 1; BAA05868
Sequence conflict981in Ref. 1; BAA05868
Sequence conflict1034in Ref. 1; BAA05868

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D28508
EMBL· GenBank· DDBJ
BAA05868.1
EMBL· GenBank· DDBJ
mRNA Frameshift
AABR07024869
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CH474031
EMBL· GenBank· DDBJ
EDL90763.1
EMBL· GenBank· DDBJ
Genomic DNA
CH474031
EMBL· GenBank· DDBJ
EDL90761.1
EMBL· GenBank· DDBJ
Genomic DNA
CH474031
EMBL· GenBank· DDBJ
EDL90762.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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