Q5GJ77 · GPAT1_BOVIN

  • Protein
    Glycerol-3-phosphate acyltransferase 1, mitochondrial
  • Gene
    GPAM
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

Mitochondrial membrane protein that catalyzes the essential first step of biosynthesis of glycerolipids such as triglycerides, phosphatidic acids and lysophosphatidic acids (By similarity).
Esterifies acyl-group from acyl-coenzyme A (acyl-CoA) to the sn-1 position of glycerol-3-phosphate, to produce lysophosphatidic acid (By similarity).
Has a narrow hydrophobic binding cleft that selects for a linear acyl chain (By similarity).
Catalytic activity is higher for substrates with a 16-carbon acyl chain (By similarity).

Catalytic activity

  • sn-glycerol 3-phosphate + an acyl-CoA = a 1-acyl-sn-glycero-3-phosphate + CoA
    This reaction proceeds in the forward direction.
    EC:2.3.1.15 (UniProtKB | ENZYME | Rhea)
  • (9Z,12Z)-octadecadienoyl-CoA + sn-glycerol 3-phosphate = 1-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphate + CoA
    This reaction proceeds in the forward direction.
  • sn-glycerol 3-phosphate + (9Z)-octadecenoyl-CoA = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA
    This reaction proceeds in the forward direction.
  • sn-glycerol 3-phosphate + octadecanoyl-CoA = 1-octadecanoyl-sn-glycero-3-phosphate + CoA
    This reaction proceeds in the forward direction.
  • sn-glycerol 3-phosphate + hexadecanoyl-CoA = 1-hexadecanoyl-sn-glycero-3-phosphate + CoA
    This reaction proceeds in the forward direction.
  • dodecanoyl-CoA + sn-glycerol 3-phosphate = 1-dodecanoyl-sn-glycerol 3-phosphate + CoA
    This reaction proceeds in the forward direction.
  • 1-acyl-sn-glycero-3-phospho-(1'-sn-glycerol) + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + CoA
    This reaction proceeds in the forward direction.

Pathway

Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site278CoA (UniProtKB | ChEBI)
Binding site279CoA (UniProtKB | ChEBI)
Binding site288CoA (UniProtKB | ChEBI)
Binding site293CoA (UniProtKB | ChEBI)
Binding site328CoA (UniProtKB | ChEBI)
Binding site461CoA (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmitochondrial membrane
Cellular Componentmitochondrial outer membrane
Cellular Componentplasma membrane
Molecular Functionglycerol-3-phosphate O-acyltransferase activity
Biological ProcessCDP-diacylglycerol biosynthetic process
Biological Processdiacylglycerol biosynthetic process
Biological Processfatty acid metabolic process
Biological Processglycerol-3-phosphate metabolic process
Biological Processphosphatidic acid biosynthetic process
Biological Processphosphatidylglycerol biosynthetic process
Biological Processphospholipid biosynthetic process
Biological Processtriglyceride biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glycerol-3-phosphate acyltransferase 1, mitochondrial
  • EC number
  • Short names
    GPAT-1

Gene names

    • Name
      GPAM
    • Synonyms
      GPAT1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos

Accessions

  • Primary accession
    Q5GJ77

Proteomes

Subcellular Location

Mitochondrion outer membrane
; Peripheral membrane protein
Note: Associated with the mitochondrion outer membrane of hepatic cells via a patch of basic residues.

Features

Showing features for topological domain, intramembrane.

TypeIDPosition(s)Description
Topological domain1-87Cytoplasmic
Intramembrane88-118
Topological domain119-825Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002450291-825Glycerol-3-phosphate acyltransferase 1, mitochondrial
Modified residue380Phosphoserine
Modified residue685Phosphoserine
Modified residue692Phosphoserine
Modified residue777N6-acetyllysine
Modified residue781N6-acetyllysine

Keywords

Proteomic databases

Expression

Tissue specificity

Highly expressed in adipose tissues and lung. Low expression in liver.

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, motif.

TypeIDPosition(s)Description
Region80-120Important for mitochondrial localization
Motif230-235HXXXXD motif
Region435-455Disordered

Domain

The HXXXXD motif is essential for acyltransferase activity and contributes to the binding of the cysteamine moiety of the acyl-CoA and the phosphate moiety of the glycerol-3-phosphate.

Sequence similarities

Belongs to the GPAT/DAPAT family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    825
  • Mass (Da)
    93,144
  • Last updated
    2005-03-01 v1
  • Checksum
    01D294B9DE03CC04
MDESALTLGTIDVSYLPNSSEYSIGRCKHATEEWGECGSRPTVFRSATLKWKESLMSRKRPFVGRCCYSCTPQSWDKFFNPSIPSLGLRNVIYINETHTRHRGWLARRLSYVLFIQERDVHKGMFATNVTENVLNSSRVQEAIAEVAGELNPDGSAQQQSKAVNKVKKKARKILQEMVATVSPAMIRLTGWVLLKLFNSFFWNIQIHKGQLEMVKAATETNLPLIFLPVHRSHIDYLLLTFILFCHNIKAPYIASGNNLNIPIFSTLIHKLGGFFIRRRLDETPDGRKDILYRALLHGHIVELLRQQQFLEIFLEGTRSRSGKISCARAGLLSVVVDTLSTNTIPDILIIPGGISYDRIIEGHYNGEQLGKPKKNESLWSIARGVIRMLRKNYGCVKTDFAQPFSLKEYLESQSQKPVSAPLSLEQALLPAILPSRPSGAADEGTDMSINESRNATDESRRRLIAHLAEHILFTASKSCAIMSTHIVACLLLYRHRQGIGLFTLVEDFFVMKEEVLARDFDLGFSGNSEDVVMHAIQFLGNCITITHTSKNDEFFITPSTTIPSVFELNFYSNGVLHVFIMEAIIACSLYAVLKKRGPGGPASPSLVSQEQLVHKAASLCYLLSNEGTISLPCQTFYQICHETVGRFIQYGILIVAEQDDQEDISPGLAEQQWDKKLPEPLSWRSDEEDEDSDFGEEQRDCYLKVSQSKEHQQFITFLQRLLGPLLEAYSSAAVFIHNFGGPVPEPEFLQKLHKYLITRTERRVAVYAESATYCLVKNAVKTFKDIGVFKETKQKRVSGLELSNTFLPQCNRQKLLEYILSLVVL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY515690
EMBL· GenBank· DDBJ
AAS79429.1
EMBL· GenBank· DDBJ
mRNA
AY945228
EMBL· GenBank· DDBJ
AAY24765.1
EMBL· GenBank· DDBJ
Genomic DNA
AY945226
EMBL· GenBank· DDBJ
AAY24765.1
EMBL· GenBank· DDBJ
Genomic DNA
AY945227
EMBL· GenBank· DDBJ
AAY24765.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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