Q5T9L3 · WLS_HUMAN
- ProteinProtein wntless homolog
- GeneWLS
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids541 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins (PubMed:34587386).
Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).
Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameProtein wntless homolog
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionQ5T9L3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Multi-pass membrane protein
Cytoplasmic vesicle membrane ; Multi-pass membrane protein
Cell membrane ; Multi-pass membrane protein
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Golgi apparatus membrane ; Multi-pass membrane protein
Early endosome membrane ; Multi-pass membrane protein
Note: Co-localizes with the adaptin AP2A2 at distinct punctae.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-15 | Cytoplasmic | ||||
Sequence: MAGAIIENMSTKKLC | ||||||
Transmembrane | 16-36 | Helical; Name=1 | ||||
Sequence: IVGGILLVFQIIAFLVGGLIA | ||||||
Topological domain | 37-232 | Lumenal | ||||
Sequence: PGPTTAVSYMSVKCVDARKNHHKTKWFVPWGPNHCDKIRDIEEAIPREIEANDIVFSVHIPLPHMEMSPWFQFMLFILQLDIAFKLNNQIRENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTK | ||||||
Transmembrane | 233-253 | Helical; Name=2 | ||||
Sequence: VWFAMKTFLTPSIFIIMVWYW | ||||||
Topological domain | 254-268 | Cytoplasmic | ||||
Sequence: RRITMMSRPPVLLEK | ||||||
Transmembrane | 269-289 | Helical; Name=3 | ||||
Sequence: VIFALGISMTFINIPVEWFSI | ||||||
Topological domain | 290-303 | Lumenal | ||||
Sequence: GFDWTWMLLFGDIR | ||||||
Transmembrane | 304-324 | Helical; Name=4 | ||||
Sequence: QGIFYAMLLSFWIIFCGEHMM | ||||||
Topological domain | 325-331 | Cytoplasmic | ||||
Sequence: DQHERNH | ||||||
Transmembrane | 332-352 | Helical; Name=5 | ||||
Sequence: IAGYWKQVGPIAVGSFCLFIF | ||||||
Topological domain | 353-380 | Lumenal | ||||
Sequence: DMCERGVQLTNPFYSIWTTDIGTELAMA | ||||||
Transmembrane | 381-401 | Helical; Name=6 | ||||
Sequence: FIIVAGICLCLYFLFLCFMVF | ||||||
Topological domain | 402-431 | Cytoplasmic | ||||
Sequence: QVFRNISGKQSSLPAMSKVRRLHYEGLIFR | ||||||
Transmembrane | 432-452 | Helical; Name=7 | ||||
Sequence: FKFLMLITLACAAMTVIFFIV | ||||||
Topological domain | 453-471 | Lumenal | ||||
Sequence: SQVTEGHWKWGGVTVQVNS | ||||||
Transmembrane | 472-492 | Helical; Name=8 | ||||
Sequence: AFFTGIYGMWNLYVFALMFLY | ||||||
Topological domain | 493-541 | Cytoplasmic | ||||
Sequence: APSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE |
Keywords
- Cellular component
Disease & Variants
Involvement in disease
Zaki syndrome (ZKS)
- Note
- DescriptionAn autosomal recessive disorder characterized by developmental delay, progressive microcephaly, and short stature, as well as dysmorphic features including sparse scalp hair, cupped ears, wide nose and mouth, short philtrum, and high-arched palate. Other variable features have been observed, including ocular, skeletal, cardiac, and renal anomalies.
- See alsoMIM:619648
Natural variants in ZKS
Variant ID | Position(s) | Change | Description | |
---|---|---|---|---|
VAR_086565 | 392 | Y>C | in ZKS; results in decreased secretion of WNT3A and WNT5A; decreased activation of WNT signaling; dbSNP:rs2100452147 | |
VAR_086566 | 478 | Y>C | in ZKS; results in decreased secretion of WNT3A and WNT5A; decreased activation of WNT signaling; dbSNP:rs985347096 | |
VAR_086567 | 531 | I>T | in ZKS; results in decreased secretion of WNT3A and WNT5A; decreased activation of WNT signaling; dbSNP:rs2100377758 | |
VAR_086568 | 536 | R>C | in ZKS; results in decreased secretion of WNT3A and WNT5A; decreased activation of WNT signaling; dbSNP:rs773311381 |
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_086565 | 392 | in ZKS; results in decreased secretion of WNT3A and WNT5A; decreased activation of WNT signaling; dbSNP:rs2100452147 | |||
Sequence: Y → C | ||||||
Natural variant | VAR_029991 | 465 | in dbSNP:rs983034 | |||
Sequence: V → I | ||||||
Natural variant | VAR_086566 | 478 | in ZKS; results in decreased secretion of WNT3A and WNT5A; decreased activation of WNT signaling; dbSNP:rs985347096 | |||
Sequence: Y → C | ||||||
Natural variant | VAR_086567 | 531 | in ZKS; results in decreased secretion of WNT3A and WNT5A; decreased activation of WNT signaling; dbSNP:rs2100377758 | |||
Sequence: I → T | ||||||
Natural variant | VAR_086568 | 536 | in ZKS; results in decreased secretion of WNT3A and WNT5A; decreased activation of WNT signaling; dbSNP:rs773311381 | |||
Sequence: R → C |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 677 variants from UniProt as well as other sources including ClinVar and dbSNP.
Keywords
- Disease
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000271777 | 1-541 | Protein wntless homolog | |||
Sequence: MAGAIIENMSTKKLCIVGGILLVFQIIAFLVGGLIAPGPTTAVSYMSVKCVDARKNHHKTKWFVPWGPNHCDKIRDIEEAIPREIEANDIVFSVHIPLPHMEMSPWFQFMLFILQLDIAFKLNNQIRENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGISMTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAGYWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDIGTELAMAFIIVAGICLCLYFLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWKWGGVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE |
Post-translational modification
N-glycosylated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Interacts with WNT3A. Interacts with WNT1, WNT3 and WNT5A (By similarity).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q5T9L3 | OPRM1 P35372 | 11 | EBI-2868748, EBI-2624570 | |
BINARY | Q5T9L3 | SLC6A3 Q01959 | 2 | EBI-2868748, EBI-6661445 | |
BINARY | Q5T9L3 | WNT3A P56704 | 5 | EBI-2868748, EBI-6173037 | |
BINARY | Q5T9L3 | WNT5A P41221 | 3 | EBI-2868748, EBI-6594545 | |
BINARY | Q5T9L3 | WNT7A O00755 | 3 | EBI-2868748, EBI-727198 | |
BINARY | Q5T9L3-1 | ADORA2A P29274 | 3 | EBI-22114623, EBI-2902702 | |
BINARY | Q5T9L3-1 | AQP4 P55087 | 3 | EBI-22114623, EBI-10104898 | |
BINARY | Q5T9L3-1 | EMC7 Q9NPA0 | 3 | EBI-22114623, EBI-6309137 | |
BINARY | Q5T9L3-1 | GPM6A P51674 | 2 | EBI-22114623, EBI-7187133 | |
BINARY | Q5T9L3-1 | SGIP1 Q9BQI5 | 3 | EBI-22114623, EBI-2690801 | |
BINARY | Q5T9L3-1 | WLS Q5T9L3-1 | 3 | EBI-22114623, EBI-22114623 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 101-232 | Interaction with Wnt proteins | ||||
Sequence: MEMSPWFQFMLFILQLDIAFKLNNQIRENAEVSMDVSLAYRDDAFAEWTEMAHERVPRKLKCTFTSPKTPEHEGRYYECDVLPFMEIGSVAHKFYLLNIRLPVNEKKKINVGIGEIKDIRLVGIHQNGGFTK |
Sequence similarities
Belongs to the wntless family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q5T9L3-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length541
- Mass (Da)62,253
- Last updated2007-01-09 v2
- Checksum0DAFDF51BBA0C288
Q5T9L3-2
- Name2
Q5T9L3-3
- Name3
- Differences from canonical
- 36-127: APGPTTAVSYMSVKCVDARKNHHKTKWFVPWGPNHCDKIRDIEEAIPREIEANDIVFSVHIPLPHMEMSPWFQFMLFILQLDIAFKLNNQIR → G
Computationally mapped potential isoform sequences
There are 7 potential isoforms mapped to this entry
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_022346 | 36-37 | in isoform 2 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_046143 | 36-127 | in isoform 3 | |||
Sequence: APGPTTAVSYMSVKCVDARKNHHKTKWFVPWGPNHCDKIRDIEEAIPREIEANDIVFSVHIPLPHMEMSPWFQFMLFILQLDIAFKLNNQIR → G | ||||||
Sequence conflict | 136 | in Ref. 4; CAD98094 | ||||
Sequence: V → A | ||||||
Alternative sequence | VSP_022347 | 507-541 | in isoform 2 | |||
Sequence: DLGVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE → MQLPCKSREDCALFVSELYQELFSASKYSFINDNAASGI |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQ323735 EMBL· GenBank· DDBJ | ABD58927.1 EMBL· GenBank· DDBJ | mRNA | ||
AY359035 EMBL· GenBank· DDBJ | AAQ89394.1 EMBL· GenBank· DDBJ | mRNA | ||
AK074984 EMBL· GenBank· DDBJ | BAC11333.1 EMBL· GenBank· DDBJ | mRNA | ||
BX538320 EMBL· GenBank· DDBJ | CAD98094.1 EMBL· GenBank· DDBJ | mRNA | ||
BX648748 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
AL513284 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL157407 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH471059 EMBL· GenBank· DDBJ | EAX06480.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471059 EMBL· GenBank· DDBJ | EAX06481.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC110826 EMBL· GenBank· DDBJ | AAI10827.1 EMBL· GenBank· DDBJ | mRNA | ||
BC137109 EMBL· GenBank· DDBJ | AAI37110.1 EMBL· GenBank· DDBJ | mRNA | ||
BC137113 EMBL· GenBank· DDBJ | AAI37114.1 EMBL· GenBank· DDBJ | mRNA |